1. Make sure you label both plates on the bottom‚ with your name‚ date and name of pathogen you have chosen (listed above under materials) Then label numbers 1-5 on one plate and 6-10 on the other as there are 10 disks that need to be placed. Spread out in a circle formation like pictured below (This is the final product; look at this for reference on how to label) Now you are ready to start culturing. Start with one of your plates you can do both at the same time but to avoid making any errors
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Lakeli is absolutely stunning‚ but for his beauty he has paid the price. He is a beautiful 12 week old deaf Great Dane. While we all think white Great Danes are stunning most of them are going to be either blind‚ deaf‚ and occasionally both. What is a Double Merle and how are they created? A few things to keep in mind about the Merle pattern as you read. •Merle is a pattern not a color. •Merle is a bleaching pattern. •The Merle gene creates mottled patches of color in a solid color coat‚
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bacteria E.coli was the only type found in the given area by gram staining. E.coli was the chosen bacteria for this type of experiment. It is a gram negative bacterium that will grow rapidly given ‘any culture medium with the necessary energy source‚ nutrients‚ pH‚ and temperature’. Therefore‚ MacConkey Agar being the medium for its growth will enable us to achieve the experiment outcome. MacConkey Agar only allows the growth of gram negative bacteria as they do not contain bile salts and crystal
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Escherichia coli is a rod-shaped Gram-negative bacteria from the Enterobacteriaceae family‚ which owns the usual flora and can be be as pathogenic Human IN DUE can be cause diarrhea. The rational NOT USE ON antibiotics Infectious diseases can be causing the occurrence of resistance so that the necessary alternative to using Active Ingredients antimicrobial From medicinal plants. Turmeric (Curcuma domestica Val.) It is a prayer One Crop Growing in Indonesia That can be used as a medicinal plant. BECAUSE
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provide accurate data. 2. Through this letter I would like to present my opinion regarding Grams staining method and reliability of this method. According to me‚ Grams staining process is a simple technique that assists in recognition of etiological agent and therefore can be called as one of the most significant staining practices in microbiology. Though‚ Gram is concerned that the staining process developed by him is imperfect because not all bacteria can be stained by it‚ but in my opinion
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known that were mentioned above. First it was the Gram staining procedure used to categorize bacteria in two groups‚ gram positive and gram negative. Gram staining is one of the most useful test used in the clinical setting to identify bacterial colonies due to their broad staining spectrum. (Black‚ 2008‚ pp. 70-71) The basis of the Gram stain is that gram positive bacteria retains the color of the primary dye‚ the crystal violet‚ whereas the gram negative bacteria loses the primary dye once its
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ATM (STP). A Gram stain was then carried out to differentiate the unknown sample from a broad class to a more specific category of bacteria. The Gram stain distinguishes between Gram-positive and Gram-negative bacteria based on the composition of the cell walls. Gram-positive bacteria appear purple and Gramnegative bacteria appear pink after staining. The first Gram stain produced unsatisfactory results and was then repeated with a clear indication of negativity. Light pink staining was evident
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pathogens and their related epidemiology has become increasingly more important. The purpose of this study was to identify an unknown bacterium in a controlled laboratory environment over a 5 week period. Utilizing a variety of differential testing and staining methods learned in the microbiology course‚ students were to determine the identity of an assigned unknown organism. Observations were made and recorded each week to narrow down the scope of identification. Data has been presented in the tables
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Sarcina lutea‚ Pseudomonas fragi‚ Micrococcus luteus‚ Alcaligenes faecalis‚ Clostridium sporogenes‚ and Micrococcus roseus. There were several qualitative tests that could be conducted to determine the identity of the unknown species‚ for example‚ Gram staining‚ Fermentation‚ Catalase‚ Oxidase‚ Starch Hydrolysis‚ Litmus Milk‚ MOI medium‚ and the Gelatin Test. All tests and techniques used were performed in accordance with The Microbiology Lab Manual. Materials and Method: A stock broth culture of
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MacConkey Agar test This test is used for isolating the lactose fermenting bacteria from lactose non-fermenting bacteria while inhibiting the growth of gram positive bacteria. The lack of growth from my bacteria indicates that my bacterium has negative result. Mannitol Salt Agar This test is used to observe the difference between gram positive staphylococci bacteria and to differentiate for mannitol fermentation. If a yellow media change is observed then the bacteria has the presence of
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