Antimicrobial Activity of Cilantro on Staphylococcus Epidermis and Escherichia coli Abstract Antimicrobial effects of cilantro against the gram positive bacteria S. epidermis and the gram negative bacteria E. coli were looked at. We predicted that cilantro would act as an antibiotic against S. epidermis but not to E. coli because S. epidermis does not contain an outer membrane whereas E coli. does. We predicted that the semi-permeable outer membrane of E. coli would protect the bacteria from
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the cell wall synthesis promoting osmotic instability‚ usually bactericidal. It hinders or kills bacteria. Ceftriaxone sodium is a broad-spectrum bactericidal cephalosporin antibiotic. Ceftriaxone is active in vitro against a wide range of Gram-positive and Gram-negative organisms‚ which include β-lactamase producing strains. Ceftriaxone is indicated in the treatment of the following infections either before the infecting organism has been identified or when known to be caused by bacteria of established
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‚ (2016) in vitro antimicrobial activity of Sudanese Cucumis melo L. fixed oil was investigated. Agar cup diffusion assay was performed against two fungal species (Candida albicans and Aspergillus niger)‚ two gram positive bacteria (Staphylococcus aureus and Bacillus subtilis) and two gram negative bacteria (Escherichia coli and Pseudomonas aeruginosa). Ampicillin‚ gentamycin and clotrimazole were used as positive controls. Antimicrobial activity was shown by all the species except E.coli and P.
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Escherichia coli and Staphylococcus aureus. The effectiveness of the chemical agent was determined by the size of the zone of inhibition around the disc incubated on soft nutrient agar seeded with the two bacteria on separate plates. For E. coli‚ a gram negative organism‚ the zone of inhibition was the largest with Lysol (0.2mm). Next‚ Scope has slightly smaller zone of inhibition‚ 0.15mm‚ on the organism. Hydrogen peroxide‚ Wescodyne and 70 percent Ethyl Alcohol had no affect on the bacteria. Inhibition
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DIFFERENT TYPES OF AGAR Mac Conkey‘s Agar plate MacConkey (also McConkey) agar is a culture medium designed to grow Gram-negative bacteria and stain them for lactose fermentation. It contains bile salts (to inhibit most Grampositive bacteria‚ except Enterococcus and some species of Staphylococcus)‚ crystal violet dye (which also inhibits certain Gram-positive bacteria)‚ neutral red dye (which stains microbes fermenting lactose)‚ lactose and peptone. QUALITY CONTROL Results after 24 hrs at
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IMPORTANT BACTERIA | GRAM-POSITIVE BACTERIA * Alpha and gamma hemolysis on blood agar * Bacillus cereus colonies on blood agar * Beta-hemolysis on blood agar (S.aureus) * Beta-hemolysis on blood agar * Beta-hemolytic colonies on blood agar * Clostridium perfringens Gram stain * Corynebacterium diphtheriae Gram stain * Corynebacterium pseudotuberculosis on blood agar * Enterococcus faecalis * Enterococcus faecalis on blood agar * Enterococcus faecalis Gram stain * Enterococcus
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In general‚ members of the genus Vibrio are Gram-negative‚ motile and most of the members have a single polar flagellum‚ when grown in liquid medium (Kaysner & DePaola‚ 2004). Vibrio cholerae lives naturally in riverine and is the causative agent of cholera outbreaks and epidemics. Vibrio cholerae is comma-shaped. Initial isolates are slightly curved‚ whereas they can appear as straight rods upon laboratory culturing. Vibrio cholerae is a facultative anaerobe and can undergo respiratory and fermentative
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Identifying Unknown Bacteria Using Biochemical and Molecular Methods Beginning of Instructor Pages Instructor Pages - - 3 Purpose The purpose of this lab is to introduce a variety of lab techniques to students working on the common problem of identifying an unknown bacterium. This lab helps students develop an understanding of the biochemical and molecular differences in bacteria and introduces the concept of identifying species based on characeristic gene sequences. Students work through
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Question How many calories per gram are contained in marshmallows? Introduction Food is the primary source of energy for all living organisms - it is used to replenish and supply energy to the body and provide the nutrients it needs to grow and thrive. In 1824‚ Nicolas Clement came up with the term “calorie” as a unit of heat energy (Calorie n.d.). In modern day‚ calories are often associated with food because the number of calories in food is the measure of how much potential energy that food
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Kcalories per gram but I thought that popcorn would have the highest Kcalories per gram. It was hypothesized that popcorn would have the highest amount of Kcalories per gram when burned. My claim is true in the theoretical conditions. According to the data collected in the experiment‚ it shows that rice cakes had the highest experimental Kcalories per gram at 1.141 Kcalories per gram. I hypothesized that popcorn would have the highest Kcalorie per gram but it had the 2nd most Kcalorie per gram at 1.11
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