Microbiology: study of small living things study of bacteria: bacteriology study of viruses: virology study of parasites: parasitology study of fungi: mycology NOT ALL BACTERIA ARE PATHOGENS (disease causing) antibiotic: used to treat bacterial infections and diseases (penicillin‚ mold‚ 1929) bioremediation: using bacteria to clean up toxins pathogenesis: ability of an organism to be pathogenic virulence factors: toxins‚ receptors‚ cellular composition innate immune response v.s
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harvard.edu/node/222 BSP‚ S. (2010). How is EM different from light microscopy? Retrieved April 25‚ 2015‚ from http://bsp.med.harvard.edu/node/222 Tortora‚ G.‚ Funke‚ B.‚ & Case‚ C. (2010). Staining Techniques. Retrieved April 25‚ 2015‚ from http://www.cliffsnotes.com/sciences/biology/microbiology/microscopy/staining-techniques
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appear edged in black and are very bright and refractile. Endospores strongly resist application of simple stains or dyes and hence appear as nonstaining entities in Gram-stain preparations. However‚ once stained‚ endospores are quite resistant to decolorization. This is the basis of several spore stains such as the Schaeffer-Fulton staining method which also differentiates the spores from sporangia and vegetative cells. However this method is not suitable for taxanomic purposes.
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Activities/Tasks/Responsibilities | Problem(s) Encountered | Action(s) Taken | Personal Reflection | BacteriologyJuly 4- July 31 | Receiving of specimen‚ Logging‚ Processing of specimen‚ Inoculation into media‚ biochemical testing‚ identification of organism‚ gram staining‚ AFB staining‚ releasing of results‚ culture sensitivity testing. | No major problems were encountered during my rotation for Bacteriology. Minor problems like unfamiliarization of SOP’s and other procedures are encountered. | Asking proper procedures
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inoculating loop or needle‚ Bunsen burner‚ glass slides‚ lens paper‚ bibulous paper‚ regular tap water‚ along with reagents Crystal violet‚ Gram’s Iodine‚ 95% ethyl alcohol‚ and safranin. The first test I conducted was the gram stain. In preparation for the actual gram staining process I had to make a smear. To do this‚ I acquired a clean glass slide. I then placed one drop of water onto the center of the slide. Using the flame from my Bunsen burner I sterilized my loop by passing the malleable
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results‚ the isolated bacteria were tentatively identified as Agrobacterium strains. B.Characterization of Agrobacterium tumefaciens: Biochemical tests: biochemical features of the selected isolates were given in the table 1. The Gram reaction indicates selected isolated are Gram negative. All the six isolated are positive
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ATM (STP). A Gram stain was then carried out to differentiate the unknown sample from a broad class to a more specific category of bacteria. The Gram stain distinguishes between Gram-positive and Gram-negative bacteria based on the composition of the cell walls. Gram-positive bacteria appear purple and Gramnegative bacteria appear pink after staining. The first Gram stain produced unsatisfactory results and was then repeated with a clear indication of negativity. Light pink staining was evident
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3.3 Microbiological Analysis 3.3.1 Bacteria count using Heamocytometer This method was used to determine the of three types of dessert but does not differentiate the type of bacteria. 25 g of food samples will be homoginesed in a sterile stomacher bag and shaken for two minutes with 225 ml of peptone water to obtain the food mixture. Using separate sterile pipets‚ decimal dilutions of 10-2‚ 10-3‚ 10-4‚ 10-5 will be prepared and others as appropriate‚ of food homogenate by transferring 10 ml of previous
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and one result was inconclusive. The focus of this paper will be on the description of the just one of the three bacteria and some of its effects on humans. The first test done was a differential stain (gram stain) to determine the unknown microorganism cell shape and arrangement. The gram staining is done by first putting a sample of the unknown bacteria on a slide and it was heat fixed taking care not to over or under fixed the slide. The slide was flooded with crystal violet and let stand for 30
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Eubacteria - can be differentiated by Gram staining Gram positive: purple-blue‚ mostly peptidoglycan susceptible to penicillin Gram negative: pink-red‚ less peptidoglycan‚ has lipopolysaccharides‚ susceptible to EDTA Gram negative can be further differentiated into chlorophyllous (cyanobacteria) and achlorophyllous. Based on Gram staining Gram negative Escherichia coli Rhizobium Spirillum Pseudomonas Nostoc - cyanobacteria Oscillatoria - cyanobacteria Gram positive Staphylococcus aureus
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