Introduction The purpose of this experiment is to synthesize methyl nitrobenzoate from methyl benzoate‚ concentrated HNO3‚ and concentrated H2SO4 via an electrophilic aromatic substitution reaction. Reaction Procedures/ Observations Use a 50 ml beaker to cool about 6 ml of concentrated sulfuric acid in an ice water bath. Weigh the vial containing about 3 grams of methyl benzoate and add it to the cooled sulfuric acid. Next pour about 2 ml of sulfuric acid to the nitric acid in the vial
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The data we collected and the calculations we performed demonstrated that the color of a given food dye depended on which light was transmitted in the visible spectrum and which light was absorbed. A quantitative measurement of the absorbance spectrum of each food dye was obtained through spectrophotometry.1 Through this method‚ we determined that the wavelength of maximum absorbance of a given food dye was directly correlated to the color of that food dye. Further‚ darker food dyes absorbed more
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The objective of these labs were to use sophisticated techniques to produce double-stranded RNA that was incubated with live Drosophila cells to inhibit the expression of our two genes of interest. The overall process of the four labs was to isolate and amplify DNA using the polymerase chain reaction with primers that contained gene-specific sequences to Thread or Dynamin-related protein 1 (drp-1)‚ along with T7 promoter site sequences. The amplified DNA was purified and both strands of the DNA
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Abstract: This experiment synthesized H-Gly-Phe-OH dipeptide using “Fmoc chemistry”. The first part of experiment was the synthesis of L-phenylalanine methyl ester hydrochloride. The methyl ester can be synthesised by reaction of thionyl chloride‚ SOCl2 and dry methanol with L-phenylalanine under reflux condition. The peptide bond was formed later in the experiment‚ where HBTU‚ DiPEA and a solution of Fmoc-Gly-OH in DMF were added to a solution of L-phenylalanine methyl ester hydrochloride in DMF
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Activity 1.7.2 Synthesis of Esters Purpose- the Purpose of this lab is to synthesize Esters by combining Carboxylic Acid and Alcohols. In this lab we will synthesize and then detect the odour of Esters formed. Materials- Materials that will be used in this lab are as follows:- Ethanol 7. Acetic Acid Eye Protection 11. Test tube rack Procedure- Prepare a hot-water by half filling a 500-mL beaker with water and heating it carefully on a hot plate until it comes to a gentle
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2.4.General procedure for the synthesis of 2‚3-dihydriquinazolin-4(1H) ones derivatives: A stirred mixture of isatoic anhydride (1 mmol)‚ amines containing 2-aminobenzothiazole or 2-amino benzimidazole (1.1mmol)‚ aromatic aldehydes (1 mmol) and 0.045 g of H-ZSM-5 nanozeolite was reacted in an oil bath at 100 oC for the appropriated times. Completion of the reaction was indicated by TLC. After completion of reaction‚ the mixture was cooled to room temperature. The solid residue was dissolved in hot
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Summary of Presentation: Fe2+/H2O- mediated synthesis of Melanins: Cases of Homogentisic acid‚ serotonin‚ and norepinephrine The powerpoint presentation that I attended was titled‚ “Fe2+/H2O- mediated synthesis of Melanins: Cases of Homogentisic acid‚ serotonin‚ and norepinephrine‚” the research covered melanin which is a dark pigmentation that can cause other complication and pathological conditions such as‚ vitiligo and ochronosis. It is believed that melanin protects from ultraviolet light rays
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Laboratory Exercise #3 Measuring Protein in Solution Abstract The purpose of this lab was to learn about the Biuret assay reaction to determine if it can detect proteins and amino acids; also‚ to understand the process of “salting out” proteins and how to determine the amount of protein in a solution. In order to do so‚ egg white and ammonium sulfate were mixed on ice and then put into the centrifuge. After PBS was added‚ the amount of protein could then be determined. After that‚ 14 test tubes
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like shampoo‚ liquid soap and bubble bath. Environmental Working Group’s analysis suggests that 97 percent of hair relaxers‚ 57 percent of baby soaps and 22 percent of all products in Skin Deep may be contaminated with 1‚4-dioxane [1]. Independent lab tests co-released by the Campaign for Safe Cosmetics in 2007 showed that popular brands of children’s bubble bath and body wash contained 1‚ 4-dioxane HEALTH CONCERNS:
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According to Figure 4 and 5‚ the moles of Cu initially obtained and at completion‚ differentiated. It was evident that the initial moles of Cu (0.0254)‚ did not regenerate all the amount‚ as 0.0124 moles of Cu was attained. In regards to this‚ the no. moles that was eliminated was approximately‚ 0.013. Respectively‚ in Figure 3‚ a large deviation amongst the initial and final quantity of copper‚ this implies that the rest of the mass that had diminished‚ was greater than the final product. These
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