The main goal of this experiment was to successfully clone human insulin cDNA into an expression vector. The gel electrophoresis results confirm that the inserts were successfully cloned into the expression vector based on the identical fragment position of each cut candidate in the gel. The fragment sizes of the both cut candidates produced on the gel when compared to the DNA ladder were larger‚ at roughly about 6000 base pairs and 6800 base pairs‚ than the predicted size of 2315 and 3119 base pairs
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Acid-Base Titrations A Titration of Potassium Hydroxide Phthalate and Sodium Hydroxide Hanna Piper Department of Chemistry‚ SUNY College at Brockport‚ Brockport‚ NY 14420 Chemistry 205.06 Abstract Titrations are used to find the molarity of an unknown solution. A titration begins with an analyte and titrant being used to measure the unknown molarity of the analyte. In the following experiment‚ sodium hydroxide was used as the titrant and potassium hydroxide phthalate was used as the
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Bioresource Technology 79 (2001) 207±225 Review paper The production of poly-(c-glutamic acid) from microorganisms and its various applications Ing-Lung Shih *‚ Yi-Tsong Van Department of Environmental Engineering‚ Da-Yeh University‚ 112 Shan-Jiau Road‚ Da-Tsuen‚ Chang-Hwa 51505‚ Taiwan‚ ROC Accepted 9 April 2001 Abstract This review article deals with the chemistry and biosynthesis of poly-(c-glutamic acid) (c-PGA) produced by various strains of Bacillus. Potential applications of c-PGA as
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The movie Juno is fiction and came out in 2007. It is about Juno MacGuff a 16 year old high schooler who gets pregnant after her first time having intercourse with Paulie Bleeker‚ who is also in high school. Although 9 years old‚ this movie is still extremely relevant in today’s society in regards to teen pregnancy. It tackles all the different options one can take and how emotionally‚ physically‚ and mentally challenge this decision is‚ especially when you’re a teen. Psychologist David Elkind theorized
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purpose of a synthesis essay is to make insightful connections. Those connections can show the relationship(s) between parts of a work or even between two or more works. It is your job to explain why those relationships are important. In order to write a successful synthesis essay‚ you must gather research on your chosen topic‚ discover meaningful connections throughout your research‚ and develop a unique and interesting argument or perspective. A synthesis is not a summary. A synthesis is an opportunity
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final compound which I am going to look at is 4-aminobenzenecarboxylic acid. The compound is also known as 4-Aminobenzoic acid. The molecular and structural formula of the compound is C7H7NO2 which means that the compound consists of seven carbon atoms attached to seven hydrogen atoms along with one nitrogen atom and two carbon atoms attached as a carbon-oxygen double bond. The displayed formula for the 4-aminobenzenecarboxylic acid compound is shown and it shows the formula in a ring form therefore
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Acids and Bases Shane Purpose- To study the relationship between pH and metabolism. 10/21/13 Materials- see attached Per. D Biology Procedure- see attached Data and Results- see attached Theory- PH stands for the power of hydrogen. People refer to the pH scale as a tool for finding out if a substance is acidic‚ basic‚ or neutral. This scale goes from zero to fourteen. The number seven is neutral. Any number higher than seven on this scale is basic. Any number lower is acidic
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A SEMINAR ON “SYNTHESIS OF LOW COST ADHESIVES FROM PULP & PAPER INDUSTRY WASTE” For the partial fulfillment of degree of Bachelor of Technology Submitted by DINESH G. TEMBHURNE (20090557) Under the guidance of MISS. NAZLI GITE. DEPARTMENT OF PETROCHEMICAL ENGINEERING DR. BABASAHEB AMBEDKAR TECHNOLOGICAL UNIVERSITY‚ LONERE (RAIGAD) - 402 103 2010-2011 DR. BABASAHEB AMBEDKAR TECHNOLOGICAL UNIVERSITY LONERE - 402 103‚ TAL – MANGAON
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alcohol (1.0 mL) and excess glacial acetic acid(1.5 mL‚ 17.6M) were eppendorf pipetted into a 5 mL conical vile. This mixture was acid catalyzed by the addition on two drops of H2SO4. The mixture was then heated to 150-160⁰C in order to increase energy for esterification to occur over a period of 60- 75 minutes. The mixture then was cooled to room temperature before the addition of 5% aqueous sodium bicarbonate( 1.0 mL) in order to react excess acetic acid to form a the anionic form sodium acetate
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ACCOUNTING 505 SYNTHESIS |Key Features of a Synthesis | (1) It accurately reports information from the sources using different phrases and sentences; (2) It is organized in such a way that readers can immediately see where the information from the sources overlap;. (3) It makes sense of the sources and helps the reader understand them in greater depth. |The Background
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