agar plates To distinguish the growth characteristics of microorganisms in various differential‚ and selective media. Differentiate bacteria based on their ability to hydrolyze starch. Materials: Plates of EMB‚ Starch and blood agar. Stool sample. Inoculating loop. Bunsen burner. Soil sample. Cotton soap. Skin sample. Gram iodine. Results: Starch agar: Special types of media: Type of medium Medium Appearance of medium and growth Selective and differential Eosin-methylene
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Microbiology Module 02 Homework Assignment Use the information presented in this module along with additional outside research to answer the questions: 1) Compare and contrast prokaryotic and eukaryotic. a) Prokaryotes and eukaryotes are two types of cells that are very different but share some certain properties such as methods of reproduction‚ protein synthesis‚ an organized metabolism‚ response to stimuli‚ and plasma membranes. One significant difference is that prokaryotes are without a cell
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Unknown #11: Citrobacter freundii Tryptic Soy Agar Test (TSA): TSA is a basic medium that is most similar to nutrient agar. The agar contains carbon‚ nitrogen‚ sodium chloride‚ and agar. This allows for the growth of a large variety of microorganisms to grow and ferment in the medium. Mannitol Salt Agar (MSA): MSA is a selective and differential medium that favors the growth of salt loving organisms. It is commonly used to distinguish the different species of Staphylococci. If an organism ferments
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McConkey) agar is a culture medium designed to grow Gram-negative bacteria and stain them for lactose fermentation. It contains bile salts (to inhibit most Grampositive bacteria‚ except Enterococcus and some species of Staphylococcus)‚ crystal violet dye (which also inhibits certain Gram-positive bacteria)‚ neutral red dye (which stains microbes fermenting lactose)‚ lactose and peptone. QUALITY CONTROL Results after 24 hrs at 35º C Organisms ATCC Growth Colour Escherichia coli
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IDENTIFYING GRAM POSITIVE COCCI As mentioned in Exercise 8‚ “Identifying Gram Negative Rods”‚ identifying bacteria is a common activity in the microbiology lab. Like the game Clue™‚ each time you gather a piece of information to solve the mystery‚ you gather some information that supports some identities and eliminates others from contention. In the lab‚ the process continues as you gather more information until only one microbe remains and all others have been eliminated as possibilities. Thus
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OBJECTIVE: 1. To distinguish the bacteria abilities to metabolize various substrates and end products formed. 2. To observe the growth of different bacteria species in term of structures and its morphology based on different chemical substance applied. 3. To observe physiological and immunological properties utilized by different species of bacteria. INTRODUCTION: Bacteria biochemical testing can determine the types and numbers in terms of colony forming units of bacteria present in a
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the mannitol salt were used to incubate a TSB broth to grow the gram-‐positive culture. The purity of this broth was tested using gram-‐staining technique. A circular colony from the TSA plate was used to incubate a TSB broth for gram-‐negative growth. Similarly‚ examining the morphology of the bacteria-‐using gram staining technique tested the purity of the both. After the isolation of gram-‐positive and gram-‐negative bacteria from unknown A‚ specific biochemical tests
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counts the number of colonies produced by a very dilute suspension of bacteria on an agar plate and to observe the differential staining behaviour of the living bacteria. This involves counting the colonies produced by viable cells under favourable growth conditions. Some techniques needed before the viable count‚ like pour plate method‚ spread plate method and most probable number method. The viable count is very specidic‚ as it represents the number of colony forming units (/g) or (/ml) of the sample
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........................................................................................1 History of Microbiology and Culture Media ...................................................................................................3 Microorganism Growth Requirements .............................................................................................................4 Functional Types of Culture Media
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(1.0%‚ 1.5%‚ and 2.0%) of Hughes‚ et. al. (1958) Mineral Medium No. II. After ten (10) days of culture‚ the Trichome Length (TL)‚ Trichome Width (TW)‚ Sheath Width (SW) and Total Length of the Lyngbya filaments were measured from photomicrographs of the samples using Image Tool (Version 3.00) developed by the University of Texas Health Science in San Antonio (UTHSCSA). Lyngbya filaments in all cultures enriched with the Hughes medium obtained significantly higher TL and TW over those of the
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