performed within a certain time frame the specimen will become contaminated. Part II: Bacterial Anatomy The purpose of this experiment is to become familiar and gain an understanding of how morphology and the arrangement of bacteria relate to staining techniques. Sample 1 In this study a female patient who had a problem that had arisen suddenly after lunch‚ had allergic reaction to something that she ate or drank had sent her to the bathroom with cramps and copious diarrhea. She knew she
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bacteria? Gram Stain This separation is based on differences in what physical trait? The structure of the cell walls. What is the iodine used for in this staining technique? Crystal violet is first used to stain the sample and iodine is used to affix it. Why is it important to heat fix the bacteria prior to the staining procedure? This procedure is done to kill any bacteria that may be on the slide already and this will insure a clean slide. If you had a bacteria sample that you knew to be gram positive
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Bacteria Morphology April 2‚ 2015 Abstract – The Purpose of this exercise is to gain experience in bacterial morphologies in prepared wet-mounted slides and interpreting the findings of bacteria through direct and indirect staining technique. Hypothesis – The experiment will allow for further insight into stained organisms‚ allowing extended contrast‚ differentiating shape and structure through utilization of microscope. Procedure – Instructions followed as per procedure‚ specimens
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NAME: PRAJWAL BHARADWAJ Ampicillin‚ Vancomycin‚ and Tetracycline Effectiveness on Gram Positive and Gram Negative Bacteria. INTRODUCTION: Bacteria are microbial organisms which are present in various environments. Many bacteria are good and help humans synthesize materials and assist in biological processes‚ such as digestion. However‚ some bacteria can cause harmful diseases. When harmful bacteria infects the body‚ people take antibiotics in order to suppress bacteria. Different antibiotics have
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two slides for gram staining and viewing under a microscope. {Either my gram-stain slides were bad or the microscopes I chose for viewing were not good. No bacteria were found under the microscope.} Prepare another gram stain of the unknown bacteria. {Gram-positive‚ coccus shaped bacteria were observed under the microscope. Also noted‚ the slide may have been inadvertently contaminated during the gram stain process by another person in the laboratory.} [I do think that the gram-stain result
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The tests used to confirm the fermentation were a gas production test‚ pH test‚ Gram stain‚ and turbidity test. The results showed during the conversion of milk to Kefir there was gas produced‚ a decrease in pH‚ more bacteria present‚ and an increase in absorbency. The results proved that fermentation occurred with three positive confirmatory results from the gas production‚ pH‚ and turbidity tests‚ while the Gram stain test acted as a negative confirmatory test. Introduction: The main goal of
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physiological class of bacteria capable of utilising agar as a sole carbon source. This ability is made available by the use of agarases - enzymes which break down agarose into oligosaccharides. This physiological class branches through genii‚ regardless of Gram Stain status or morphology. Through a review of scientific literature we can find identification methods‚ optimum conditions and the general function and location of agarolytic bacteria‚ as well as methods to culture them in vitro for study and experimentation
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included: Gram stain‚ mannitol salt agar‚ coagulase tube test‚ and an antimicrobial susceptibility test. The tests selected were based on the results of a gram stain. Gram staining‚ the most commonly used differential stain‚ allows for the fast and easy detection between gram negative and gram positive organisms based on the presence or absence of peptidoglycan. A gram positive organism resists the decolorization process due to a large amount of peptidoglycan and appears a purple color. A gram negative
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the knowledge gained from the sessions in the laboratory‚ we can now integrate what we have learned to the process of finding out the unknowns given. Materials and Methods The professor gave out the unknown specimens. It contained one-‐gram positive and one-‐gram negative bacteria from the given list. I was assigned unknown A. The process of identification was achieved by utilizing procedures learnt during the semester. Procedures were followed as stated in the lab manual (1). Since the sample contained
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Exercise 5 Introduction: Many different species of bacteria look similar under the microscope and also have the same staining results (ex. Gram stain). To be able to differentiate between the different species‚ one can look at the metabolic differences (fermentation)‚ as well as the environmental condition differences (temperature‚ pH‚ oxygen requirements). Being able to manipulate these conditions in a controlled environment can help to correctly identify the exact bacteria. Different media
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