Fermentation of Lactose Test‚ the Sulfide Indole Mobility (SIM) Test‚ the Nitrate Reduction Test‚ the Protein Hydrolysis Test‚ the Catalase Test‚ and the Cytochrome Oxidase Test. The microbes that were tested during this lab were: Escherichia coli‚ Bacillus cereus‚ the unknown‚ Proteus vulgaris‚ Staphylococcus epidermis‚ Enterobacter aerogenes‚ the control‚ and Pseudomonas fluorescens. The microbes tested during these various tests were looking for which would: reduce sulfur/produce sulfate‚ produce
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Microorganisms are classified according to their structure. By means of flow charts‚ diagrams and tables explain the differences between Viruses‚ Bacteria‚ Cyanobacteria‚ Achaea and Fungi. Bacteria or bacterium are unicellular microorganisms. They are essentially only a few micrometres long and form of various shapes including the spheres‚ rods and spirals. A BACTERIAL CELL Illustration courtesy of Wikipedia. A Virus (from the Latin noun virus‚ meaning toxic or poison) is a sub-microscopic
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mitosis and meiosis. 2) Differentiate gram-positive and gram-negative bacteria. a) A gram positive bacteria remains colored with gram staining even after washing with alcohol or acetone‚ whereas gram negative bacteria does not retain the stain when washed with alcohol or acetone. A gram positive bacteria outer membrane is absent and gram negative bacteria outer membrane is present. A gram positive bacteria’s peptidoglycan layer is 20-80 nm thick‚ whereas a gram negative bacteria’s cell peptidoglycan
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primarily esculin. The plate was then inverted and incubated at 37 oC for 24 hours. Bile salts‚ the selective agent‚ can allow only Enterococcus and group d streptococcus to hydrolyse esculin in the presence of bile salts‚ and inhibit growth of other gram positive organisms. Ferric citrate is an indicator. When the hydrolytic substrate esculin molecules are split‚ the product esculetin reacts with ferric citrate and forms a dark brown iron ions precipitate. Positive result indicates the organism is
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organism cannot grow‚ the maximum pH‚ above which the organism cannot grow‚ and the optimum pH‚ at which the organism grows best. Escherichia coli are one of several types of bacteria that normally inhabit the intestine of humans. It is normally a Gram-negative‚ rod-shaped bacterium. It is about to survive in physiologically high temperatures that allows up to excess of 50oC. (Daniel C‚ 2008). However it is not able to resist temperature like endospore. Therefore‚ the amount of bacteria would decrease
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turn the agar plate over and divide the plate into four quadrants and label the agar plate whether you used the E. coli or B. megaterium and number the quadrants 1 through 4. Please keep in mind that one pair will test the E. coli and Environment 1 or 2‚ and one pair will test B. megaterium and Environment 1 or 2. After‚ you will need to swab the E. coli and B. megaterium on two different nutrient agar plates using a sterile disposable inoculating loop. Remember not to dig in into the agar or the
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testing in order to determine the identity of the unknown organism. The tests performed to identify the unknown organism included Gram Staining‚ Fluid thioglycollate/aero-tolerance test‚ Methyl Red-Vogues Proskauer‚ SIM test‚ Lysine Decarboxylase test‚ Lactose Fermentation test‚ Simmons Citrate test‚ and TSI test. The Gram staining technique showed that the organism was Gram negative and bacilli shaped. The fluid thioglycollate test showed that the organism was a facultative anaerobe‚ meaning ATP can
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investigations of each unknown in Table 1 by completing the following steps: 1) Apply the stain to your first unknown slide and examine it under the microscope. 2) Record the shape of the bacteria‚ the arrangement of the bacteria‚ and the gram staining characteristics. 3) Analyze and record the G+C content of the sample by dragging the DNA tube that corresponds to the bacterial sample to the GC Content Measuring Apparatus. (Note: the identification of the DNA tubes may be confusing;
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Diagnosis of latent TB relies on the tuberculin skin test (TST) and/or blood tests. Antibiotic resistance is a growing problem in multiple drug-resistant tuberculosis (MDR-TB) infections. Prevention relies on screening programs and vaccination with the bacillus Calmette–Guérin vaccine. One third of the world’s population is thought to have been infected with M. tuberculosis‚ with new infections occurring in about 1% of the population each year.[4] More people in the developing world contract tuberculosis
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Dobrinka Atanasova Anthrax Anthrax is acute infectious disease that affects humans and other mammals. The causative agent of Anthrax is Bacillus anthracis. Bacillus anthrasic is a rod-shaped bacterium that forms spores (3). As we know‚ spores can survive many‚ many years in harsh conditions. Furthermore‚ the bacterium is also Gram-positive‚ encapsulated and aerobic (2). Anthrax can enter and affect the body via the skin‚ the lungs or gastrointestinal tract. The first one‚ the skin‚ is the least
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