observations. It was hard to tell what I was looking at. There were a number of amoeba shaped cells of varying sizes. There were five darker areas circular in nature. There were also three very large‚ odd shaped areas with distinct edges. Part 4: Direct Staining: Record your observations for each sample. Slide One: There were two distinct clusters that were easily noted. All the cells were cocci. Some of the cells were huge while others were practically nonexistent. Slide Two: There were layers of cells
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contain cocci with direct staining. At direct staining plaque smear contain cocci and bacillus. Yeast with indirect staining contains cocci as well. On same picture bacteria are singled while on some pictures bacteria are in group and uneven clusters. Different type of stain plays role to differentiate the shape of bacteria. C: When we are talking about difference between direct and indirect stains‚ main importance is acidity of the dye. Dye that we are using in direct staining is basic. The colored
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FOOD AND INDUSTRIAL MICROBIOLOGY Food spoilage‚ food infections and intoxications caused by microorganisms and methods for their detection Dr. Neeraj Dilbaghi Reader‚ Department of Bio & Nano Technology Guru Jambheshwar University of Science and Technology Hisar- 125001 and Dr (Mrs.) S. Sharma Professor‚ Department of Microbiology‚ CCS Haryana Agricultural University‚ Hisar- 125001 (Revised 25-Sep-2007) CONTENTS Introduction Food Spoilage and General Principles Underlying Spoilage of Food Intrinsic
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Chapter 17 Louis Pasteur’s experiment illustrated that: microbes will not grow in a nutrient broth that has been sterilized unless air is allowed to enter the vessel through an opening. Living organisms can spontaneously generate from nonliving matter. True or False? False The scientists usually given credit for disproving the theory of spontaneous generation of bacteria are: Tyndall & Pasteur Miller and Urey’s experiments attempting to recreate the prebiotic environment produced a rich mixture
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2.4 Mechanism Of Phosphate Solubilization: In contrary to Nitrogen soluble Phosphorous is not supplied to plants by atmosphere . Due to this reason the primary and secondary minerals and/or organic compounds are the source of P to great extent. In soil solution concentration of phosphorous is least as compare to other nutrients and ranges from0.001 to 1mg/ml Brady and Weil (2002 ).There are widely three categories of P compounds in soil: (i) compounds which are inorganic‚ (ii) organic compounds
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Quantitative histological analysis of the laceration site by vimentin immunohistochemical staining 4 weeks after injury. Top panels (A)‚ vimentin immunohistochemical stain; lower panel (B)‚ area of scar tissue per field. Control (C)‚ muscles treated with IGF-1 (IG)‚ muscles treated with decorin (D)‚ and muscles treated with both agents (IGϩD); *P Ͻ 0.05. FIGURE 3. Quantitative analysis of scar tissue by trichrome staining. Scar tissue containing collagen (blue) was analyzed. Top panels‚ trichrome stain;
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Genetically Modified Organisms INTRODUCTION: The purpose of this lab was to identify if non-labeled food products are actually genetically modified foods. Before we could begin testing this theory we first had to gain an understanding about genetically modified organisms in general. This was rather easy because if you have been to any grocery store lately you have without a doubt seen products with labels saying "GMO-free" or even "contains only non-GMO ingredients." GMO actually stands for
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arising from the isthmus in front of the larynx • The gland is well vascularized‚ and the thyroid has one of the highest rates of blood flow per gram of tissue of any organ in the body. Anatomic considerations • The thyroid is made up of multiple acini (follicles). • Each spherical follicle is surrounded by a single layer of cells and filled with pink-staining proteinaceous material called colloid. Anatomic considerations • When the gland is inactive‚ the colloid is abundant‚ the follicles are large
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by Bacillus sp.: The microorganisms in the most contaminated toilet Sink were isolated by dilution technique. The dominant bacterial strains are identified as Bacillus sp.‚ on the basis of morphological and biochemical characteristics. Gram’s staining: Gram positive and rod shape. They were serially diluted and spread on nutrient agar plates. The starch has been hydrolyzed and a clear zone by the addition of iodine solution. Visual identification: When bacterial biomass mixed with silver nitrate
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rapid succession to heat fix the smear. Simple Staining = staining cells with a single dye so that they can be more readily observed. Basic Dye = (methylene blue) has positively charged chromophore group. A basic dye will be attracted to any negatively charged substance‚ such as bacterium. Acidic Dye = (eosin) have negatively charged chromophore groups‚ and thus are attracted to positively charged substances. Chromophore Group = chemical staining group. Bacilli = rod- shaped bacterium. Endospore
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