As mentioned above‚ bacterial growth rates during the phase of exponential growth‚ under standard nutritional conditions (culture medium‚ temperature‚ pH‚ etc.)‚ define the bacterium’s generation time. Generation times for bacteria vary from about 12 minutes to 24 hours or more. The generation time for E. coli in the laboratory is 15-20 minutes‚ but in the intestinal tract‚ the coliform’s generation time is estimated to be 12-24 hours. For most known bacteria that can be cultured‚ generation times
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Endospore and Capsule Cell Staining Allison Lui Partner: Mary Chagin BIOL-235 W07 Dr. Runco 2/10/15 Introduction The purpose of this lab is to learn what endospore and capsules are‚ and how to identify them under a microscope using capsule and endospore staining methods. Capsules are found only in select bacteria‚ and serve a protective purpose. Made out of sugars and proteins‚ they are antiphagocytic‚ which prevents other cells to engulf the bacteria through phagocytosis. It
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The methylene blue staining procedure is used to measure yeast viability based on the assumption that the methylene blue will enter the cells and be broken down by living yeast cells that produce the enzymes which breaks down methylene blue‚ leaving the cells colourless. The non- viable cells do not produce this enzyme (or enzymes) and as such the methylene blue that enters the cells are undegraded causing the cells to remain coloured (the oxidized form concentrates intracellularly). The coloured
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Microbiology 197 Prepared Bacteria Gram Stains (F12) Materials required: * Microscope; clean and properly set up * Immersion oil * Lens paper * Lens cleaning fluid * Microscope drawing forms * Specimens: 1. Bacillus subtilis 2. Staphylococcus aureus. 3. Escherichia coli Procedure: 1. Observe each of slides listed in “Specimens” above. 2. Make your observations using oil immersion (1000X). 3. Using a drawing form draw the organisms
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Ever get "subject/verb agreement" as an error on a paper? This handout will help you understand this common grammar problem. Making Subjects and Verbs Agree 1. When the subject of a sentence is composed of two or more nouns or pronouns connected by and‚ use a plural verb. She and her friends are at the fair. 2. When two or more singular nouns or pronouns are connected by or or nor‚ use a singular verb. The book or the pen is in the drawer. 3. When a compound subject contains both a singular
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culture‚ I immediately made a streak plate and started the Gram Stain protocol1. In the first two slides‚ I made and stained‚ my results were ambiguous. Without putting the slide into the microscope‚ I noticed one was pink and the other purple. To resolve it‚ I discarded my slides and started again and was extremely careful in proceeding the Gram Stain. I concluded that my unknown A was gram-negative bacilli. Using the dichotomous key for gram-negative bacilli I started the protocol for the lactose
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Judson Cotterman III 4/6/2012 Section 185-35L Assignment #3 Gram-positive Pathogenic Cocci Discussion My original hypothesis for the individually collected throat culture on Blood Agar Plates‚ that it would show a mixture of alpha‚ beta‚ and gamma hemolysis‚ was based on the results of the class control samples of S. pneumonia‚ S. aureas and S. epidermis. In these controls S. pneumonia shows alpha and beta hemolysis while S. aureas and S. epidermis showed beta and gamma hemolysis respectively.
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1. What are the advantages and disadvantages of the different types of light and electron microscopes discussed in Chapter 3 that are used to study microorganisms? Focus your response in terms of the following parameters The disadvantage of using a light microscope is its inability to magnify a specimen as great as an electron microscope. An electron microscope can magnify up to 500‚000 times‚ whereas‚ a light microscope can only magnify an object up to 2000 times. The resolving power or resolving
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agency NASA selected Gram Power’s technology among the top-10 cleantech innovations from around the world. Two fresh graduates from the University of California Yashraj Khaitan and Jacob Dickinson founded GRAM POWER which enables villagers to generate and store renewable energy from biomass‚ solar or wind on-site. While on a university project they came to know about the scarcity of electricity in villages‚ this goaded them to devise energy solutions for villagers. This year‚ Gram Power set up India’s
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Microscopy and Staining Abstract The primary focus of this lab was on microscopy and simple stains. Microscopy that was used were magnification‚ slide preparation‚ and staining. Methylene blue‚ a simple staining component‚ was used to stain the slide in order to see the different microbes and determine their cellular shape. Introduction The purpose of this lab was to become familiar with the light microscope and how to accurately use it to view specimens. The staining method was used
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