"Gram staining staphylococcus aureus and pseudomonas fluorescens" Essays and Research Papers

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    Bibliography: © The McGraw−Hill Companies‚ 2002 E X E RC I S E © The McGraw−Hill Companies‚ 2002 Gram Positive 38. General Unknown © The McGraw−Hill Companies‚ 2002

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    Tisis

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    A Comparative Study of Dalandan and Kamias as Decolorizer in Gram’s Staining Staphylococcus aureus and Escherichia coli An Undergraduate Research Presented to The College of Medical Technology Centro Escolar University In Partial Fulfillment Of the Requirements of the Degree Bachelor of Science in Medical Technology By: Aduana‚ Frances Mae D. Barnachea‚ Erika Joy V. Decleto‚ Pearl Yvette P. Lagmay‚ Ira Alexis C. Paris‚ Dublin Justin Patron‚ Chronielle Albert C. Radovan‚ Denzel James C

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    investigations of each unknown in Table 1 by completing the following steps: 1) Apply the stain to your first unknown slide and examine it under the microscope. 2) Record the shape of the bacteria‚ the arrangement of the bacteria‚ and the gram staining characteristics. 3) Analyze and record the G+C content of the sample by dragging the DNA tube that corresponds to the bacterial sample to the GC Content Measuring Apparatus. (Note: the identification of the DNA tubes may be confusing;

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    samples determined using agar plate method. Differentiations and characterizations of various isolates were based on biochemical reactions and gram-staining technique. Frozen mackerel fish (Scomber scombrus) was used for the study. Invitro assay result revealed that the samples were predominantly contaminated by three bacteria species viz: Staphylococcus aureus‚ Escherichia coli and Lactobacillus plantarum. Incidences of the various isolates in the culture were found to be 60‚ 20 and 15%‚ respectively

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    I also sterilized the top of the test tube just for contamination purposes. Next I dipped my inoculation loop into the test tube to gather a sample of Staphylococcus aureus. Once I had my sample‚ I then began using the strike method to inoculate my TSA plate. I made sure to flame sterilize my loop between each strikes. I continued on using this same process to inoculate the three remaining TSA plates with the

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    distinguishing between Gram-positive cocci in chains (catalase negative) versus Gram-positive cocci in clusters (catalase positive). The coagulase test is used to differentiate Staphylococcus aureus from coagulase-negative staphylococci. The test uses rabbit plasma that has been inoculated with a staphylococcal colony. The tube is then incubated at 37 degrees Celsius for 1½ hours. If negative then continue incubation up to 18 hours. * If positive (i.e.‚ the suspect colony is S. aureus)‚ the serum

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    November 19‚ 2008) ABSTRACT The aim of this study was to investigate the activity of (28%) ethanolic extract propolis (EEP) of [Bazian‚ Pshdar‚ Sharbazher‚ Khormal‚ Kanakawa and Sulaimani (city center)] against (Staphylococcus aureusStaphylococcus epidermidis‚ Escherichia coli‚ Pseudomonas aerugenosa‚ Klebsiella pneumoniae‚ Proteus mirabilis and yeast Candida albicans). Two methods were employed for propolis activity evaluation; firstly by agar well diffusion method using 40µl of propolis extracts

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    Trypticase Soy Agar plate to isolate individual colonies to be studied‚ tested and identified. After incubation of the individual TSA plates‚ the morphologies were viewed and noted and a Gram stain was completed on each individual bacterium‚ which will be referred to as Bacteria #1 and Bacteria #2. After the Gram reaction was determined on Bacteria #1 and Bacteria #2‚ different biochemical tests were done according to the dichotomous keys provided in the lab manual. All the tests were performed by

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    microbiology lab

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    Abstract The primary focus of this lab was on microscopy and simple stains. Crystal violet and Carbol fuchsine‚ simple staining components‚ were used to stain the slide in order to see the different microbes in order to determine their cellular shape and identify unknown ones by comparing. Introduction Bacterial cells are usually colorless because cytoplasm‚ for the most part‚ is transparent. Since the bacteria are colorless‚ it is almost essential to add a stain to make the bacteria more visible

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    Unknown report Micro

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    differential tests indicated that unknown culture 5 is Staphylococcus aureus. Introduction: The purpose of this lab was to determine the identity of an unknown bacteria slant culture using a series of differential tests. The tests used to identify the unknown bacterial culture included: Gram stain‚ mannitol salt agar‚ coagulase tube test‚ and an antimicrobial susceptibility test. The tests selected were based on the results of a gram stain. Gram staining‚ the most commonly used differential stain‚ allows

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