contaminated dirt by running it through a series of identification techniques. Some of these techniques were simple staining‚ gram staining‚ and isolation techniques. The hypothesis of this experiment was that the bacterium is of the bacillus family due to the rod shape. Due to multiple complications with the PCR‚ a new hypothesis couldn’t be formulated. Methods: In this experiment‚ a 1 gram sample of contaminated dirt was taken and mixed with 100 mL of sterile water then shaken. After allowing the sediment
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Chapter 3 Staining Lab Quiz Section: Multiple Choice 1. Which of the following stains is used frequently to identify Mycobacterium and other bacteria whose cell walls contain high amounts of lipids? A. Gram stain B. Schaeffer-Fulton stain C. Acid-fast stain D. Lipidialar stain E. Spore Stain 2. Which of the following stains is used to classify microorganisms based on their cell wall content? A. Capsular stain B. Gram stain C. Spore stain D. Negative stain E. Methylene blue 3. In the Gram stain
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hyphal cells. d. True hyphae- Fundamental microscopic units of fungus‚ tube-like projections with no constictions at the cell wall. The cell walls remain parallel with no indentation. 3. Describe the appropriate specimen collection procedures‚ staining methods‚ and culture techniques used for isolation of yeast. Collection procedures Specimen of choice include respiratory secretions‚ hair‚ skin‚ nails‚ tissue blood or bone marrow and CSF. Swabs are inadequate. CSF: Concentrate by centrifugation
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PSBPP103 Differential Gram’s staining EXPERIMENT NO. 1 AIM: THEORY: Page No: C1 Date: To Gram stain the given bacterial suspension and to differentiate between gram positive and gram negative organism. Visualization of microorganisms in the living state is very difficult‚ not just because they are minute‚ but because they are transparent and almost colorless when suspended in an aqueous medium. To study their properties and divide microorganisms into specific groups for diagnostic purposes
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Review all chapters in your exercise manual beginning with the introduction INTRODUCTION: (Covered 9.4.14 II Week 1) Biosafety levels1: basic level of containment. Hand washing or wearing gloves 2: Appropriate for working with human body fluids. Autoclave‚ sharps containers‚ lab coats 3: appropriate for working with pathogens that can be transmitted via respiratory route. Self-closing‚ double doors and sealed windows 4: Highest level. Aerosol pathogens; pathogens with no vaccine/treatment. Separate
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Differential Staining Purpose: The purpose of this experiment was to become familiar with subtypes of culture media and the uses for each‚ learn and employ the streak and pour dish techniques‚ and generate a pure culture of a specific organism. Set Up: For this experiment I needed: 1 Distilled water‚ 1 Paper towels‚ 1 10%-bleach or 70% alcohol solution‚ 1 Zip bag‚ 1 Pan to heat agar‚ 1 Isopropyl alcohol (rubbing alcohol)‚ 1 Cultures: S. epidermidis and L. acidophilus‚ 1 Gloves‚ Disposable
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Week 1 Chapter 3 Staining Lab Quiz SCI/250 SCI250 Week 1 Chapter 3 Staining Lab Quiz (6.7 points each) Check or underline the correct answer Section: Multiple Choice 1. Which of the following stains is used frequently to identify Mycobacterium and other bacteria whose cell walls contain high amounts of lipids? Gram stain Schaeffer-Fulton stain Acid-fast stain Lipid stain Spore Stain 2. Which of the following stains is used to classify microorganisms based on their cell wall
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any two (2) microorganisms that are not classified as prokaryotes are and The two (2) genera of bacteria that are capable of producing spores are and b) c) d) An example of a Gram positive bacteria is example of a Gram negative bacteria. and is an e) Three (3) common differential staining methods used in microbiology are ‚ and "Ubiquitous" in microbiology means The minimum inhibitory concentration (MIC) means Of more than three thousand (3000) species of bacteria‚ only 10%
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sample was taken from a door handle to the George Lynn Cross Hall‚ which is touched by hundreds of students entering the building every day. The most important exercises performed include gram staining‚ which
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harvard.edu/node/222 BSP‚ S. (2010). How is EM different from light microscopy? Retrieved April 25‚ 2015‚ from http://bsp.med.harvard.edu/node/222 Tortora‚ G.‚ Funke‚ B.‚ & Case‚ C. (2010). Staining Techniques. Retrieved April 25‚ 2015‚ from http://www.cliffsnotes.com/sciences/biology/microbiology/microscopy/staining-techniques
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