Abstract This experiment was carried out to perform staining methods on microbes‚ explain the mechanisms of staining‚ namely‚ simple staining‚ and learn how to use the microscope. The experiment setting was kept as sterile as possible when conducting the experiment. The microbes under the microscope were drawn out and labelled. In conclusion‚ different staining techniques are used to determine the presence of certain exterior structures of the bacteria like the cell envelope or just to identify
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Differentiating Organisms using the Gram Stain Introduction The experiment conducted was based upon the known attributes of two different groups of bacteria‚ those that are gram positive‚ and those that are gram negative. Using a specific staining procedure‚ it is possible to differentiate the two types under a microscope The gram stain method of differentiation is possible because of differences in the cell membrane between the two categories of bacteria. Gram positive cells have an extra thick
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Microscopy and Staining Abstract The primary focus of this lab was on microscopy and simple stains. Microscopy that was used were magnification‚ slide preparation‚ and staining. Methylene blue‚ a simple staining component‚ was used to stain the slide in order to see the different microbes and determine their cellular shape. Introduction The purpose of this lab was to become familiar with the light microscope and how to accurately use it to view specimens. The staining method was used
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1. Describe the major differences between gram positive and gram negative bacteria cell walls. The gram negative bacteria cell wall is a thin peptidoglycan layer and an outer cell membrane with a lipopolysaccharide layer. The gram positive bacteria cell wall is a single thick peptidoglycan layer. This wall forms in a mesh like formation of three layers of alternating material. 2. From the procedure that you have carried out‚do you feel that the Gram positive stain is a simple procedure? No‚
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techniques. Here they are; Simple staining: Only one type of dye used for simple staining and it stains all cells the same color. Creating contrast between cell and background to observe cell morphology is aimed. Differential staining: A differential stain uses more than one dye and stains different kinds of organism with different colors. Positive staining: If cell itself is stained it is called positive staining. It sticks with specimen and gives its color. Negative staining: If background is stained
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culture‚ I immediately made a streak plate and started the Gram Stain protocol1. In the first two slides‚ I made and stained‚ my results were ambiguous. Without putting the slide into the microscope‚ I noticed one was pink and the other purple. To resolve it‚ I discarded my slides and started again and was extremely careful in proceeding the Gram Stain. I concluded that my unknown A was gram-negative bacilli. Using the dichotomous key for gram-negative bacilli I started the protocol for the lactose
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Bio Lab Report Title: Gram-Negative and Gram-Positive contamination in yogurt. Introduction: There is bacteria that is beneficial and harmful in the human. Yogurt was choosed to be the best example that contains this bacteria. What happens to be the difference between these two types of bacteria‚ and how are they harmful? The bacteria is considered “Gram-Negative‚” and the substance is considered contaminated when there is more negative bacteria than positive bacteria. This will prove or disapprove
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February 2011 Vol 55 Chief Editor : Neeta Prasad Joint Director (Prod) : J.K. Chandra Editor : Manogyan R. Pal Cover Design : Manjula Das E-mail (Editorial) : editoryojana@hotmail.com : yojanace@gmail.com (Circulation) : pdjucir_ jcm@yahoo.co.in Website : www.yojana.gov.in Let noble thoughts come to us from every side Rig Veda CONTENTS Grassroots Level Democracy in India : An Assessment...........................................................................5 Mathew
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(2005) defined staining as the method of artificially producing color in microorganisms to allow for the visualization under the microscope. He further stated that stains are used not only to make the organisms visible but also to understand their structure and chemical nature. Gram staining is a technique commonly used in the laboratory. It is used to differentiate two large groups of bacteria based on their different cell wall constituents. It distinguishes between Gram positive and Gram negative groups
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A Comparative Study of Dalandan and Kamias as Decolorizer in Gram’s Staining Staphylococcus aureus and Escherichia coli An Undergraduate Research Presented to The College of Medical Technology Centro Escolar University In Partial Fulfillment Of the Requirements of the Degree Bachelor of Science in Medical Technology By: Aduana‚ Frances Mae D. Barnachea‚ Erika Joy V. Decleto‚ Pearl Yvette P. Lagmay‚ Ira Alexis C. Paris‚ Dublin Justin Patron‚ Chronielle Albert C. Radovan‚ Denzel James C
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