Microscopy and Staining Abstract The primary focus of this lab was on microscopy and simple stains. Microscopy that was used were magnification‚ slide preparation‚ and staining. Methylene blue‚ a simple staining component‚ was used to stain the slide in order to see the different microbes and determine their cellular shape. Introduction The purpose of this lab was to become familiar with the light microscope and how to accurately use it to view specimens. The staining method was used
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Abstract This experiment was carried out to perform staining methods on microbes‚ explain the mechanisms of staining‚ namely‚ simple staining‚ and learn how to use the microscope. The experiment setting was kept as sterile as possible when conducting the experiment. The microbes under the microscope were drawn out and labelled. In conclusion‚ different staining techniques are used to determine the presence of certain exterior structures of the bacteria like the cell envelope or just to identify
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used to obtain a pure culture of a Gram positive coccus genus of bacteria. Several biochemical tests will be performed to aid in the identification of this unknown bacterium. Biochemical tests are a series of tests used to identify certain bacterium The various tests that are used in this lab are the catalase test‚ oxidase test‚ blood hemolytic test‚ MSA‚ blood agar‚ and PEA/ab sens test. A known Gram positive coccus bacteria will be used alongside an isolated Gram positive unknown bacteria for comparison
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Lab 5 –Cell Structure and Staining using Microscopy Instructions: Please download this MSWord document to your computer and answer the questions as asked. Then save the document and upload it to Bb using the Assignment feature provided. This assignment is worth a total of 100 points – there are 20 questions worth 5 points each. NAME Buket Rembert In Lab 3 you were introduced to microscopy. In this lab you will be adding to that experience by reviewing the differences in cell structure for Prokaryotes
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techniques. Here they are; Simple staining: Only one type of dye used for simple staining and it stains all cells the same color. Creating contrast between cell and background to observe cell morphology is aimed. Differential staining: A differential stain uses more than one dye and stains different kinds of organism with different colors. Positive staining: If cell itself is stained it is called positive staining. It sticks with specimen and gives its color. Negative staining: If background is stained
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belts‚ caps‚ etc‚ are the most preferred clothing for today’s youth. This study discusses compositions and methods of reducing or preventing back staining of indigo dyes on the weft yarn and pocket of denim garments. The dye‚ as it is removed from the denim material‚ post treatment with cellulase or by a conventional washing process may cause ‘back staining’ or ‘redeposition’ on the denim material; e.g : re-coloration of blue threads and blue coloration of white threads‚ resulting in less contrast between
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Gram Stain: Gram is an empirical method to distinguish the species of bacteria into two groups Gram-positive and Gram-negative based on the physicochemical properties of the cells. First‚ a smear was prepared by use a sterile transfer loop that been flamed to removes some bacteria from slant agar and placed on the slide; mixed with one drop of water and let air dried. After dried‚ heat fixation the slide by passed the slide over a flame quickly 2-3 times to stick bacteria to the slide. Next‚ the
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Momordica charantia L. Extract as a Prophylactic Medicine for Gram-Negative Bacteria Escherichia coli Proponents: John Rovic P. Ngo Lester T. Almarez Trisha Mae N. Reyes August 2013 In Partial Fullfillment of the Requirements in Research II-B Momordica charantia L. Extract as a Prophylactic Medicine for Gram-Negative Bacteria Escherichia coli A Science Research Project Presented to The Faculty of Science and Technology Mr.June Hayden Sinson Research Adviser
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Chemistry 1 February 3‚ 2014 Harvesting CaCo2 in a precipitate reaction results in less grams desired amount (2 grams) Abstract: The objective of this experiment was to synthesize 2.00 grams of CaCo2 for a hypothetical manufacturing company using the two least detrimental (in regards to health and the environment) and least expensive chemicals possible through a precipitate reaction. A precipitate reaction happens when cations and anions of certain aqueous solutions react and combine forming
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The Effect of Common Bactericidal Agents on Staphylococcus Epidermidis Introduction Staphylococcus epidermidis is a facultative anaerobic bacterium. It is part of the normal human flora and is found on the skin. Colonies of these bacteria can produce a protective slime called a hydrophobic biofilm. Staphylococcus epidermidis is usually not pathogenic unless it enters the human body. One of the most common places for infections are hospitals where people often have weakened immune systems‚ open
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