The conventional method was conducted by placing 2.0641 grams of salicylic acid in a dry 125 mL Erlenmeyer flask. In a fume hood‚ 4 mL of acetic anhydride and 5 drops of concentrated sulfuric acid were added to the flask. The contents were swirled in the flask and then heated for 20 minutes in a hot water bath. After the 20 minutes were up‚ 10 mL of cold water were added in 1 mL increments. The flask was cooled for 10 minutes and occasionally stirred. The crystals that formed were collected via filtration
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Method • Methodology is performed through 3 successive days: First Day: Trypan Blue cell viability assay: Description Trypan blue staining is a simple way to evaluate cell membrane integrity (and thus assume cell proliferation or death) but the method is not sensitive and cannot be adapted for high through put screening. Short 96 well assay: EACH condition should be done in triplicate or more. Steps 1. Trypsinize one T-25 flask and add 5 ml of complete media to trypsinized cells. Centrifuge
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MICROBIOLOGY 3444-007 Gram Negative Unknown Unknown Bacteria #14 LeNaiya Kydd 4/2/2014 Abstract In order to be able to identify the unknown organism that was given to us‚ we had to conduct a number of different tests. These biological tests are used because they help us be able to identify the properties of the unknown we have and be able to compare our observed results with actual results of all the potential organisms. When all of the data of the test are put together it is easy to
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Lab 5 –Cell Structure and Staining using Microscopy Instructions: Please download this MSWord document to your computer and answer the questions as asked. Then save the document and upload it to Bb using the Assignment feature provided. This assignment is worth a total of 100 points – there are 20 questions worth 5 points each. NAME Buket Rembert In Lab 3 you were introduced to microscopy. In this lab you will be adding to that experience by reviewing the differences in cell structure for Prokaryotes
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(2005) defined staining as the method of artificially producing color in microorganisms to allow for the visualization under the microscope. He further stated that stains are used not only to make the organisms visible but also to understand their structure and chemical nature. Gram staining is a technique commonly used in the laboratory. It is used to differentiate two large groups of bacteria based on their different cell wall constituents. It distinguishes between Gram positive and Gram negative groups
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In addition‚ Gram and Gramps also helped Sal accept her mother’s death. Within the book‚ on page 143 Gram says to Sal‚ “Sometimes you know in your heart you love someone‚ but you have to go away before your head can figure it out” (Creech 143). I can infer‚ that Gram was trying to explain to Sal that her mother didn’t leave because of her‚ but to clear her mind. Gram saying this to Sal was important for Sal’s knowing that her mother didn’t leave because of the baby that died before birth. Along with
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Blood Film Staining – Normal & Malignant Cells Case Study 1 Male 72 yrs Hb 77g/l (NR 130-180) RBC 2.23 x 10¹²/l (NR 4.5-6.5) MCV 88fl (NR 76-96) WBC 98.2 x 10/l (NR 4-11) Platelets 28 x 10/l (NR 150-400) A) Calculation of PCV (packed cell volume or haematocrit) PCV = RBC (cells/l) x 10¯¹² x MCV (fl) RBC = 2.23 x 10¹²/l MCV = 88fl or 88 x 10/l PCV = 2.23 x 88 x10 PCV = 196.24/1000 PCV = 0.196 (NR 0.4-0.54) B) Calculation of MCH (mean cell volume) MCH = Hb
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fountain was rising in popularity as a social gathering spot.And this was when Coca-Cola was born. Now coke refreshes many people’s mouths as one of the most popular drinks in 2016. To continue‚ one of the primary drawbacks to drinking tea is the staining effect it can have on your teeth. Tea contains a substance called tannic acid‚ which is what gives it the dark color‚the tannic acid may settle into your teeth‚ causing
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Bio Lab Report Title: Gram-Negative and Gram-Positive contamination in yogurt. Introduction: There is bacteria that is beneficial and harmful in the human. Yogurt was choosed to be the best example that contains this bacteria. What happens to be the difference between these two types of bacteria‚ and how are they harmful? The bacteria is considered “Gram-Negative‚” and the substance is considered contaminated when there is more negative bacteria than positive bacteria. This will prove or disapprove
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Different kinds of liquids stain your teeth differently. For example tea stains your teeth more than milk does. I’m doing this experiment because I think it is very interesting that there are so many different kinds of liquid that can stain your teeth. What things stain your teeth? To start with you should never start smoking or use tobacco. The nicotine and tar in the cigarettes stain your teeth very drastically. You can’t even imagine how fast smoking stains your teeth. Other things that stain
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