F-lac-strr) underwent conjugation to produce a transconjugant strain (F’lac+strr). MAC plates and streptomycin were utilized to determine if conjugation had occurred. When plated‚ the donor colonies appeared red and the recipient colonies appeared white. The transconjugant plates showed red and white colonies. Using alkaline lysis miniprep‚ a DNA plasmid was isolated from the donor and transconjugant strains and FIGE electrophoresis was used to determine the size of the plasmid. The conjugation efficiency was
Premium Plasmid Bacteria Molecular biology
Grammatical categories The grammatical categories which are realised by the described types of forms organised in functional paradigmatic oppositions‚ can either be innate for a given class of words‚ or only be expressed on the surface of it‚ serving as a sign of correlation with some other class. For instance‚ the category of number is organically connected with the functional nature of the noun; it directly exposes the number of the referent substance‚ e.g. one ship — several ships. The category
Premium Inflection
Grammatical Categories of Speech The main grammatical categories are Determiner‚ Auxiliary‚ Coordinator‚ and Complementizer. As also mentioned above‚ it is hard to define grammatical categories in terms of meaning because they have very little. Their function is to make the lexical categories fit together. • Determiner (D) The determiner category includes the articles a(n) and the‚ as well as demonstratives‚ possessive pronouns‚ possessive nouns‚ some quantifiers‚ some interrogatives‚ and
Premium Grammar Pronoun Sentence
SUBJECT ASSIGNMENT: APPROACHES TO LANGUAGE IN THE CLASSROOM CONTEXT GENERAL INFORMATION: The subject assignment consists of doing three short exercises. To do so‚ keep the activities statements and answer just below them. This assignment must be done in groups of two and has to fulfil the following conditions: Length: between 6 and 8 pages (without including cover‚ index or appendices –if there are any-). Type of font: Arial or Times New Roman. Size: 11. Line height: 1.5. Alignment: Justified
Premium Language acquisition Linguistics Language education
December 21‚ 2012 The Whipping 1. Who is the speaker? What kind of person is the speaker? a. The speaker could be a neighbor or passenger who observes a domestic scene that reminds him of one similar to his own.. The speaker has a strong sympathy about the boy but also is a coward because the speaker did not stop the woman. 2. Is there an identifiable audience for the speaker? What can we know about it? a. There is no identifiable audience. The speaker is just noticing the woman beating
Premium Grammatical person
8 l SUFFIXES l l l The Suffixes are letters added to the end of words to make new words. (The suffixes change the word class of the words they attached to) - Common Noun Suffixes: 1. –er / -or: these suffixes are added to Verbs to derive nouns of a person who does the a action. Verbs + –er ــــــــــــــــــــــــ> noun of a person
Free Noun Adjective Verb
CONTENTS Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 Part 1 The English adjective . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Part 2 Old English adjective . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 Part 3 Middle English adjective. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 Part 4 Early Modern English
Premium English language Inflection Middle English
subjunctive Answer Selected Answer: removamur Response Feedback: male‚ please check the conjugation of your verb and how to form the present subjunctive again. Question 2 0 out of 1 points committo‚ third plural present active subjunctive Answer Selected Answer: committent Response Feedback: male‚ please check the conjugation of your verb and how to form the present subjunctive again. Question 3 0 out of 1 points
Premium Grammatical person
COMPOUND PERSONAL PRONOUNS: NOTES: 1. Reflexive pronouns act as the object of the verb‚ but they reflect the action back to the subject. These pronouns will always be the same person or thing as the subject. Ex. She blamed herself for the mishap. He hurt himself yesterday. 2. Intensive pronouns simply add force or emphasis to a noun or pronoun that is already in the sentence. Ex. You yourself wrote those words. I prepared the meal myself. EXERCISES: Tell whether the underlined
Premium Grammatical tense
Proofreading Out Loud Proofreading is a method of editing a document. Proofreading aloud is technique that engages the reader fully into the process. I generally proofread either to myself or in a low voice and have always been able to make corrections in this fashion. However‚ I do find that when I read out loud‚ it helps me to discover mistakes I might otherwise overlook. Mistakes like grammar‚ punctuation‚ sentence flow or word usage. Overall‚ proofreading out loud allows you to not just catch
Premium Verb Word Orthography