tube by running a series of experiments to detect which specific Gram negative organism we had. To detect your gram positive from the mixed culture was given as extra credit points also. A Gram stain was performed and isolation streak plate in order to isolate and observe the unknown organism. Before the series of test‚ a dichotomous key had to be written up in order to know what steps and tests to run to identify the unknown Gram negative organism. I had to run three experiments in all before I
Premium Bacteria Microbiology Staining
Tests done Test | Purpose | Reagents | Result (Positive) | Result (Negative) | Gram Stain | 1. To determine the cell wall formation ( thick layer peptidoglycan or thin layer peptidoglycan) | Crystal violet‚ iodine‚ 95% Alcohol‚ Safranin | Purple specimen- Gram Positive‚ cell wall has thick peptidoglycan layer | Pink Specimen- Gram Negative‚ cell wall has thin peptidoglycan layer | Catalase Test | To determine the production of catalase enzyme. The enzyme
Premium Bacteria Microbiology Agar plate
cause eye infections in men and women which can lead to blindness. It can also be passed from mom to baby during birth in the form of eye infections and pneumonia. HOW WAS IT DISCOVERED? c. Trachamotis was first discovered in 1907 by Giemsa staining by Halberstaedter and von Prowazek who took scrapings from individuals infected with trachoma and inoculated orangutans eyes with the samples. This demonstrated how the disease could be spread between infected individuals. In 1929‚ there was an
Premium Bacteria Cell
colony characterization and were estimated by their morphological and biochemical characters. As being a monsoon the occurrence of variation of different species were high. The microorganisms isolated from the soil were of staphylococcus strain and were gram positive‚ aerobic‚ coccus shaped bacteria. The fungal species were also identified‚ of which Aspergillus and Penicillium were dominant‚ followed by mucur‚ as sub dominant .This project aims to find out the water and soil quality of River and as it
Premium Soil Agriculture Water
REVIEW OF RELATED LITERATURE This investigatory project aims to make use of rambutan peeling extracts that is high in phenolic compounds such as tannin and saponin that exhibits antioxidant activity and antibacterial activity against pathogenic bacteria. Although the seeds contain such compounds the peeling exhibit greater amount of the said compounds. Rambutan is a very popular fruit cultivated in the Philippines and it is eaten in large amounts. The rambutans rind is quickly discarded and
Premium Bacteria Microbiology
(bacilli)‚ and spirals Diversity of prokaryotes Gram negative vs. gram positive bacteria (Fig. 27.3) Gram + vs - -: less peptidoglycan -: outer membrane -: lipopolysaccharides in outer membrane Why are gram– more pathogenic? Lipopolysaccharides are often toxic Outer membrane protects against the body’s defences Outer membrane impedes entry of antibiotics Common antibiotics inhibit cross-linking of peptidoglycan‚ particularly in gram+ Motility ? Most motile bacteria propel themselves
Premium Bacteria
impression that the litter level are the determinant of the cleanliness of the water. This study intends to examine: The bacteria population of samples obtained from several site across south east coast of England. Identify species of Gram Negative (-) & (+) of each sample. identify any faecal coliform bacteria which might suggest water contamination. These indicator organisms are used for indication of water contaminant by human and other warm-blooded animals (APHA 1992). Several
Premium Bacteria Microbiology Water
Generic name | Brand names | Common uses[2] | Possible side effects[2] | Mechanism of action | Aminoglycosides | Amikacin | Amikin | Infections caused by Gram-negative bacteria‚ such asEscherichia coli and Klebsiellaparticularly Pseudomonas aeruginosa. Effective against Aerobic bacteria (not obligate/facultative anaerobes) andtularemia. | * Hearing loss * Vertigo * Kidney damage | Binding to the bacterial 30S ribosomal subunit (some work by binding to the 50S subunit)‚ inhibiting the translocation
Premium Bacteria Cephalosporin Penicillin
appearance was observed and recorded. There was growth that appeared pinkish in color. The first test we did was basic stain using a heat fixed emulsion which kills the bacteria and allows them to adhere to the slide and thickens their protein for better staining. I then covered the heat fixed emulsion with crystal violet for one minute. The stain was then rinsed with distilled water. The stain was then pat dry with bibulous paper then observed under oil immersion to determine morphology. Upon completion
Premium Agar plate Bacteria Growth medium
Rocky Mountain Spotted Fever Rickettsia rickettsia was named after the first person who discover Rocky Mountain Spotted fever‚ Howard Taylor Ricketts. The unicellular aerobic gram negative non-motile‚ non-spore forming‚ coccobacilli bacterium are 0.3-0.5 x 0.8-2.0 um. It is in the family Rickettsiaceae and order Rickettsiales of the α-Proteobacteria. It is a unicellular microorganism that lacks a distinct nucleus and membrane- bound organelles‚ while it is a three-layered cell wall and its outer
Premium Bacteria Immune system Infection