MICROBIOLOGY SECTION The microbiology section aims at studying yu ailments and diseases from the isolation of suspected causative organisms. The processes involved in the isolation of these organisms include; culturing‚ staining‚ microscopy and sensitivity tests. Samples collected for examination include; stool‚ blood‚ sputum‚ urine‚ vaginal swab‚ wound swab and wound biopsy. Bacterial Culturing and sensitivity Cultures are carried out to isolate suspected organisms from a sample. There are different
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Salmonella enterica (#11) Gram Negative Organism | CarbohydrateFermentation(PRL) | CarbohydrateFermentation(PRG) | Indole productionTest | MethylRed Test(MR) | VogesProskauer Test | Citrate Utilization Test | Hydrogen sulfide Test | Urease Test | CatalaseTest | Salmonellaenterica | (-)Red color | (+)YellowColor | (-)No red ring | (+)RedRing | (-)No pink | (+)Blue With growth | (+)Black PrecipitateVery motile | (-)No change in color | (+) | The unknown organism (#11) was Salmonella
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colony characterization and were estimated by their morphological and biochemical characters. As being a monsoon the occurrence of variation of different species were high. The microorganisms isolated from the soil were of staphylococcus strain and were gram positive‚ aerobic‚ coccus shaped bacteria. The fungal species were also identified‚ of which Aspergillus and Penicillium were dominant‚ followed by mucur‚ as sub dominant .This project aims to find out the water and soil quality of River and as it
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the knowledge gained from the sessions in the laboratory‚ we can now integrate what we have learned to the process of finding out the unknowns given. Materials and Methods The professor gave out the unknown specimens. It contained one-‐gram positive and one-‐gram negative bacteria from the given list. I was assigned unknown A. The process of identification was achieved by utilizing procedures learnt during the semester. Procedures were followed as stated in the lab manual (1). Since the sample contained
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ferment the product can be stained with the gram staining. Gram staining is the method of differentiating bacteria into 2 large groups of Gram-positive bacteria and Gram-negative bacteria based on their cell wall. Most of the bacteria or yeast in the starter cultures used are Gram-positive. Gram staining requires 4 types of solutions used in the procedure: crystal violet‚ mordant‚ alcohol and safranin. The Gram-positive microbes will have blue stain while Gram-negative stains pink after the procedure
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* Microbiology: Study of microorganisms Most microorganisms are pathogenicFalse The majority of bacteria are harmless True Total number of bacterial species: about 10 million to a billion What is a microorganism? An organism or virus too small to be seen without a microscope. (Smaller than 0.5 mm) Ex: Bacteria‚ Fungi‚ Protozoa‚ Algae‚ and Viruses Microbiom: collection of microbes Human cells vs. Microbial cells = 1:10 Which statement about Michaelangelo is not correct? A. He was
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SUPERVISOR- PROFESSOR N. SMITH | Contents ANTIMICROBIAL PROPERTIES OF SOIL 2 ABSTRACT 2 LITERATURE REVIEW 3 AIM OF RESEARCH 4 OBJECTIVES OF STUDY 4 RESEARCH METHODOLOGY 4 METHOD(S) TO FACILITATE MICROBIAL IDENTIFICATION 5 Gram stain 5 Culturing 5 MATERIALS TO BE USED 5 ETHICAL CONSIDERATIONS 6 SAMPLE COLLECTION 6 SAMPLE ANALYSIS METHOD 7 DAY 3 – Expected Results 9 DISSEMINATION OF RESULTS 9 WORK AND TIME SCHEDULE 10 FINANCE BUDGET 10 CONCLUSION
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LABORATORY EXERCISE 8-A: Preparation of Bacterial Smear and Simple Staining I. INTRODUCTION Bacterial smears are prepared for the purpose of viewing microorganisms under the microscope. Visualization of microorganisms in the living state is very difficult‚ not just because they are minute‚ but because they are transparent and almost colorless when suspended in an aqueous medium. A bacterial smear is a dried preparation of bacterial cells on a glass slide. Smears may be made from a dry culture
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IMPORTANT BACTERIA | GRAM-POSITIVE BACTERIA * Alpha and gamma hemolysis on blood agar * Bacillus cereus colonies on blood agar * Beta-hemolysis on blood agar (S.aureus) * Beta-hemolysis on blood agar * Beta-hemolytic colonies on blood agar * Clostridium perfringens Gram stain * Corynebacterium diphtheriae Gram stain * Corynebacterium pseudotuberculosis on blood agar * Enterococcus faecalis * Enterococcus faecalis on blood agar * Enterococcus faecalis Gram stain * Enterococcus
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walls of bacteria‚ a Gram stain determines what kind of bacterium the cell is. The Gram stain works by creating a purple dye that either attaches or fails to attach to the peptidoglycan either found or not found on the cell wall of the cell. Gram-positive bacteria are cells that have a large amount of peptidoglycan in their cell wall‚ and those that are Gram-negative have an extra cell wall that does not allow the stain to set on the cell wall (Vidyasagar). In addition to staining the cell‚ the identification
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