"Gram staining bacillus megaterium" Essays and Research Papers

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    Trypticase Soy Agar plate to isolate individual colonies to be studied‚ tested and identified. After incubation of the individual TSA plates‚ the morphologies were viewed and noted and a Gram stain was completed on each individual bacterium‚ which will be referred to as Bacteria #1 and Bacteria #2. After the Gram reaction was determined on Bacteria #1 and Bacteria #2‚ different biochemical tests were done according to the dichotomous keys provided in the lab manual. All the tests were performed by

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    metabolic and biochemical process. The gram stain distinguishes between Gram positive and Gram negative bacteria based on the composition of the cell wall. The Gram stain procedure distinguishes between Gram positive and Gram negative groups by coloring these cells red or violet. Gram positive bacteria stain violet due to the presence of a thick layer of peptidoglycan in their cell walls‚ which retains the crystal violet these cells are stained with. Gram negative bacteria stain red‚ because it

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    LOVELY PROFESSIONAL UNIVERSITY CAPSTONE PROJECT REPORT TOPIC- ANTIMICROBIAL ACTIVITY OF DIFFERENT TYPES OF HONEY. PROJECT GUIDE- SUBMITTED BY- DR. AKSHAY GARG MOHIT KUMAR DEPT. OF BIOTECHNOLOGY REG. NO.- 10800037 ROLL NO- RB1R07B02 B.TECH BIOTECH.(8th sem.)

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    Microbiology! 1) Groups of Organisms (7): Eukaryote: TRUE nucleus 1. Fungi 4. Bacteria 2. Algae 5. Rickettsia 3. Protozoa ! Prokaryote: NO Nucleus Ribosome is the only Organelle 6. Mycoplasma (7. Viruses: No cell structure‚ ARE NOT CELLS) Microorganisms are: ! UBIQUITOUS! ! HARMLESS IN THEIR NORMAL HABITAT ! OPPORTUNISTIC (If they leave their normal habitat and get into us) 2) Roles of Microorganisms: (Bacteria and Fungi are “Semi-Good”) - digestion -

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    Escherichia coli—better known as E. coli—is a gram negative‚ rod shaped bacteria. It is relatively harmless‚ but can occasionally cause food poisoning. It can also provide Vitamin K2. It prevents the establishment of pathogenic bacteria‚ and is associated with or found in the intestinal organ. The antibiotic that E. coli is resistant to is Penicillin. Bacillus subtilis—better known as B. subtilis—is known as the hay bacillus or the grass bacillus. It is gram and catalase positive. It is rod-shaped‚ with

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    Microscopic observations were performed on four different microscope slides. Three of these observations were gram stains and the last observation was of an endospore staining. The unknown bacteria samples for the endospore stain were taken off of a nutrient agar plate and stained using the Bartholomew and Mittwer’s method. Gram stain samples were taken off of tryptic soy agar and stained using the gram stain procedure that is listed in the Willgoh and Bleakly manual (2). Physiological Tests All biochemical

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    two slides for gram staining and viewing under a microscope. {Either my gram-stain slides were bad or the microscopes I chose for viewing were not good. No bacteria were found under the microscope.} Prepare another gram stain of the unknown bacteria. {Gram-positive‚ coccus shaped bacteria were observed under the microscope. Also noted‚ the slide may have been inadvertently contaminated during the gram stain process by another person in the laboratory.} [I do think that the gram-stain result

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    * Length of L. lactis ranges from 0.5-1.5 micrometer (µm). * L. lactis does not produce spores (nonsporulating). * No capsule formation. * L. lactis grow in microaerophilic conditions * L. lactis are non motile. * L. lactis are Gram (+ive). * L. lactis are catalase (-ive). * L. lactis have low-GC content. * L. lactis are acid-tolerant * Optimum growth temperature is 30oC at pH 6.8. * L. lactis are not produce CO2 in MRS Broth. Colony Morphology: * No

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    B.TECH MBA(BIOTECH} ROLL NO. =63 INTRODUCTION Bacillus thuringiensis is a Gram-positive‚ soil-dwelling bacterium of the genus Bacillus. Additionally‚ B. thuringiensis also occurs naturally in the gut of caterpillars of various types of moths and butterflies‚ as well as on the dark surface of plants. B. thuringiensis was discovered 1901 in

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    difficult to work with and could not tolerate anyone telling him that his theories were wrong” (Robert 2). Anthrax was the first disease that Koch put his time in to study. Pollender‚ Rayer and Davaine discovered the Anthrax Bacillus. Koch wanted to prove that the bacillus was the cause of the disease. He used Mice to help conduct the experiment. His conclusion was the mice were killed when injected with the blood of a anthrax disease farm animal and healthy blood from farm animals survived. “By

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