| Gram Negative Unknown | Biology 3444-006 | | Lena Wallace | 11/7/2011 | | Abstract: The purpose of this lab was to identify an unknown bacteria culture using differential tests. The identification of the unknown culture was accomplished by identifying the bacteria based on its specific metabolic characteristics and morphology. It is suggested that culture 11 is a sample of Enterobacter aerogenes. Introduction: This experiment was centered on metabolic and biochemical testing
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I was able to come to the conclusion that my unknown is Klebsiella pneumoniae through the use of Bergey’s Manual. After performing a gram stain of my unknown I was able to determine that my unknown was a gram-negative rod. While testing the oxygen requirements my test tube had growth throughout the tube‚ indicating my unknown was a facultative anaerobe‚ which can grow in the presence or absence of oxygen. The most important test was whether my unknown was motile or nonmotile. My test tube only showed
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The plate count agar‚ CFC agar and cetrimide agar are used and provided different evidences on the isolation of Pseudomonas from the soil experiment. Firstly‚ the plate count agar is a medium for the enumeration of viable organisms in food‚ water‚ waste water and also from clinical samples‚ and thus it is non-selective to any species. Whereas the CFC agar is a selective agar which contains reduced amounts of cetrimide but also cephaloridine and fucidin are added to allow the isolation of most Pseudomonas
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Agar What is it and where is it from? Derived from the cell walls of red algae‚ agar is a gelatinous substance extracted through a process of boiling and filtration. Agar is specifically used in laboratories for diagnostics and experimental purposes. Agars main purpose is to act as a growth medium for micro-organisms such as bacteria and fungi. The micro-organisms feed off the nutrients contained in Agar and can be both cultured and observed within it by scientists and the use of a microscope. Using
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D4 H and E staining procedures Haematoxylin and eosin staining is a technique used by pathologists to add definition to nuclei and other parts of a cell in tissue samples under a microscope so that they can see it better when analysing the cells form and abnormalities. The H&E technique is the most commonly used in histology and the diagnoses of malignancies is based largely on the results of this procedure. The dyes will specifically stain elements of a cell or tissue because the dyes have a high
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(TLC) and consistent with its IR & 1HNMR spectra. The synthesized analogue was screened for antibacterial activity against one gram positive & two gram negative species. The compound exhibited good antibacterial effect towards gram negative species when compared to the standard ciprofloxacin. At the same time the analogue was retaining antibacterial activity towards gram positive species when compared to standard ciprofloxacin. The molecular docking studies showed a good correlation between their
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Gongora 12th December‚ 2014 MCB 3020L- Section 018 Unknown #16 Abstract This report contains the background information on gram positive and gram negative bacteria‚ which will aid in understanding the use of specific laboratory experiments to distinguish between the two types of bacteria. Included are the materials and methods used to identify the gram positive and gram negative bacteria and methods which also differentiate between microbes of each group. The implications of the methods of identification
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BIOSC 0050 Oct. 9‚ 2012 Dead or Alive? (Week 2) Post-Lab Assignment Results: The results of the Feulgen’s Schiff’s Stain test are that samples ALV-188-23 and ALV-843-20 turned magenta and samples ALV-882-21 and ALV-694-02 did not turn magenta. The result of the tetrazolium test is that all four of the samples did not turn pink. We were not able to conduct the detailed analysis of cultured plates with growth‚ so not results are available for this test. Discussion: For the Feulgen’s/Schiff’s
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Introduction By examining blood smear by microscopy it can detect infections whether they are viral or bacterial. Blood smears are examined by looking at the white and red blood cells appearances. The aim of the experiment is to compare a normal blood smear and its pathological features to those with an infection or abnormality by the use of microscopy looking at the cells involved with blood. A normal blood smear would contain 3 to 4 white blood cells per field in microscopy. If there is an
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In biology 102 lab‚ I conducted an experiment dealing with worms and how they regenerate or “grow”. We did this study over a specific type of worm called Planaria‚ which are simple‚ small‚ flat worms that reside in aquatic environments and are free-living (Brewton‚ 2017‚ p. 44). To start‚ I went over to the lab counters and obtained my worm with a plastic pipette‚ sucking up the worm with a little bit of water and placing it in a tiny covered plate. The water was essential to include with the worm
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