LAB Report #3 Introduction: In this lab we have focus on Isolation of bacteria from environment. Microorganisms are found throughout the environment: in the air and water; on the surface of any object such as clothes‚ walls‚ furniture; in soil and dust; and on and in our own bodies (skin and mucous membranes). In order to demonstrate the ubiquity and diversity of microbes in the environment‚ samples from immediate areas of the environment and/or from your body will be obtained and cultured
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curriculum is easily adaptable to accommodate any number of students. In this lab‚ students identify an unknown bacteria using a biochemical method and a molecular method. For the biochemical method‚ students use a combination of differential growth tests and enzyme tests developed for clinical use. For the molecular method‚ students PCR amplify and sequence the 16S rRNA gene from their bacteria‚ then use BLAST to search the bacterial database and identify the species that most closely matches
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plates were examined for bacterial growth of two different colonies. On a Nutrient agar plate two different cultures were observed. In order to proceed identification‚ those cultures were isolated as separate organisms‚ using inoculating streak technique and incubated until the next class period. The expected result was supposed to show Gram positive and Gram negative bacterium growing on a separate media. Having checked two Nutrient plates‚ signs of visible growth of two different bacterium were present
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Introduction: Antimicrobial agents are chemicals that are used against bacteria. There are many such agents available. Because there are many different situations where bacterial control is important‚ no antimicrobial agent is effective in all situations. For example‚ you wouldn’t use the same compound to fight an ear infection as you would use to sterilize surfaces in an Operating room. The situations are completely different. In one case‚ you are trying to assist the body to fight off an internal
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CORE BIOLOGY PRACTICALS You will need to know these practicals as the exam board may ask you questions based on them. Below is a summary of each one. Name of practical and independent & dependent variables Effect of caffeine on Daphnia heart rate Independent: caffeine concentration Dependent: heart rate of Daphnia Measuring the content of Vitamin C in fruit juice Independent: fruit juice Dependent: volume of juice required to decolourise 1cm3 of DCPIP The effect of temperature on cell membranes
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The first test I did was the catalase test. It had a positive reaction meaning the bacteria produced the enzyme catalase. This helped me narrow my unknown down to Micrococcus and Staphylococcus. I inoculated my unknown onto a mannitol salt agar. The growth and color change to yellow indicated mannitol fermentation because acid was produced. The acid fermentation is a characteristic of the pathogen
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Introduction In my report I will discuss what I did as an experiment and what I hope for it to achieve. Firstly I carried out an experiment to assay the effectiveness that a range of disinfectants have on the growth of ecoli and whether or not it can prevent it from growing. From the experiment i should be able to see that some disinfectants have a greater effect than others do. From this I shall then draw a conclusion and evaluation on what was the most effective‚ and could there have been any
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Connor Lauffenburger 3/17/13 pGlo Transformation Lab Report I Introduction The purpose of this experiment was to show the genetic transformation of E. coli bacteria with a plasmid that codes for Green Fluorescent Protein (GFP) and contains a gene regulatory system that confers ampicillin resistance. A plasmid is a genetic structure in a cell that can replicate independently of chromosomes. In this lab‚ the Green Fluorescent Protein‚ which is typically found in the bioluminescent jellyfish Aequorea
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their products. Very few of the things we eat or drink are bacteria free. They merely have greatly reduced numbers of “harmless” bacteria. It is often necessary to determine how many live bacteria are actually in a sample‚ especially when measuring growth rates or determining disinfectant effectiveness. This involves MacConkey agar which is a selective and differential
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The Results from the Gram positive tests indicated that the unknown #4 was Streptococcus pyogenes. All seven tests on the unknown matched S. pyogenes perfectly. The blood agar plate proved the unknown to be β hemolytic‚ meaning the unknown bacteria was capable of complete hemolysis. This test separated the unknown into the β Streptococcus group‚ narrowing the possible bacteria to S. aureus or S. pyogenes. The Catalase test was used to determine if the unknown could break down hydrogen peroxide
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