Gram Stain: Gram is an empirical method to distinguish the species of bacteria into two groups Gram-positive and Gram-negative based on the physicochemical properties of the cells. First‚ a smear was prepared by use a sterile transfer loop that been flamed to removes some bacteria from slant agar and placed on the slide; mixed with one drop of water and let air dried. After dried‚ heat fixation the slide by passed the slide over a flame quickly 2-3 times to stick bacteria to the slide. Next‚ the
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TENS 2146 Electric Devices and Measurements Lab 3 Current and Voltage This report was prepared by: L. Wall Fall 2009 Prof. R. Alba-Flores Team Members: J. White‚ L. Wall Conducted on: September 17‚ 2009 Submitted on : September 24‚ 2009 Abstract: In this lab students experimented with light emitting diodes. The student built a basic circuit with two LED’s and resistors in parallel. The results showed that the voltage is the same in parallel. The items that were in series had the
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Introduction The purpose of this lab is to determine which pigments in a plant support or effect photosynthesis‚ based on starch production‚ which wavelengths of light are involved in photosynthesis‚ and identify plant pigments found in a plant leaf by means of paper chromatography. Life on Earth is dependent entirely on the energy from the Sun‚ not only to keep the planet at a suitable temperature but also to provide the energy required to sustain life. The energy of the Sun‚ in the form of
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Method • Methodology is performed through 3 successive days: First Day: Trypan Blue cell viability assay: Description Trypan blue staining is a simple way to evaluate cell membrane integrity (and thus assume cell proliferation or death) but the method is not sensitive and cannot be adapted for high through put screening. Short 96 well assay: EACH condition should be done in triplicate or more. Steps 1. Trypsinize one T-25 flask and add 5 ml of complete media to trypsinized cells. Centrifuge
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Shakira Jarvis Microbiology Lab Assignment Laboratory Assignment Outline 1. Check in & The Microscope a. Review of proper lab etiquette. i. Review laboratory syllabus and b. Review of the Parts of a Microscope ii. Review of lab exercises about different types of Microscopes 2. Survey of Microorganisms c. Viewing‚ drawing‚ and describing several types of fungi‚ algae‚ and Bacteria iii. Chlamydomonas iv. Spirogyra
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MICROBIOLOGY 3444-007 Gram Negative Unknown Unknown Bacteria #14 LeNaiya Kydd 4/2/2014 Abstract In order to be able to identify the unknown organism that was given to us‚ we had to conduct a number of different tests. These biological tests are used because they help us be able to identify the properties of the unknown we have and be able to compare our observed results with actual results of all the potential organisms. When all of the data of the test are put together it is easy to
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any equations that may be encountered and go over their derision. Methodology: Reference the lab manual here. If you made any changes to the experiment make note of them here. Also list any precautions one should take with the chemicals being used. Give a brief overview of the procedural steps (others must be able to follow) Data: Place any measured values or observations made during the lab in this section. Include any known values that were looked up (don’t forget a reference for them)
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Stainin g What is Cellular Staining? Cell staining is a technique that can be used to better visualize cells and cell components under a microscope. By using different stains‚ one can preferentially stain certain cell components‚ such as a nucleus or a cell wall‚ or the entire cell. Most stains can be used on fixed‚ or non-living cells‚ while only some can be used on living cells; some stains can be used on either living is or non-living What Cellularcells. Staining? The most basic reason that cells
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Lab #5-Enzymes NAME DATE LAB PERIOD Introduction Enzymes are proteins‚ though highly complex and diverse‚ they serve one basic function; to work as an organic catalyst. A catalyst‚ as defined by Merriam-Webster dictionary‚ is a substance that enables a chemical reaction to proceed at a usually faster rate ("Catalyst-Definition and more."). They function by reducing the activation energy‚ or energy required to start a reaction. The way enzymatic reaction works cannot be altered‚ but the
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Lab Report #1: Observing Bacteria Microbiology Abstract: This lab exercise familiarized the student with the use of a microscope by observing and identifying various different slides under the microscope. The student practiced observing the given slides under the 10x‚ 40x‚ and 100x (oil immersion) objective lenses‚ which allowed for the identification of the different organism’s shapes and sizes. Purpose: The aim of this exercise is to equip the student with the knowledge
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