CHAPTER I INTRODUCTION Pneumonia is one of the most common infectious diseases prevalent nowadays and affects all ages. It is an acute or chronic infection of one or both lungs caused by microorganisms‚ such as viruses‚ bacteria or chemical irritants. (Schmitt‚ 2011) It has different types‚ and one of them is Community Acquired Pneumonia (CAP). CAP is a disease in which individuals who have not recently been hospitalized develop an infection of the lungs. It occurs because the areas of the lung
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‘slice extremely thin sections for viewing under the microscopes’ as Robert Hooke found that cutting a cork in a thin section gets a clearer view. As technological advances advanced new techniques also developed over time for example staining techniques. Staining techniques was developed to view and understand tissues under the microscope by using dyes to stain tissue and visualise
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Microorganisms are classified according to their structure. By means of flow charts‚ diagrams and tables explain the differences between Viruses‚ Bacteria‚ Cyanobacteria‚ Achaea and Fungi. Bacteria or bacterium are unicellular microorganisms. They are essentially only a few micrometres long and form of various shapes including the spheres‚ rods and spirals. A BACTERIAL CELL Illustration courtesy of Wikipedia. A Virus (from the Latin noun virus‚ meaning toxic or poison) is a sub-microscopic
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1. The final yield of the cells can be determined by use of an automated cell counter or by use of a hemocytometer (Neubauer chamber). In our laboratory‚ we determine the number of purified cells in a Neubauer-improved‚ bright line chamber with V-slash with a depth of 0.1 mm and a counting area of 0.0025 mm2 from Marienfeld (Lauda Königshofen‚ Germany‚ PN # 0650030). 2. For determination of the final cell yield‚ mix a 10 µl aliquot of the purified cells (Figure 9D) with 10 µl of trypan blue solution
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of a pathologist‚ assisting in different medical laboratory examinations. The medical examinations may include but are not limited to surgical procedures during autopsies‚ carefully analyzing of surgically removed tissues as well as preparing and staining of those tissues for further examinations in order to assist in the diagnosis of the pathologic conditions or death. Pathologists’ Assistants also assist in administrative duties such as maintaining of protocols related to pathology procedures. I
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Bradford and Ghosh/Dumbroff methods were used to calculate the amount of the dissolved proteins in the solvent. SDS-PAGE electrophoresis was used to separate the polypeptides in the mixture and was visualized by coomassie brilliant blue and silver staining. Western blot method was used to detect the molecular weight of proteins which is selected for three different antibodies: α-β-actin targeting the α and β isoforms of actin protein‚ α-GADPH targeting Glyceraldehyde 3-phosphate dehydrogenase‚ (GAPDH);
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Over the past four years‚ the rate of autism diagnosed has increased over 30%. Autism spectrum disorder is a neurodevelopmental disorder in which the diagnosed exhibit social and behavioral deficits. Since autism is a life-long impairment‚ researchers are attempting to rapidly find the causes in the hope to find a cure. It is theorized that prenatal hormone exposure could potentially result in ASD. Therefore‚ the prenatal hormones estradiol and dihydrotestosterone were subcutaneously injected into
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Moreover‚ the technological introduction of stains were developed and assisted scientists to efficiently see cells and their internal structures. This staining technique‚ as a result of technology‚ created a contrast between the transparent material and its background‚ presenting a clear image of the cell. Therefore‚ through the technology advancement in staining techniques‚ the processes of the cells and nuclear division of the cell were visible. Significantly‚ towards the end of the 19th century‚ compound
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you select a palette for the exterior of your home‚ remember that you should be complementing your entire property. The team at Curragh Painting can help you with a deck staining to make sure that your wood finish matches your new palette. But things like driveways and established landscaping are harder to tackle than a deck staining‚ so think about what you can and can’t easily switch up before deciding on a new palette.
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Inspecting Plumbing Plumbing problems always make themselves known. The main question for a homeowner is if the problem is discovered early or late. Early discovery lead to the easiest repair‚ least expense‚ and least life disruption. A routine inspection plan by the homeowner can pay in money and in hassle. Initial Inspection A good home inspector will look at plumbing and note obvious visible issues. For older home or home in older neighborhoods‚ consider using a professional plumber to inspect
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