The Effect of Acetyltransferases on 2 different strains of E. coli Introduction Scientists have recently discovered that resistance to antibiotics may not be such a new thing. Evidence of bacteria samples in Canadian permafrost proposes that these resistances have been around for at least 30‚000 years (Luiggi 2011). In our required pre-lab reading‚ we learned tuberculosis is becoming increasingly drug-resistant‚ giving proof that bacteria can adapt to necessary changes in order to survive (Barry
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Abstract:Conjugation is a natural occurring process that involves the transfer of DNA from one cell into another through a physical connection between the cells. In the following experiment‚ two strains of Escherichia coli bacterial cells (donor F’lac+strs and recipient F-lac-strr) underwent conjugation to produce a transconjugant strain (F’lac+strr). MAC plates and streptomycin were utilized to determine if conjugation had occurred. When plated‚ the donor colonies appeared red and the recipient
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price‚ and high degree of reduction have made glycerol a highly attractive and exploited carbon source for the production of fuels and reduced chemicals. Here we report the quantitative analysis of the fermentative metabolism of glycerol in Escherichia coli through the use of kinetic modeling and metabolic control analysis (MCA) to gain a better understanding of glycerol fermentation and identify key targets for genetic manipulation that could enhance product synthesis. The kinetics of glycerol fermentation
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Material and methods Hosts ‚ plasmids and chemicals E. coli TOP10 strain was used for cloning and proliferation. E.coli BL21 DE3 was used as expression host cell. The pET 28a(+) plasmid was employed for gene expression. All chemicals were obtained from Merck Company (Germany). Codon optimization and gene synthesis Sequence encoding V-domain was obtained from Swiss-port‚ Uniprot KB and National Center for Biotechnology Information (NCBI) databases. 6x His-tag sequence was placed at N-terminal
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Jennifer Hauss March 4‚ 2015 Bacterial Transformation Lab Report Introduction In this lab‚ the goal was to transform the bacteria e-coli to glow in the dark (or under a black light). Four plates were set up with agar in them for the bacteria to feed on and grow. Changes were then made to the bacteria. One plate was the control plate‚ having only the LB or agar for the bacteria and negative pGLO‚ which is the liquid not containing the plasmid. This is the plate that was compared with the three
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of Shiga toxin producing Escherichia coli O104: H4 outbreak‚ Germany 2011 The Escherichia coli O104: H4 outbreak of 2011 originated in northern Germany and lasted from May through to June of the same year. “The outbreak was characterized by several unusual features: a high incidence in adults (especially women)‚ a greatly increased incidence of the haemolytic–Uraemic syndrome (in approximately 25% of patients‚ as compared with 1 to 15% in previous outbreaks of Shiga-toxin–producing E.coli)‚ a predominance
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Optimal Growth Conditions and Generation Time for E. coli Introduction The goals of these exercises were to determine the optimal growing condition and the generation time of E. coli. Materials and Methods Please refer to the lab manual for a complete list of materials. To determine the optimal growth condition for E. coli‚ we had to test the environmental factors that could affect the growth. We tested the relative growth of E. coli in the presence and in the absence of oxygen. The results
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HAT 1 Task 3 A. Analyze the selected communicable disease outbreak by doing the following: Describe the communicable disease outbreak. Avian Influenza‚ also known as the Bird Flu‚ is an influenza A virus caused by avian‚ which means bird. These viruses occur naturally in some birds. Wild birds in all areas of the world get the viral infections in their intestines‚ but do not usually become ill from them. The avian influenza is quite contagious among birds and can even kill domesticated
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Catherine went to live with her aunt‚ Catherine Ray Greene. 19-year-old Catherine married Nathanael Greene on July 20‚ 1774‚ in Greenwich‚ Rhode Island after they had met at one of her aunt parties. Nathaniel was 14 years older than her at the time. Following their wedding‚ the couple moved to Nathanael Greene’s home in Coventry‚ Rhode Island‚ where they spent less than a year together before he was called into service. In the fall of 1785 the Greenes left their family and friends in Rhode Island and
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Graham Greene: A Catholic Novelist Henry Graham Greene (1904-1991) is famous around the world as a prolific writer. During his career‚ extending over sixty years‚ he wrote more than twenty novels‚ a collection of short stories‚ several plays‚ screenplays as well as books of travel writing‚ essays and film criticisms. It’s not an easy matter to deal with such a milestone in English literature that came through so many different periods and matured so much over the time[1]. One aspect of his personality
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