Techniques and Gram Staining Method Results Table 1. Gram stain reaction and cellular features of the culture. Gram staining methods were applied on the given mixture of Bacillus cereus‚ Escherichia coli and Staphylococcus aureus and then examined microscopically. Results were recorded in Table 1. Gram Reactions Cell shapes Cell Ends and Arrangement Size Distinctive Characters Predicted Bacteria Bacteria 1 Gram positive (purple) Cocci Rounded‚ clusters‚ singly Small - Staphylococcus aureus Bacteria
Premium Gram staining Bacteria Escherichia coli
Stainin g What is Cellular Staining? Cell staining is a technique that can be used to better visualize cells and cell components under a microscope. By using different stains‚ one can preferentially stain certain cell components‚ such as a nucleus or a cell wall‚ or the entire cell. Most stains can be used on fixed‚ or non-living cells‚ while only some can be used on living cells; some stains can be used on either living is or non-living What Cellularcells. Staining? The most basic reason that cells
Premium Staining Bacteria Microscope
Tittle Gram stain Experiment Date 24October2014 Submission Date 31October2014 Students Name Tittle Gram stain. Objectives Aim of this experiment is to differentiate between the two major categories of bacteria ‚ gram positive and gram negative. Through this experiment‚ gram staining skills develop. More understanding the types and morphology of bacteria. Expected experimental result‚ Escherichia coli (E.coli ) is a negative gram bacteria which stain pink colour ‚ while Staphylococcus aureus
Free Bacteria Staining Gram staining
Pseudomonas aeruginosa Pseudomonas aeruginosa is the bacteria of my initial unknown project. Determining which bacteria I had was completed by many steps. The one fact that I had‚ was that it was a gram negative bacteria because on the gram stain it appeared pink. Its cell wall is composed of a plasma membrane‚ periplasmic space‚ peptidoglycan and an outer membrane (lipopolysaccharide and protein). By looking at the agar plate it was easy to tell that it was not a swarmer and it did not have any
Premium Bacteria Microbiology Gram staining
Staphylococcus epidermidis is one of the most prevalent microorganisms found on the human skin and in the mucous membranes‚ however‚ it is a typically overlooked bacterium because there is very little that is known about it. Though it is not as aggressive as its cousin Staphylococcus aureus‚ Staphylococcus epidermidis is the most frequent cause for nosocomial pathogens‚ especially among newborns‚ the elderly‚ and anyone who has a compromised immune system. These infections are usually associated
Premium Bacteria Immune system Staphylococcus
Differential Staining Cynthia Alonzo‚ M.S. Version 42-0242-00-01 Lab RepoRt assistant This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions‚ diagrams if needed‚ and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information
Premium Gram staining Staining Bacteria
Investigating the Growth of P. fluorescens Biofilms at the Air-Liquid Interface and the Variations in Biofilm Integrity Abstract Pseudomonas fluorescens produces biofilms in a variety of environments‚ and in experimental situations has been shown to develop random mutations resulting in a phonotypical change in the resulting biofilm. One particular mutation resulted in the growth of a biofilm known as the Wrinkly Spreader‚ which produced a thicker‚ stronger biofilm that had a stronger attachment
Premium Bacteria Pseudomonas aeruginosa Archaea
Staphylococcus Aureus (Staph Aureus) is a gram-positive coccal bacterium and is a member of the Firmicutes phylum. It is most commonly found in the respiratory tract or in skin infections and can sometimes occur from food poisoning. This bacterium is very common and can be found in almost all parts of the world. Every year around 500‚000 people in the USA go to the hospital to get treated (National Institutes of Health‚ 1999). Staph Aureus has genetically developed to resist many antibiotics (Larsen
Premium Bacteria Staphylococcus aureus Tea tree oil
1. Describe the major differences between gram positive and gram negative bacteria cell walls. The gram negative bacteria cell wall is a thin peptidoglycan layer and an outer cell membrane with a lipopolysaccharide layer. The gram positive bacteria cell wall is a single thick peptidoglycan layer. This wall forms in a mesh like formation of three layers of alternating material. 2. From the procedure that you have carried out‚do you feel that the Gram positive stain is a simple procedure? No‚
Free Bacteria Staining Microbiology
MBK – Lab Report Differential Staining Questions: A. What is a differential stain? How is it different from a simple stain? 1. A differential stain is a stain that differentiates between gram-positive and gram-negative bacteria. Bacteria that stain purple are considered gram-positive and those that stain pink are considered gram-negative. 2. A simple stain is a stain that reacts with all microbes in and identical fashion. They are used to increase contrast. 3. They
Free Bacteria Staining Gram staining