research involved the occurrence of genetic transformation in bacteria (E. Coli). More specifically‚ a previously prepared pGLO plasmid--which consisted of the gene to be cloned--was used to transform non-pathogenic bacteria. The pGLO plasmid contained a gene for the Green Fluorescent Protein (GFP) from a bioluminescent jellyfish and a gene for resistance to ampicillin‚ an antibiotic. Essentially‚ we wanted to determine the conditions of the bacteria that would glow. Our hypothesis was that the transformed
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are capable of inhibiting or killing the bacteria. Antibiotics have enabled the effective treatment of infections including life threatening diseases ranging from respiratory diseases to sexually transmitted diseases (Rang et al.‚2007). An antibiotic acts by either limiting or stopping the growth of bacteria. It accomplishes this by probably interfering with the cell wall of the bacteria while having minimal effect on the normal body cells. Classifying bacteria into classes helps in identifying the
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What are bacteria? Bacteria are tiny little organisms that are everywhere around us. We can’t see them without a microscope because they are so small‚ but they are in the air‚ on our skin‚ in our bodies‚ in the ground‚ and all throughout nature. Bacteria are single-celled microorganisms. Their cell structure is unique in that they don’t have a nucleus and most bacteria have cell walls similar to plant cells. They come in all sorts of shapes including rods‚ spirals‚ and spheres. Some bacteria
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Are Viruses Alive? 1. Describe how a virus differs from bacteria. Most Viruses are harmful and their only purpose is to invade a host cell‚ reproduce and destroy the host cell in the process. Close to 99% of bacteria are beneficial with only a few that cause diseases. Life on earth could not exist without bacteria. Bacteria produce nearly half of the oxygen found in the atmosphere. Viruses have no cells‚ nucleus‚ cytoplasm or cell membrane. Viruses consist of tiny particles of nucleic acid
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infected with the S bacteria grew sick‚ and died‚ while mice infected with the R bacteria were not harmed. To determine whether the capsule on the S bacteria were causing the mice to die‚ Griffith injected the mice with dead S bacteria. The mice remained healthy. Griffith then prepared a vaccine of weakened S bacteria by raising their temperature to a point at which the bacteria were “heat-killed” meaning that they could no longer reproduce ( the capsule remained on the bacteria). When Griffith
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of the Pacific‚ Stockton Abstract The bacteria Sulfolobus Oileatacus has an enzyme called the Devastator‚ DevA‚ that helps metabolize crude oil. Since the bacterium is a thermophile‚ it won’t survive at normal temperatures and cannot be used to clean up oil spills. The devA gene was cloned through the polymerase chain reaction and was confirmed to be present using a restriction enzyme digest; after which the cloned genes were inserted into the bacteria E. coli through the transformation process
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antibiotic concentration decreases bacterial growth. Scientific Theory Bacteria are prokaryotes and can be identified by their shape. Spherical bacteria are called cocci‚ rod shaped bacteria are called bacilli‚ spiral shaped bacteria are called spirilla and curved shape bacteria are called vibrio. All bacteria possess a cell wall‚ cell membrane and cytoplasm‚ but no defined nucleus and no nuclear membrane. Some bacteria may contain other structures such as mesosomes‚ pili‚ photosynthetic membranes
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Predicting the Growth of Microorganisms Abstract Bacteria have been around us all the time just that not are bad there are also good bacteria. Throughout the session the bacteria changed in shape and how large it grew in many different ways. There were many different results in every bacteria that was examined‚ no bacteria looked alike towards one another. The bacteria in order to be produced it need to be put nutrient agar that would nourish it. The bacteria were inoculated into the nutrient agar so it
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already know about any organism and any laboratory procedure to the difficult task at hand. It is possible to identify a mixed culture by running familiar experiments on the unknown bacteria and taking information already known about specific bacteria and applying it to the results. This helps to slowly eliminate any bacteria that do not correspond with the results. I obtained tube number twelve to run various tests on. There are only two microorganisms‚ one is Gram positive and one is Gram negative‚
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Gram-negative and Gram-positive bacteria 2. Author: Nick Fiore‚ University of Kansas‚ Biology 402‚ Fall 2014 3:00pm room 6040 3. Abstract: The purpose of this experiment was to isolate two unknown bacteria and perform a series of selective and differential tests to correctly identify each. After the bacteria was isolated a series of differential and selective tests following the dichotomous key attached were used to identify each bacteria. The Gram-positive bacteria were identified as Staphylococcus
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