(JOURNAL ARTICLE) By SITUMBEKO LIWELEYA (s213459531) Submitted in fulfilment of the requirements for the degree of BACHALOROUS TECHNOLOGIEA: BIOMEDICAL TECHNOLOGY At the At Nelson Mandela Metropolitan University Port Elizabeth‚ 2013. SUPERVISOR- PROFESSOR SMITH. N. Biotechnology Research International Journal Instruction Page Article Processing Charges Biotechnology Research International is an open access journal. Open access charges allow publishers to make the
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smear my bacteria on a liquid medium. I then proceeded to incubate the medium for 24-48 hours. 1. GRAM STAIN The next step I took in finding my unknown bacteria was to gram stain it. This is used to differentiate the bacteria. The different staining reagents are: crystal violet‚ grams iodine‚ acetone-alcohol‚ and grams safranin. Under the microscope it was a pink color‚ which means Gram Negative. Also‚ the shape was a rod. 2. KLIGER’S IRON
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an agar plate‚ drawed the loop very lightly over the surface while being careful not to break the surface. A zig-zag motion used. -The name wrote on the plate ‚then placed it’s into incubator in a 37 degree incubator for 48 hours. Result of Gram stain: Result of Isolation of bacteria in the following pictures : Disscusion : Microorganism are organism that are too small and cannot be seen with naked
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has more than 100 phytochemicals. It is used as sugar substitute. The extracts from leaves are used for the microbial assay and study the antimicrobial activity. The microbes used for the testing the activities are E.coli‚ Staphylococcus aureus‚ Bacillus subtilis‚ Salmonella typhi‚ Aspergillusniger‚ Candida parapsilosis‚ Trichophyton rubrum. With different concentrations of the extracts starting from 2.5µg/ml‚ 5.0µg/ml‚ 7.5µg/ml‚ 1.0µg/ml and the highest activity is shown by Staphylococcus aureus
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specimen. ♥ Correctly handling the specimen ♥ Quickly transporting the specimen to the lab. ♥ Once the specimen reaches the lab it is cultured and identified. Dr.T.V.Rao MD 3 Microbe Identification • Identification measures include: ♣ Microscopy (staining) ♣ growth on enrichment‚ selective‚ differential or characteristic media ♣ specimen biochemical test (rapid test methods) ♣ immunological techniques ♣ molecular (genotypic) methods. • After the microbe is identified for clinical samples it is
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intake of the people of Nsukka‚ Nigeria. Fish samples collected were identified and bacterial load of the samples determined using agar plate method. Differentiations and characterizations of various isolates were based on biochemical reactions and gram-staining technique. Frozen mackerel fish (Scomber scombrus) was used for the study. Invitro assay result revealed that the samples were predominantly contaminated by three bacteria species viz: Staphylococcus aureus‚ Escherichia coli and Lactobacillus plantarum
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any two (2) microorganisms that are not classified as prokaryotes are and The two (2) genera of bacteria that are capable of producing spores are and b) c) d) An example of a Gram positive bacteria is example of a Gram negative bacteria. and is an e) Three (3) common differential staining methods used in microbiology are ‚ and "Ubiquitous" in microbiology means The minimum inhibitory concentration (MIC) means Of more than three thousand (3000) species of bacteria‚ only 10%
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differential‚ and selective media. Differentiate bacteria based on their ability to hydrolyze starch. Materials: Plates of EMB‚ Starch and blood agar. Stool sample. Inoculating loop. Bunsen burner. Soil sample. Cotton soap. Skin sample. Gram iodine. Results: Starch agar: Special types of media: Type of medium Medium Appearance of medium and growth Selective and differential Eosin-methylene blue Klebsilla E.coli Enriched Blood agar Discussion:
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Abstract The primary focus of this lab was on microscopy and simple stains. Crystal violet and Carbol fuchsine‚ simple staining components‚ were used to stain the slide in order to see the different microbes in order to determine their cellular shape and identify unknown ones by comparing. Introduction Bacterial cells are usually colorless because cytoplasm‚ for the most part‚ is transparent. Since the bacteria are colorless‚ it is almost essential to add a stain to make the bacteria more visible
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Bordetella pertussis is the cause of pertussis which is also known as whooping cough. It is a gram negative pleomorphic bacillus. Pertussis is highly contagious and can be deadly to newborn infants because they do not have the immunity to protect against this disease. Every year there are outbreaks of pertussis especially in the high school age children‚ even though most have had the Tdap (tetanus‚ diphtheria and acellular pertussis) Vaccine. Humans are the only host to the Bordetella pertussis bacterium
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