investigations of each unknown in Table 1 by completing the following steps: 1) Apply the stain to your first unknown slide and examine it under the microscope. 2) Record the shape of the bacteria‚ the arrangement of the bacteria‚ and the gram staining characteristics. 3) Analyze and record the G+C content of the sample by dragging the DNA tube that corresponds to the bacterial sample to the GC Content Measuring Apparatus. (Note: the identification of the DNA tubes may be confusing;
Premium Bacteria DNA RNA
Lab Practical 2 Morphological Staining Techniques Simple Stain- Uses 1 Stain 1. Acid Stains (- Charge)- Stains Background- Nigrosin‚ India Ink and Congo Red 2. Base Stains (+ Charge)- Stains Cell- Methylene Blue‚ Crystal Violet‚ Safranin Differential Staining Techniques- Any Staining Technique using 2 or more stains is differential. It allows us to differentiate between parts. 1. Gram Stain- Two Stains‚ PLUS Reagents- Distinguishes Chemical Composition of Cell Wall PG only (+ Purple)
Premium Enzyme Gram staining Bacteria
Microbiology Department of Microbiology University of Kwa-zulu Natal 25 October 2010 ABSTRACT Different types of bacteria in various forms are found all around us‚ and it is a microbiologist’s job to be able to identify these bacteria. Using various staining techniques and physiological tests‚ an isolated bacterium can be identified. In this experiment‚ a single bacterial colony was isolated form Mycorrhizal spores‚ and further tests done on that colony. Sub culturing was done after each week to ensure
Premium Enzyme Gram staining Bacteria
2. Material and methods 2.1 Sample collection The following 14 samples of Staphylococcus aureus were used in this study. All samples were obtained from food contact surfaces at three food premises in University of Putra Malaysia (UPM). Samples were collected from the surface of each material according to standard swabbing techniques. The collection was carried out during weekdays (15:00-17:00) after finished the preparation of foods. Sterile swabs were removed from the coded test tubes that contain
Premium DNA Gene Protein
Bibliography: © The McGraw−Hill Companies‚ 2002 E X E RC I S E © The McGraw−Hill Companies‚ 2002 Gram Positive 38. General Unknown © The McGraw−Hill Companies‚ 2002
Premium Microscope Microbiology Microorganism
Trypticase Soy Agar plate to isolate individual colonies to be studied‚ tested and identified. After incubation of the individual TSA plates‚ the morphologies were viewed and noted and a Gram stain was completed on each individual bacterium‚ which will be referred to as Bacteria #1 and Bacteria #2. After the Gram reaction was determined on Bacteria #1 and Bacteria #2‚ different biochemical tests were done according to the dichotomous keys provided in the lab manual. All the tests were performed by
Premium Bacteria Escherichia coli Microbiology
metabolic and biochemical process. The gram stain distinguishes between Gram positive and Gram negative bacteria based on the composition of the cell wall. The Gram stain procedure distinguishes between Gram positive and Gram negative groups by coloring these cells red or violet. Gram positive bacteria stain violet due to the presence of a thick layer of peptidoglycan in their cell walls‚ which retains the crystal violet these cells are stained with. Gram negative bacteria stain red‚ because it
Premium Bacteria Adenosine triphosphate
Microbiology! 1) Groups of Organisms (7): Eukaryote: TRUE nucleus 1. Fungi 4. Bacteria 2. Algae 5. Rickettsia 3. Protozoa ! Prokaryote: NO Nucleus Ribosome is the only Organelle 6. Mycoplasma (7. Viruses: No cell structure‚ ARE NOT CELLS) Microorganisms are: ! UBIQUITOUS! ! HARMLESS IN THEIR NORMAL HABITAT ! OPPORTUNISTIC (If they leave their normal habitat and get into us) 2) Roles of Microorganisms: (Bacteria and Fungi are “Semi-Good”) - digestion -
Premium Bacteria
Microscopic observations were performed on four different microscope slides. Three of these observations were gram stains and the last observation was of an endospore staining. The unknown bacteria samples for the endospore stain were taken off of a nutrient agar plate and stained using the Bartholomew and Mittwer’s method. Gram stain samples were taken off of tryptic soy agar and stained using the gram stain procedure that is listed in the Willgoh and Bleakly manual (2). Physiological Tests All biochemical
Premium Gram staining Microbiology Bacteria
two slides for gram staining and viewing under a microscope. {Either my gram-stain slides were bad or the microscopes I chose for viewing were not good. No bacteria were found under the microscope.} Prepare another gram stain of the unknown bacteria. {Gram-positive‚ coccus shaped bacteria were observed under the microscope. Also noted‚ the slide may have been inadvertently contaminated during the gram stain process by another person in the laboratory.} [I do think that the gram-stain result
Premium Bacteria Escherichia coli Gram staining