Objectives Protein Isolation: Protein isolation for a western blot uses detergents and mechanical force to separate seeded cells from their container. Eukaryotic cells are attached to the surface of a flask by cadherins. In the past‚ we’ve separated the cells from the flask by breaking these bonds with a protease‚ but in order to keep the proteins intact‚ a different method needs to be used to extract the proteins. In protein extraction for a western blot‚ we use detergents to lyse the membrane
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NAME: Donna Ha TITLE: Measuring the Rate of Osmosis Using “De-shelled” Chicken Eggs INTRODUCTION: In order to fully understand the concept of this experiment‚ one must first be introduced to diffusion. Diffusion is a spontaneous process by which molecule particles move from one area that is highly concentrated to another area in which its concentration is lower. Cells contain fluids and are surrounded by fluids; in order for a cell to function it is required to be in a balanced state. The
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Chloride is produced? Hypothesis: If there is one mole of BaCl2 in the reaction‚ then two moles of AgCl will be produced. Variables: Independent: Amount of the compound‚ BaCl2‚ in grams. Dependent: Amount product produced‚ AgCl‚ in grams. Materials: * 4 -grams of Barium Chloride (BaCl2) * 8 -grams of Silver Nitrate (AgNO3) * Analytical Balance * 2- Volumetric Flasks; one 50 mL flask‚ and one 100 mL flask * 2- Scoopulas * 10- Weigh Boats * 5- Filter paper *
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report discusses an experiment that was done to demonstrate the effects of lysozyme on populations of Gram positive and Gram negative bacteria. Bacteria have a cell wall composed of peptidoglycan that gives the wall its strength. Gram negative bacteria have and extra component of lipopolysaccharide (LPS)‚ that is stabilized with magnesium ions‚ to their cell wall that further protects them. When Gram positive bacteria are treated with lysozyme‚ the lysozyme breaks down the peptidoglycan‚ allowing the
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loss of potassium‚ sodium and chloride from the body as a result of purging behaviours. • Potential for gastric rupture during periods of bingeing. • Inflammation and possible rupture of the oesophagus from frequent vomiting. • Tooth decay and staining from stomach acids released during frequent vomiting. • Chronic irregular bowel movements and constipation as a result of laxative abuse. • Peptic ulcers and pancreatitis Health Consequences of Anorexia Nervosa In anorexia nervosa’s cycle of
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In today’s society where everyone is always busy and on the go‚ fast food is inevitable‚ but it does not have to be a complete disaster concerning nutrition. When I consider the taste of McDonald’s compared to the taste Of Burger King‚ I prefer Burger King. I have always been curious about the nutritional value of some of these items‚ but always worried I was making the wrong choices. I started by noticing the toppings and servings of a few of my personal favorite menu items. After looking at
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tissues were fixed in neutral buffered formalin and embedded in paraffin‚ sectioned at 5 μm and subsequently stained with hematoxylin/eosin to see the architecture of hepatic tissue and inflammatory cell infiltration. Sirius red staining for fibrosis and Prussian blue staining for iron deposition were also done in liver sections. Sections were then studied and photographed under light microscope (Zeiss Axioscope) at 40 magnifications. Collagen deposition was semi quantitatively measured using NIH Image
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ID130 Week 3 Assignment 1 Transforming Surface Appearance Embellishing Fabric Surfaces - the purpose of this technique is to create items such as needlepoint pictures‚ quilts‚ doilies‚ crewel pillow covering‚ and soft sculptures on curtain fabrics or tabletop accessories (Textiles). This technique is accomplished by many techniques such is needle point stitching also referred to as canvas embroidering which is where yarn is used to cover all or part of the fabric. Improving the Quality and Serviceability
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FST3202 Food Microbiology 2012/2013-1 Lecture 1. Introduction to Food Microbiology Prof. Madya Dr. Yaya Rukayadi Department of Food Science Faculty of Food Science and Technology Universiti Putra Malaysia Wednesday‚ 12-09-2012 BKTM-2 (10.00 – 12.00) FST 3202 – Food Microbiology Semester : 1 (2012/2013) Program : Undergraduate Student (prasiswazah) Credits : 3 (2-1) Class : BKTM 2 – Food 1 Day/Time : Wednesday‚ 10.00 – 12.00 Pensyarah : Prof. Madya
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Exercise 8-3 Direct Materials Budget Three grams of musk oil are required for each bottle of Mink Caress‚ a very popular perfume made by a small company in western Siberia. The cost of the musk oil is $1.50 per gram. Budgeted production of Mink Caress is given below by quarters for Year 2 and for the first quarter of Year 3: Year 2 Year 3 First Second Third Fourth First Budgeted production‚ in bottles 60‚000 90‚000 150‚000 100‚000 70‚000 Musk oil has become so popular as a perfume ingredient
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