2014 Biology 1 Cellular Processes Lab Section 903 Tianna Clarke Materials and Methods Part I – Restriction Enzyme Digestion To begin this experiment‚ the DNA molecules must be cut into smaller fragments with distinct enzymes called Restriction Enzymes through a process called Restriction Enzyme Digestion. Four microtest tubes were labeled 1 through 4 and added 10 µl of Enzyme Reaction Buffer to each of the four reaction tubes using a micropipette. DNA‚ and Enzyme 1 and 2‚ were then added to the
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Lab Report 3 In this lab‚ we will take a trip to the planetarium lab and will learn about Right ascension and declination‚ and altitude and zenith. After looking at the different points shown‚ we will log the altitude and zenith in the chart in our lab manual. Now we will look at the same points and label the right ascension and declination. Then we will learn about the easiest way to locate the star Polaris. As we started the first program‚ we answered a few questions to make sure we knew
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The Effect of pH on the Rate of Enzyme Catalysis of Catalase Objectives: The objective of this lab was to develop a protocol to investigate the effect of an environmental variable on the catalytic function of an enzyme. More specifically‚ the objective was to perform an experiment in order to test the effect of pH on the function of the enzyme catalase. Introduction: Enzymes are proteins that act as catalysts for reactions. This simply means that enzymes lower the activation energy required
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This lab is adapted from the canned denatured pineapple experiment. Instead of investigating the effect of canning on enzyme activity‚ we look for the presence of the substrate. This is a useful teaching lab for all the criteria and requires very few resources. Background Information: Gelatin is made from a protein called collagen which comes from the joints of animals. Gelatin may be dissolved in hot water. As the dissolved gelatin mixture cools‚ the collagen forms into a matrix that
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This lab report will be detailing the steps taken and the results discovered when using spectrophotometry to determine the percentage of copper in a copper-clad penny and the thicknes of the copper layer on the copper-clad penny. After 1982‚ copper coating has been used in the creation of the penny because the cost of pure copper has increase to the point that the amount needed t omake a penny cost far more than the actual value of the penny. This lab allowed us to see just how much copper coating
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Effect of enzyme concentration on rate of reaction Research question: is there a relationship between catalase concentration and the rate of reaction? Hypotheses: if the enzyme concentration is increased then the rate of reaction will increase. If the enzyme concentration is decreased then the rate of reaction will decrease. Controlled: temperature Independent: % catalyze concentration ( Dependent: rate of reaction (mm/s) Materials: Mortar and pestle Graduated cylinder Distilled
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MEC 325/580 Lab Report CNC Machining Lab Report Date: March 18‚ 2013 Group Leader: Jian Wu Team Members: Group Number: G4 Regment No.: D Insturctor: CONTENTS 1.Objective and introduction. 2.Principles and practice. 3.Design and result. 4.Conclusion and recommendations. Appendices 1. Objective and introduction * Objectives Operate the CNC milling machine using G-codes‚ M-codes in a CNC program. * Equipment: Minitech CNC machine (Series 2) * Machine
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LAB REPORT Introduction: In a chemical change‚ the identities of substances change and new substances form. In an equation the substances on the left are the reactants. The substances on the right are the products. In this experiment the objectives are to observe evidence that a chemical change has taken place. Infer from observations that a new substance has been formed. Identify and record observations that show energy is involved in chemical change observe the color‚ solubility of some substances
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Lab Report: Stoichiometry Lab Oct. 27‚ 2011 Claire Elizabeth Lab Partners: Hannah Signature:___________________ Introduction- Baking soda and vinegar are two common materials found in almost every household. That‚ plus the fact that all the starting and finishing materials are non hazardous and safe‚ is why this is one of the first chemical reactions that many people are exposed to The purpose of this experiment tests which of the two reactants (vinegar and baking soda) is the limited
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Microbiology-2460 Lab-003 March 31‚2008 Lab Report-Escherichia coli Abstract The purpose for this lab report was to identify and inform of an unknown bacteria that has been causing a patient to have lower abdominal and pelvic pain. To obtain the identification of this unknown bacterium‚ several biochemical tests needed to be performed in order to prescribe the correct medication to treat and cure the symptoms. Introduction In a lab today‚ I am to identify an unknown bacterium that is
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