CHAPTER ONE INTRODUCTION AND LITERATURE REVIEW 1.0 Ginger Ginger is a knotted‚ thick‚ beige underground stem (rhizome). The stem extends roughly 12 inches above ground with long‚ narrow‚ ribbed‚ green leaves‚ and white or yellowish-green flowers. The important active components of the ginger root are thought to be volatile oils and pungent phenol compounds (such as gingerols and shogaols). 1.1 Parasitological examination of stool specimen This is the examination of intestinal parasites
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Screening and identification of rhizobacteria isolated from heavy metal contaminated soils INTRODUCTION As a result of human activities such as mining‚ agriculture and industrial activity‚ heavy metal pollution has become one of the most serious environmental problems today. Elevated levels of heavy metals not only decrease soil microbial activity and crop production‚ but also threaten human health through the food chain. Soil microorganisms can degrade organic contaminants‚ while metals need
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Our main goal was to test organisms’ resistance and susceptibility to bacteria‚ and whether the cell wall of bacteria exposed the membrane to pathogen or protect it from pathogens. I thought P. aeruginosa was gram-positive and therefore it would be susceptible. That was my hypothesis.We divided antiseptic agar plate into quadrants and divided antibiotics agar plate into six areas. We wrote the organisms’ name‚ date‚ and the temperature on the bottom of the agar plates. For antiseptic‚ our group used
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Name Microbiology 2210 Answer Key Practice Test 1 Directions: Choose the most correct response to answer the statement. ___1. Archaea a. belong to the kingdom Protista b. have petidoglycan in their cell walls c. belong to the domain Bacteria
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colored colony. Gram-staining was utilized as a first step to differentiate between gram-positive and gram-negative. After determining that it was a Gram negative single bacillus‚ biochemical tests performed and their purpose and results are shown in table and flow chart form. Table 1: biochemical Test Results Test | Purpose | Reagents | Observations | Results | Gram stain | To determine the Gram reaction of the bacterium | Crystal violet‚ Iodine‚ Alcohol‚ Sarasin | Pink rod | Gram-negative rods
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Plasmodium‚ Trypanosoma Algae: Diatoms‚ Spirogyra‚ Volvox Helminths: Tapeworm‚ fluke Fungi: Methylene blue stain of yeast cells (Saccharomyces)‚ sporangiospores of Rhizopus‚ conidiospores of Penicillium Staining Labs (3-6‚ 3-7‚ 3-8‚ and 3-9) – Identify and correctly interpret the following: Lab 3-6: Gram stain Lab 3-7: Acid Fast stain Lab 3-8: Capsule stain Lab 3-9: Endospore stain Metabolic Tests: Lab 5-1: O-F Glucose (aerobic versus fermentative organisms) Lab 5-2: Phenol Red broth for sugar
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common between Archaea and Bacteria. In terms of their cell structures‚ Archaea are indistinguishable from Gram-positive bacteria. Within prokaryotes‚ only these two groups of organisms are bounded by a single unit lipid membrane‚ and they generally contain a thick sacculus of varying chemical composition. Some Archaea also show positive Gram staining and a few of them‚ similar to certain Gram-positive bacteria‚ do not contain any cell wall (Bacterial‚ 2006). The similarity between Archaea and Bacteria
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master my skills of using the Gram stain‚ testing for catalase‚ testing for coagulase and using the oxacillin screen. Now I am going to take you with me on the journey to the unknown. As I recall from earlier that semester‚ the first test that needed to be performed was the Gram stain. The Gram stain is a staining technique that aids in distinguishing cell wall characteristics and the cellular morphology. Bacteria will stain Gram positive‚ which is a purple color‚ or Gram negative‚ which is a pink
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procedure called gram stain is used in the identification of bacteria. Bacteria can be gram-positive‚ gram-negative‚ or gram-variable. Most bacteria however‚ are usually gram-positive or gram-negative. Gram-positive and gram-negative bacteria react differently to staining because of the differences in their cell walls. Bacterial cell walls contain a polymer called peptidoglycan. Gram-positive bacteria have more of this polymer than gram-negative bacteria. They are also less complex than gram-negative
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Abstract: It is important to be able to identify pathogenic bacteria that may be causing harm. Tomato crops can be affected by several different pathogenic bacteria. By using Koch’s postulates‚ it was determined that Pseudomonas syringae was the bacteria causing rot. There are four criteria that must be met when using Koch’s postulates. They are that the organism must be fund in all infected‚ the organism must be isolated in pure culture then once reinnoculated in a healthy host‚ must cause the
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