Characterization of a Yam Class IV Chitinase Produced by the Recombinant Pichia pastoris X-33 Department of Botany‚ Jahangirnagar University‚ Savar‚ Dhaka 1342‚ Bangladesh Abbreviations: Endo H‚ Endoglycosidase H; PR protein‚ pathogenesis related protein; P. pastoris‚ Pichia pastoris; VTS‚ vacuolar targeting signal; PCR‚ polymerase chain reaction; PAGE‚ polyacrylamide gel electrophoresis; SDS‚ sodium dodecyl sulfate Abstract A yam (Dioscorea opposita Thunb) class IV chitinase‚ whose genomic
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Evolution as a theory * Theory- in science‚ a well substantiated explanation of some aspect of the natural world Evolution- the accumulation of inherited genetic changes within populations over generations * Does not refer to changes that occur in an individual within its lifetime * Not limited to speciation that may only occur over long periods of time * Can occur over a few generations‚ and is most commonly expressed as an adaptation to a changing environment or the change in allele
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1:500‚ 1:1000‚ 1:2000‚ 1:8000) during 10 minutes. The increasing molar ratios were assessed by gel retardation assay on a 0.8% agarose gel and visualized by ethidium bromide staining. For further experiments‚ all samples were prepared in the 1:8000 pDNA:protein molar ratio with 1
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Miranda Miller Jackie Farmer Integrated Reading/Writing II 27 October 2014 Wasting Away Pound by Pound A big issue in this society today is eating disorders. According to NEDA‚ National Eating Disorder Association‚ 20 million women and 10 million men in the United States suffer from eating disorders including anorexia nervosa‚ bulimia nervosa‚ binge eating disorder‚ or an eating disorder that has not yet been specified. Also‚ by age six most girls start to express concerns over their weight
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Autoradiography Presenter: Miranda Grounds References: Alberts‚ p174-176‚ 730. Rogers A (1979) Techniques of autoradiography. 3rd Edition. Elsevier‚ North Holland pp429. Radiography is the visualisation of the pattern of distribution of radiation. In general‚ the radiation consists of X-rays‚ gamma (g ) or beta (b ) rays‚ and the recording medium is a photographic film. For classical X-rays‚ the specimen to be examined is placed between the source of radiation and the film‚ and the absorption
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cis-Golgi network ‚medal-Golgi‚ endo-golgi‚ and trans-Golgi network. Vesicles from the endoplasmic reticulum. Location of Golgi Apparatus in cell. Working of Golgi Apparatus. Techniques used to study the Golgi Apparatus. • Cell Staining: Fluorescent Labelling of the Golgi Apparatus‚ Immunofluorescence can be used to visualize proteins in the Golgi apparatus or its subdomains. • Immunofluorescence has been the primary method for labelling the Golgi apparatus for light microscopic
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EXPERIMENT NO. 15 PROTEIN CHARACTERIZATION BY ELECTROPHORESIS Abstract The molecular weights of protein extracts were assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Two sets of four protein samples‚ standard bovine serum albumin (BSA)‚ invertase‚ egg albumin‚ and casein‚ were prepared; one set containing β-mercaptoethanol (BME) while the other did not. These were then analyzed through SDS-PAGE with 12.5% resolving gel‚ prepared using 2 M Tris-HCl at pH 8
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ABSTRACT The title of our project was Bilimbi fruit as an alternative stain remover. The purpose of this experiment was to find out if Bilimbi can remove stains from our clothes. We all know that teenagers today are very eligible especially in wearing dress. We conduct this kind of study to be able to have another background study about natural bleaching agent and to build natural product that is a solution for a stains The procedure involved was to apply the extract of Bilimbi to the clothes
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significantly after which they can be seen under the light microscope as distinct thread-like structures. Chromosomes are usually studied by obtaining actively dividing tissue‚ fixing the material to kill the cells‚ and to preserve their structure‚ staining the chromosomes with a dye‚ and then observing them with a microscope. By studying mitosis in this way‚ changes that occur in the chromosomes can be observed and the visible events that bring about the process of heredity in somatic (body) cells
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conversation with miss Qutachi‚ I found that she has not made new friends in the city. On exam‚ I found a few cavities in her molars and generalized staining on her teeth. I asked her if she has any idea on why she developed stains on teeth. I allowed her to led the discussion on stains and she pointed out to coffee and smoking habit as potential reasons for the staining. Once she started talking about smoking‚ I asked her permission to collect some information on smoking history. I learned that she has been
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