the knowledge gained from the sessions in the laboratory‚ we can now integrate what we have learned to the process of finding out the unknowns given. Materials and Methods The professor gave out the unknown specimens. It contained one-‐gram positive and one-‐gram negative bacteria from the given list. I was assigned unknown A. The process of identification was achieved by utilizing procedures learnt during the semester. Procedures were followed as stated in the lab manual (1). Since the sample contained
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incubated at 37C for 24- 48 hours‚ and two separate morphologies were then observed and recorded‚ unknown A and unknown B‚ a Gram stain was then performed for each unknown. After determining the Gram reaction‚ alternate staining procedures were performed as needed. Unknown A required further endospore staining‚ whereas unknown B required no further staining procedures. Once all staining methods were complete‚ specific biochemical tests were performed. The biochemical tests were chosen from the unknown
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ferment the product can be stained with the gram staining. Gram staining is the method of differentiating bacteria into 2 large groups of Gram-positive bacteria and Gram-negative bacteria based on their cell wall. Most of the bacteria or yeast in the starter cultures used are Gram-positive. Gram staining requires 4 types of solutions used in the procedure: crystal violet‚ mordant‚ alcohol and safranin. The Gram-positive microbes will have blue stain while Gram-negative stains pink after the procedure
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Bacterial Smears Are Fixed before Staining to? Answer It is important to heat fix the bacterial smear before staining so as to‚ kill the bacteria‚ firmly adhere the smear on to the microscopic slide to prevent washing off during staining‚ and to allow the sample to readily take up the stain. Reference: www2.hendrix.edu What is the purpose of heat- fixing the smear? It helps the cells adhere to the slide so that they can be stained. The purpose of heat fixing is to kill the organisms without
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performed within a certain time frame the specimen will become contaminated. Part II: Bacterial Anatomy The purpose of this experiment is to become familiar and gain an understanding of how morphology and the arrangement of bacteria relate to staining techniques. Sample 1 In this study a female patient who had a problem that had arisen suddenly after lunch‚ had allergic reaction to something that she ate or drank had sent her to the bathroom with cramps and copious diarrhea. She knew she
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_Control_______ variable. Through the semester‚ you have used two bacteria that produce colorful colonies: one produce yellow colonies‚ another one pink. What are the names of these two bacteria? Are they Gram+ or Gram-? Micrococcus Luteus‚ yellow colonies‚ gram + Serratia Marcescens‚ pink colonies‚ gram - There was one bacterial
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followed‚ they provide accurate data. 2. In 1884‚ Hans Christian Gram described a method of staining bacterial cells while not staining surrounding animal tissues; however‚ he thought the staining method he developed was faulty because not all bacteria stained. In a letter to the editor of the journal in which Gram published his findings‚ write your response to Gram’s concern. 3. Compare and contrast gram-positive and gram-negative cell walls with regard to (a) sensitivity to antimicrobial
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and evident in rat bite fever Exercise 3: Gram stain What is the function of the iodine solution in the Gram stain? If it were omitted‚ how would staining results be affected? Omitting the iodine in the gram’s stain would mean‚ the crystal violet that goes into the cell would be incapable of forming the Crystal Violet Iodine (CVI) composite. The CVI complex molecule possesses a bigger size than that of Crystal violet molecule; therefore‚ gram-positive bacteria are able to maintain the stain
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Bacteria Morphology April 2‚ 2015 Abstract – The Purpose of this exercise is to gain experience in bacterial morphologies in prepared wet-mounted slides and interpreting the findings of bacteria through direct and indirect staining technique. Hypothesis – The experiment will allow for further insight into stained organisms‚ allowing extended contrast‚ differentiating shape and structure through utilization of microscope. Procedure – Instructions followed as per procedure‚ specimens
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Robert Suran May 1 2013 Microbiology Abstract Over a three week time span I was able to successfully identify an unknown microorganism by combining the results of several tests. The first test was the Gram stain test which showed the microorganism was Gram positive and rod shaped. Using an Unknown Identification Flowchart‚ I proceeded to inoculate a Starch plate. In week 2‚ I analyzed the Starch plate and was able to determine that the results for Starch Hydrolysis were negative
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