The pGLO lab is a lab where students attempt to put the genes that make a jelly fish glow into E. Coli. After a process called transformation‚ the process in which a cell takes up and expresses a new piece of genetic information‚ the E. Coli will be able to glow and will be antibiotic resistant. The students first need to learn a couple of techniques before they are able to begin this lab. The first technique they will need is how to keep their environment sterile. They must learn to only open their
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Creature one was removed from the package then and weighed in grams‚ as well as creature two. The creatures were also measured in millimeters. The first creature resulted in thirty millimeters and one gram‚ and the second creature resulted in thirty-two millimeters and one gram as well. Number Creature 1 Creature 2 Length (mm) 30mm 32mm Mass (g) 1.0g 1.0g Below‚ the table shows the data collected after the creatures
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lab measurements must be wrong because at 4ºc‚ water has a density at 1. The colder the water‚ the higher the density. 2. For the aluminum‚ I got 2.50 g/mL . The discrepancies may be that the literature value measurements measure at a different gram or mL‚ also because the aluminum may not has been torn off evenly. Although the measure 2.50 g/mL as a density in my calculations was very close to the literature value of density of 2.70 g/mL‚ there is only few explanations why the calculations would
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below. Figure 1. Apparatus We followed this procedure for finding the percent of water in bluestone: 1) Mass a massing tin on a hanging pan balance‚ record mass‚ and then add four grams of crushed bluestone to the massing tin‚ record mass. 2) Carefully pour bluestone from massing tin to crucible. 3) Set up your apparatus as shown in Figure 1. 4) Light the alcohol burner and heat until the bluestone turns from blue to white
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all other components the same. Apparatus Material | Uncertainty | Quantity | Different spaghetti packets with differing carbohydrate content | n/a | 5 | Retort stand | n/a | 1 | Sticky tape | n/a | 1 | Meter ruler | ±0.05cm | 1 | 10 gram weights | ±0.5g | 20 | Clamp | n/a | 1 | Sticky Tape Edge of a Table
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Experiment 2: Microscopy & Simple Staining September 30‚ 2013 General microbiology laboratory The invention of the microscope has opened up a whole new dimension in science. By using microscopes scientists were able to discover the existence of microorganisms‚ study the structure of cells‚ and see the smallest parts of plants‚ animals‚ and fungi. Today‚ the microscope is still a commonly used tool to diagnose illness in hospitals and clinics all over the world (1)
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T-φ gram Determination of Unreported Meteorological Quantities from T-φ gram 1. Mixing Ratio (W):- It is the ratio of the mass of water vapour to the mass of dry air containing the vapours at a certain temperature. It is expressed in gm/kg. On T-φ gram from dew point temperature we follow isohygric doted green line and read the value at the base. (It can be calculated at all levels where dew point temperature is available). 2. Saturation Mixing Ratio (Ws) :- Mixing ratio at
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INTRODUCTION The Gram Negative lab had two parts; the aim of the first part is to determine the concentration of gram negative bacteria in a water sample collected from a creek near Providence Road‚ Strickling. Gram negative bacteria have a cytoplasmic membrane‚ a thin peptidoglycan layer‚ and an additional outer membrane composed of phospholipids and lipopolysaccharides. Because gram negative bacteria have a relatively thin cell wall when compared to gram positive organisms‚ they are consequently
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unknown material was tube number twenty. I did a gram stain‚ oxidase test‚ and used three different medias to narrow down and identify my unknown organism. The first step I took in identifying was a gram stain. This gram stain would reveal whether my organism is gram positive or gram negative which would narrow my choices from fifteen options down to six. After completing the gram stain‚ my unknown was the color pink. This revealed my unknown as being gram negative. I also used the microscope to identify
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The aseptically inoculate nutrient broth tubes A and B did not have any microbes inoculated into the tubes so it should not have been cloudy and that was what was seen. Tube C should have been cloudy because it had E. coli inoculated into the nutrient broth and that was also seen. A simple streak was also performed and it looks has if the loop went over previous streaks which should not have been done. A complex streak was also done. No colonies grew towards the end of the streak‚ this could be due
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