(bacilli)‚ and spirals Diversity of prokaryotes Gram negative vs. gram positive bacteria (Fig. 27.3) Gram + vs - -: less peptidoglycan -: outer membrane -: lipopolysaccharides in outer membrane Why are gram– more pathogenic? Lipopolysaccharides are often toxic Outer membrane protects against the body’s defences Outer membrane impedes entry of antibiotics Common antibiotics inhibit cross-linking of peptidoglycan‚ particularly in gram+ Motility ? Most motile bacteria propel themselves
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impression that the litter level are the determinant of the cleanliness of the water. This study intends to examine: The bacteria population of samples obtained from several site across south east coast of England. Identify species of Gram Negative (-) & (+) of each sample. identify any faecal coliform bacteria which might suggest water contamination. These indicator organisms are used for indication of water contaminant by human and other warm-blooded animals (APHA 1992). Several
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Generic name | Brand names | Common uses[2] | Possible side effects[2] | Mechanism of action | Aminoglycosides | Amikacin | Amikin | Infections caused by Gram-negative bacteria‚ such asEscherichia coli and Klebsiellaparticularly Pseudomonas aeruginosa. Effective against Aerobic bacteria (not obligate/facultative anaerobes) andtularemia. | * Hearing loss * Vertigo * Kidney damage | Binding to the bacterial 30S ribosomal subunit (some work by binding to the 50S subunit)‚ inhibiting the translocation
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Rocky Mountain Spotted Fever Rickettsia rickettsia was named after the first person who discover Rocky Mountain Spotted fever‚ Howard Taylor Ricketts. The unicellular aerobic gram negative non-motile‚ non-spore forming‚ coccobacilli bacterium are 0.3-0.5 x 0.8-2.0 um. It is in the family Rickettsiaceae and order Rickettsiales of the α-Proteobacteria. It is a unicellular microorganism that lacks a distinct nucleus and membrane- bound organelles‚ while it is a three-layered cell wall and its outer
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Prokaryotic cell walls are constructed of many layers‚ which is formed from peptidoglycan polymers cross-linked by peptide side chains. 9.) Describe a Gram Stain. Staining technique that divides bacteria into gram-negative or gram-positive based on retention of a violet dye 10.) Distinguish between gram positive and gram negative bacteria. Gram-positive- has a
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Microbiology Final Exam Question 1 2 out of 2 points Full induction of the lactose operon requires ________. Question 2 0 out of 2 points The enzymes that help pack DNA into the cell by coiling the DNA into a tight bundle are ________. Question 3 2 out of 2 points Gene regulation can involve a protein repressor that blocks ________ from initiating transcription. Question 4 2 out of 2 points Synthesis of a repressible enzyme is inhibited by ________.
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BSC 2010C.LAB TH 7-9:50pm 29 August 2013 Biology Lab Report Lab #1 –PROTEIN EXTRACTION LAB I. INTRODUCTION To begin the process of protein extraction and compare the results in a study‚ it is necessary to understand the importance of proteins‚ the process of extraction and how you are using the results to determine a rational conclusion. First understand proteins and the necessity of studying their impact. Proteins are essential molecules for biological functions and are the
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Multiplication of contaminant bacteria in urine and interpretation of delayed culture. Abstract A prospective study of the bacterial populations of non-infected urine was mounted in an attempt to define the length of delay between voiding and analysis during which culture would not give false positive results due to the multiplication of contaminant bacteria present at the time of voiding. The findings suggest that culture of urine within four hours of voiding is likely to give a true indication
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BIOL 3350 Spring 2013 Unit 1 Review 1. Define Anatomy The science of the structure of the body and its relationships. 2. Define Physiology Study of the physical and chemical processes involved in the functioning of the human body. 3. Define Metabolism. Describe the two metabolic pathways? Set of chemical reactions that occurs in living cells to maintain life. Catabolism is cellular respiration and anabolism is the construction of proteins. Complete set of chemical
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diff‚ is a spore-forming‚ gram-positive‚ anaerobic bacillus (The Mayo Clinic). Although the active bacteria are gram-positive‚ the spores are gram-negative (Horridge‚ et al). Culture of c-diff is performed at normal human body temp‚ on a blood agar (Nadan). After 48 hours of incubation‚ isolates resembling C. diff are anaerobically subcultured on a fastidious anaerobe agar. After the final culture‚ colonies are checked for florescence‚ and gram stained. Once gram-staining is complete‚ colonies are
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