1. Describe the major differences between gram positive and gram negative bacteria cell walls. The gram negative bacteria cell wall is a thin peptidoglycan layer and an outer cell membrane with a lipopolysaccharide layer. The gram positive bacteria cell wall is a single thick peptidoglycan layer. This wall forms in a mesh like formation of three layers of alternating material. 2. From the procedure that you have carried out‚do you feel that the Gram positive stain is a simple procedure? No‚
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TITLE Lab #4 Soil –vs- Water Microbiology INTRODUCTION There are 4 learning goals for this lab: 1. Collect samples and compile data from at least 2 sources (soil; water) of your choice. 2. Identify at least 2 Prokaryotes (bacteria) and 2 Eukaryotes (fungus) in your samples. 3. Research the importance of bacteria and fungus to Earth in a non-medical context. 4. Compile a high-quality‚ 3+ resource (excluding the lab worksheet)‚ MLA-cited in-text laboratory report using the scientific method
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Name: Danielle Title: Unknown Lab Report Introduction: There are many reasons for identifying an unknown bacterium. The reasons range from medical purposes‚ such as determining if the unknown could cause ailments in living things or knowing what microorganisms are needed to make antibiotics. The experiment was done by applying methods in order to identify an unknown bacterium. An unknown bacterium was handed out by Dr. Honer. The appropriate tests were prepared and applied. The
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the outmost confidence‚ Proteus vulgaris had the precise qualifications. The point of this report is to further explore the identification of my unknown bacteria by revealing the results of the experiments and comparing them to the other six known bacteria: Micrococcus luteus‚ Staphylococcus aureus‚ Staphylococcus epidermidis‚ Alcaligenes faecalis‚ Escherichia coli‚ and Proteus vulgaris that were used in the lab‚ as well as comparing and contrasting the actual and factual results. Its unique pink rod
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Microbiology MCB 2010 Fall 2013 First Day of Classes: August 19 Last day to receive full refund: August 23 Last day to withdraw with grade of "W": October 24 Week 1 Topic Online Orientation Introduction to Microbiology (The Microbial World and You) The Unity of Living Systems (Anatomy of Eukaryotic and Prokaryotic Cells) Reading Assignments Chapter 1 Assignments to Submit Orientation Quiz Quiz 1 Quiz 2 Due Date Aug. 26 2 Chapter 4 Sept. 2 3 4 5 6 7 Chapters 5 and 6 Metabolism (Energy
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PRE LAB REPORT Reynaldo Riboul TLC and Column Chromatography October 6‚ 2013 Table of Chemicals: Chemical Hazards Mol. Wt. Density Grams Moles Acetone Flammable‚ Irritant 58.08 g mol−1 0.791 g cm−3 2.0 g 0.0344 Hexane Flammable‚ Irritant‚ Dangerous to Environment 86.18 g mol−1 .6548 g mL−1 9.0 g 0.1044 Fluorene Very toxic to aquatic life with long lasting effects 166.223 g/mol 1.202 g/mL 0.3 g 0.00180 Fluorenone Irritant 180.20 g mol−1 1.13 g/cm3 0.3 g 0.00166
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Lab Practical 2 Morphological Staining Techniques Simple Stain- Uses 1 Stain 1. Acid Stains (- Charge)- Stains Background- Nigrosin‚ India Ink and Congo Red 2. Base Stains (+ Charge)- Stains Cell- Methylene Blue‚ Crystal Violet‚ Safranin Differential Staining Techniques- Any Staining Technique using 2 or more stains is differential. It allows us to differentiate between parts. 1. Gram Stain- Two Stains‚ PLUS Reagents- Distinguishes Chemical Composition of Cell Wall PG only (+ Purple)
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Review Questions 1. Which type of S. pneumoniae strain is the most virulent‚ encapsulated or unencapsulated? 2. What allows S. pneumoniae to adhere to cell walls‚ phosphorylcholine or polysaccharide? 3. Which infections are most commonly caused by S. pneumoniae‚ arthritis & peritonitis or meningitis & bacteremia? both 4. What can society do to help protect itself from antimicrobial resistance‚ increase or decrease antimicrobial usage? Decrease usage 1. Staphylococcus aureus‚
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(2005). Evidence for functional overlap among multiple bacterial cell division proteins: compensating for the loss of FtsK. Mol Microbiol 58: Pages 596–612 Gordon‚ G.S Grainge I. (2010). FtsK – a bacterial cell division checkpoint? Molecular Microbiology Volume 78‚ Issue 5‚ pages 1055–1057 Grainge I.‚ Lesterlin C Griffiths A. J. F.‚ Wessler S. R.‚ Lewontin R. C. and Carroll S. B. (2008). Introduction to Genetic Analysis. W.H. Freeman and COmpany. Chapter 7: DNA: Structure and Replication. pages
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Gram Stain: The important part of this experiment is being able to determine a bacterium based on its cell wall structure. It also helps indentify if the unknown organism is a Gram positive or Gram negative. This is the initial step that must be taken before any other lab procedure may continue on to ensure the purity is present‚ the arrangement of the cells‚ and the shape the cell has. The staining of the cell starts off with using the primary stain‚ Crystal Violet which is a purple color‚ to begin
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