Microbiology: ‘The Correct handling of Micro-organisms’ 1. Devise a title for each of the two experiments you did : (i)‚ Experiment 1 demonstrated the growth of bacteria when placed in liquid nutrient broth culture‚ the number of species present had increased in growth. .(1) (ii) Experiment 2 illustrated the growth of bacteria when placed on different surfaces of solid agar plates which included: nutrient agar‚ CLED agar and MacConkey agar; the number of species present also had increased in growth
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using DMSO (50% in water) as negative control by well diffusion method‚ against Bacillus subtilis‚ Staphylococcus aureus (gram positive)‚ Escherichia coli (gram negative) and Candida (yeast). Each well in the agar plate was seeded by (100 µL) from each tested compound or (50% DMSO). The plates were incubated at 37°C for 24 hours. the inhibition zone around each well in mm were measured‚ table 3. strain 3b 3d 3e Bacillus subtilis 2 4.3 3.3 Staphylococcus aureus - 3.67 3.67 Escherichia coli - - - Candida
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Lab Report: Antibiotic Evaluation by the Kirby-Bauer Method Introduction Chemical antimicrobial agents are chemical compounds capable of either inhibiting the growth of microorganisms or killing them outright. Those which are taken internally to alleviate the symptoms of or promote healing from disease are called chemotherapeutic drugs‚ and among these is a class of compounds called antibiotics. In order for a chemotherapeutic drug to be classed as an antibiotic‚ it must be produced by
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Bacillus thuringiensis Bacillus thuringiensis is a universal‚ gram-positive‚ vegetative‚ spore-forming bacterium that is rod shaped and varies between 2–5 µm long and about 1.0 µm wide . Bt consists large rods with slightly curved ends and forms a parasporal crystal during the stationary phase of its growth cycle . Occurring in pairs or short chains‚ Bt is also motile with peritrichous flagella and non-caspulated . Bt belongs to the family of bacteria‚ Bacillus cerus (B. cerus)‚ which produces
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William Howard Taft Born: 9/15/1857 Birthplace: Cincinnati‚ Ohio William Howard Taft was born in Cincinnati on Sept. 15‚ 1857. A Yale graduate‚ he entered Ohio Republican politics in the 1880s. In 1886 he married Helen Herron. From 1887 to 1890‚ he served on the Ohio Superior Court; 1890–92‚ as solicitor general of the United States; 1892–1900‚ on the federal circuit court. In 1900 McKinley appointed him president of the Philippine Commission and in 1901 governor general. Taft had great success
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heated- treat to kill disease causing pathogen through a process of pasteurization. Pasteurization kills most pathogen‚ increases the product’s shelf life and preserve a natural taste of milk. After pasteurization‚ nonpathogenic bacteria such as Bacillus which is gram positive‚ rod shaped bacteria still present in milks and will metabolize protein into ammonia product and raise the milk’s pH. Other bacteria such as lactobacillus and Streptococcus ferment lactose to lactic acid and acetic acid. Those acids
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produce a magnified image? How does a lens produce a magnified image? 3) What are some ways to increase contrast in microscopy? 4) Define: simple stain‚ differential stain and special stain. 5) What are the steps in a gram stain? 6) How can staining aid in the diagnosis of TB? 7) In general‚ can viruses be imaged with a light microscope? 8) Are all bacteria microscopic (beyond the ability to see with the naked eye)? Prokaryotic structure and function 1) What
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prions protein‚ no genetic material 2. Cell morphology: determine by gene and environment conditions ⁃ coccus spherical ⁃ diplococci two ⁃ streptococci chain ⁃ sarcina cuboidal ⁃ tetrad four ⁃ staphylococci grape clustered ⁃ bacillus rod-like ⁃ coccobacillus oval ⁃ diplobacilli two ⁃ streptobacilli chain ⁃ palisades ladder ⁃ spirillum spiral < 20 turns ⁃ spirochete spiral > 20 turns ⁃ pleomorphic variation in size and shape 3. Cell structure ⁃ appendages
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Bacillus anthracis This article came from the New England Journal of Medicine and was written after‚ the Anthrax scare so that Physicians would have a better understanding of the origin‚ signs/symptoms and treatment of the bacterial infection B. anthracis. This is a gram positive bacteria which is found in soil and/or water where it can lie dormant until contact with host. Portal of entry can be from a cut‚ inhaled‚ ingested or a biological vector. An open wound is always susceptible to infection
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Clostridium difficile. The pathogenesis of enteric clostridial infection requires two factors; the presence of these microorganisms in the intestine and a change in the enteric microenvironment. C. perfringens is a Gram-positive‚ spore-forming‚ obligately anaerobic‚ non-motile bacillus and capable of toxin production. Materials and methods Case history: A male Maltese dog‚ weighing 2.04 kg‚ was died on the way to the animal hospital with clinical signs of weakness‚ large volume of bloody diarrhea
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