antibiotic that works on gram negative bacteria. Gram-negative bacteria are bacteria that do not retain crystal violet dye in the Gram staining protocol whereas Gram-positive bacteria are those that are stained dark blue or violet by Gram staining. The bacteria we used were E-coli that are a gram negative bacteria and the only antibiotic out of Penicillin and Streptomycin that will work is Streptomycin. Penicillin is also effective antibiotic but it will only work on bacteria that Is gram positive because
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In March 10‚ the first procedure that was done was gram staining. The purpose of this procedure was to determine if the unknown bacterium is gram-positive or gram-negative‚ and also to determine the cell shape. Gram-positive cells will be purple while gram-negative cells will be pink. The methods for the Gram staining method first starts with a fixed smear of the bacterium‚ covered it with crystal violet for 30 seconds. Afterwards‚ it should be gently rinsed off with distilled water and be covered
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Using the Powersoil DNA extraction kits‚ one gram of soil will be used with six individual reagents to purify the sample of any impurities that might interfere with our results. The six reagents used are labeled as C1‚ C2‚ C3‚ C4‚ C5‚ and C6‚ each with their individual properties to unpurified unwanted
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bacteria? Gram Stain This separation is based on differences in what physical trait? The structure of the cell walls. What is the iodine used for in this staining technique? Crystal violet is first used to stain the sample and iodine is used to affix it. Why is it important to heat fix the bacteria prior to the staining procedure? This procedure is done to kill any bacteria that may be on the slide already and this will insure a clean slide. If you had a bacteria sample that you knew to be gram positive
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bacteria E.coli was the only type found in the given area by gram staining. E.coli was the chosen bacteria for this type of experiment. It is a gram negative bacterium that will grow rapidly given ‘any culture medium with the necessary energy source‚ nutrients‚ pH‚ and temperature’. Therefore‚ MacConkey Agar being the medium for its growth will enable us to achieve the experiment outcome. MacConkey Agar only allows the growth of gram negative bacteria as they do not contain bile salts and crystal
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Microbiology Laboratory Report Identification of Unknown Bacteria 6O BIO 251-7H1 Gram Stain Summary: Out of 28 Unknown microorganisms‚ slant 6O was randomly selected‚ and subjected to testing in order to identify it’s species. Seventeen of the unknown bacteria were classified as Gram-negative bacilli: Corynebacterium pseudodiphtheriticum‚ Enterobacter aerogenes‚ Eschirichia coli‚ Flavobacter capsulatum‚ Helicobacter pylori‚ Klebsiella pneumonia‚ Klebsiella oxytoca‚ Proteus hauseri‚ Proteus
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When gram stained gram-negative bacteria stains pink or red; it is colorless and requires safranin as a counterstain. The cell wall of the bacterial cell is a complex‚ semirigid structure responsible for the shape of the cell (Tortora‚ Funke & Case‚ 2013). Gram positive bacteria occupy a multilayered thick peptidoglycan‚ and teichoic acid. Teichoic acids assist with distinguishes gram positive bacteria from other bacteria. The Gram positive bacteria are easier to kill. The gram reaction of
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Review Sheet Exercise I: Survey of Higher Microorganisms: Protozoa‚ Fungi‚ and Helminths Protozoa (group of Kingdom Protista) 1. Amoeba a. nucleus- dark center of the cell b. food vacuole- They feed by taking nutrients into the cell by diffusion and packaging it into (clear circles spread throughout the cell) c. pseudopod- “false foot”; the motility results from the streaming of the protoplasm that forms the process 2. Entamoeba causes amoebiasis or amoebic dysentery‚
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identify a Gram(+) as well as a Gram(-) from the species that were given and the experiment proceeded as follows. METHODS The two streak plates made from the mixture of unknown bacteria showed two different colony morphologies. Colonies of Bacteria A were translucent in color‚ circular in form‚ with entire margins and a smooth surface. Colonies of Bacteria B were cream colored‚ irregular in form‚ with undulate margins and an umbonate surface. Two different morphologies allowed Gram stains to be
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most important tests done was gram staining because it helped to limit the possible bacterium that it could have been. To be sure which bacterium was worked with we referred to the Bergey’s manual and compared the results to the possible bacterium. Results:
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