GRAM STAIN THEORY: The most widely used staining procedure in microbiology is the Gram stain‚ discovered by the Danish scientist and physician Hans Christian Joachim Gram in 1884. Gram staining is a differential staining technique that differentiates bacteria into two groups: gram-positives and gram-negatives. The procedure is based on the ability of microorganisms to retain color of the stains used during the gram
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Gram Staining of Bacteria Since bacteria is really small microbiologist use stains to help them see bacteria more clearly under the microscope. Many of the stains they use color the bacteria cells directly and are called direct stains. Bacteria cells have a slightly negative charge while direct stains have a slightly positive charge that helps the stain bin to the bacteria. The strength of the binding from the stains depends on the make-up of the cell wall itself. Gram staining is the most helpful
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proficiency and should be able to: A. describe staining; B. enumerate the common stains; C. differentiate between an acidic dye and a basic dye; D. compare simple‚ differential and special stains; and E. list the steps in preparing a Gram stain and describe the appearance of a gram-positive and gram- negative cells after each step.\ II. SUBJECT MATTER: Biological Techniques Topic: Staining A. Materials: * cartolina- used during
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Introduction:The objective throughout the gram staining experiment was to classify bacteria through their chemical nature as well as the physical structure of their cell walls. Through this experiment one would be able to observe either a thick or thin layer of peptidoglycan by the appearance of the bacteria staining either gram-positive blue-purple or gram-negative pink. Question: Is there a subsequent effect of the bacteria when decolorizing with alcohol? Hypothesis: If there is either a removal
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MRSA: MRSA is an acronym used to describe a strain of Staphylococcus aureus that is resistant to the killing effect of the antibiotic methicillin. Methicillin-resistant Staphylococcus aureus is often called a ‘superbug’ because it is very difficult to treat. It causes a huge number of infections every year in hospitals all over the world. MRSA occurs most often in the U.S. In Europe‚ the problem doesn’t seem quite as bad as in the U.S partly because of differences in the prescribing of antibiotics
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What is Staph? Staphylococcus aureus is a bacteria that is commonly found on a person’s skin and inside their sensitive mucous lined areas‚ such as the inside of the nose. Usually‚ it is harmless because of the natural protective barriers that the body has. But‚ if the skin has any damage done to it‚ such as a scratch or scrape‚ this bacteria can quickly invade the wound‚ which in turn causes a staph infection. Treating Staphylococcus Infections If the infection is on the surface of the skin‚
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Methicillin-resistant Staphylococcus aureus (MRSA) Jessica Heinen Autry Technology Center Introduction The full name of MRSA is meticillin-resistant staphylococcus aureus. Staphylococcus aureus (also known as staph) is a common type of bacteria. It is often carried on the skin and inside the nostrils and throat‚ and can cause mild infections of the skin such as boils and impetigo. If staph bacteria get into a break in the skin‚ they can cause life-threatening infections‚ such as
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Staphylococcus aureus: From a Spot to the Hospital The hospital is silent this morning‚ as the sun breaks through the blinds and casts off all the silver objects in the room. My hospital bed is uncomfortable‚ and it makes me yearn for my 100-year-old mattress and many unnecessary plush-pillows. The smell of bleach and cleanliness lingers in my nose and almost makes me feel sicker. How did I end up here? Well‚ that is not an adrenaline rushed injury or a freak accident‚ but something that started
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The problems that arise regarding the antibiotic resistance of certain bacteria has continue to worry the health industries. One of these bacteria is the Staphylococcus aureus. It has been one of the major causes of hospital-acquired infections which has resulted to a lot of deaths each year. Description Staphylococcus aureus is a gram-positive bacteria which indicates that it has a cell wall structure with a thick layer of peptidoglycan. It is a facultative anaerobe which allows it to perform
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Gram Staining Introduction Prokaryotes are a large group of organisms with no membrane bound organelles. They consist of two domains: Archaea and Bacteria. These organisms are only found in extreme environments such as volcanoes. Prokaryotes are still being researched and are a very diverse group. In this lab we focused on trying to identify if the bacteria found had a lot of peptidoglycan by gram staining. Testing this could be done by using a Petri dish full of agar and testing different bacteria
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