Lambda DNA Amplification by Polymerase Chain Reaction (PCR) Introduction/ Background* Since its introduction in 1985‚ polymerase chain reaction (PCR) has become a powerful tool in molecular genetic analysis. Today‚ it is used for applications such as cloning‚ analysis of DNA from ancient specimens‚ and analysis of human DNA for forensic applications. PCR is a test-tube DNA replication system for making many‚ many copies of‚ or amplifying‚ a defined segment of DNA. Using PCR‚ a selected target
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Cause Bacterial meningitis can become serious even causing death in as little as a couple of hours. Those who are affected by this disease can recover for it. However‚ those who are unfortunate may have permanent disabilities including: hearing loss‚ brain damage‚ learning disabilities‚ etc. caused by the infection. There are a number of bacteria’s that result in meningitis such as: streptococcus pneumoniae‚ group B streptococcus‚ Neisseria meningitides‚ Haemophilus influenza‚ and listeria monocytogenes
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Diversity and Ubiquity of Microbes Pre-Laboratory Questions 1. What environmental settings would you expect to provide rich sources of microbes‚ in terms of overall numbers and different microbial types? Since the most of the microbes are ubiquitous‚ they are readily found in public bathrooms‚ phones‚ countertops‚ door handles‚ kitchen sinks‚ and human body parts such as the face and hands (Sherwood‚ 2017). In addition‚ public places exemplified by hospitals‚ grocery stores‚ and schools present an
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Sumon Mazumder Assistant Professor‚ Department of Textile Engineering Daffodil International University Shirajum Monira ID: 091-23-1405 Md.Rashedul Haque ID: 091-23-1413 M.K.Hasan ID: 091-23-1441 Level-4‚ Term-3 Department of Textile Engineering Daffodil International University Contents Topics Page Chapter: - 01 1.0 Aim of the Project…………………..………………………………....………….5 Chapter:
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LABORATORY EXERCISE 8-A: Preparation of Bacterial Smear and Simple Staining I. INTRODUCTION Bacterial smears are prepared for the purpose of viewing microorganisms under the microscope. Visualization of microorganisms in the living state is very difficult‚ not just because they are minute‚ but because they are transparent and almost colorless when suspended in an aqueous medium. A bacterial smear is a dried preparation of bacterial cells on a glass slide. Smears may be made from a dry culture
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of antibodies binding specifically to antigens in biological tissues.[1] IHC takes its name from the roots "immuno‚" in reference to antibodies used in the procedure‚ and "histo‚" meaning tissue (compare to immunocytochemistry). Immunohistochemical staining is widely used in the diagnosis of abnormal cells such as those found in cancerous tumors. A video showing preparation for immunohistochemistry (8 minutes) and then 20 minutes scanning through immunohistochemically stained tissues near colon cancers
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1. Colour Fastness to Light This method is intended for accessing the resistance of the colour of textiles to the action of daylight. Principle: A specimen of the textile is exposed to the light from a Xenon arc lamp under prescribed conditions‚ along with eight dyed wool standards. The fastness is assessed by compared the fading of the textile with that of the standards. Apparatus and Materials: - Blue standards - Humidity Test Control Fabric - Light fastness test apparatus containing
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definition of this group has been based on the method used for detection (lactose fermentation) rather than on the tenets of systematic bacteriology. Accordingly‚ when the fermentation technique is used‚ this group is defined as all facultative anaerobic‚ gram-negative‚ non-spore-forming‚ rod-shaped bacteria that ferment lactose with gas and acid formation within 48 h at 35°C. The standard test for the coliform group may be carried out either by the multiple-tube fermentation technique or presenceabsence
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HISTOPATHOLOGY: PAPANICOLAOU SMEAR PAPANICOLAOU SMEAR screening test for cervical cancer invented by George Papanicolaou(Father of Cytopathology)when he found out that cells in the cervix change before they become cancerous. recommended starting 21 y/o to 65 y/o Speculum – instrument used in the test Importance/Clinical Siginificance: For early detection of small tumors or pre-malignant cells in the cervix that may lead to cervical cancer. Specimen: cells in the cervix; the doctor or nurse starts
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of the activity. Record your observations. Part 2: Disinfecting Your Area to Use Live Organisms: Part 3: Viewing Live Organisms – Wet Mount Preparation Record your observations. Part 4: Direct Staining: Record your observations for each sample. Part 5: Indirect Staining: Examine the stained specimens and record your results. Questions: A. What are the advantages of using bleach as a disinfectant? The disadvantages? The advantages of using 70% alcohol? The disadvantages
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