Biochemistry Lecture Exam 1 Study Guide Chapter 1: Biochemistry: An Introduction I. Introduction a. Biochemistry: the study of molecules and chemical reactions of life b. Divided into 3 principal areas: 1. Structural chemistry of components of living matter & relationship of biological function to chemical structure 2. Metabolism – total chem reactions that occur in living matter; building & breaking down of molecules 3. Chemistry of processes & substances that store & transmit biological
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BCH 369L–Biochemistry Laboratory Fall 2016 Module C Orlando Martinez uteid: olm298 Introduction The purpose of this lab is to implement the technique of gel electrophoresis in the purification and size determination of various proteins and DNA fragments. In order to do this‚ a polyacrylamide gel will be prepared and placed in a buffer-containing gel electrophoresis apparatus. Next‚ an aliquot of acid phosphatase and a molecular weight marker (Composed of Phosphorylase B‚ bovine serum albumin‚ ovalbumin
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Unit 1: Biochemistry Review Chemical Bonding Three types of bonds Ionic bond: 2 oppositely charged atoms (Na and Cl) Polar covalent: Unequally shared electrons (H and O) Non polar covalent: Equally shared electrons (C and C) or similar electronegativity Electronegativity and chemical bonding Electronegativity is a measure of the strength in which an atom can attract electrons. A difference of < 0. 5 is non polar covalent bond A difference of 0.5 – 1.7 is polar covalent bond A difference of > 1
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Endocrine System: Biochemistry‚ Secretion and Transport of Hormones 1. Place the following hormones into one of the three categories of hormones (peptides‚ amines or steroids): T4 (thyroxin)‚ estradiol‚ norepinephrine‚ insulin‚ aldosterone‚ glucagon‚ cortisol‚ growth hormone‚ T3 (triiodothyronine)‚ epinephrine‚ testosterone and vasopressin (ADH). |Peptides |Amines |Steroids | |Insulin
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Biochemistry of Proteins; Isolation of Ovalbumin and Enumeration of thiol groups Abstract Thiol groups are important to protein folding and forming disulphide bonds that are essential to protein structure. Determining the number of thiol groups in a protein is important in determining the tertiary structure of the protein. The ovalbumin is the experiment was purified from egg white using centrifugation and ammonium sulphate precipitation and then the thiol groups identified using DTNB and spectroscopy
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Chemistry of Life Practical Investigation | Investigation of a Factor Affecting Enzyme Action | By: Jerry Li 02/03/2013 02/03/2013 | | HL Biology Term 1 Year 11 HL Biology H Contents 1 Design 2 1.1 Defining the problem 2 Research Question 2 Hypothesis 2 Background Information 2 Investigation Variables 3 1.2 Controlling Variables
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Chapter 7: Carbohydrates I. Carbohydrates A. Most abundant organic molecule in nature a) 3 major classes: Monosaccharides – simple sugar – polyhydroxy aldehyde or ketone; represent individual sugar Oligosaccharides – consist of short chains of monosaccharide units joined together by covalent bonds; 2-10 strings of sugar Polysaccharides – long chains having hundreds or thousands of monosaccharide units b) Functions: Provide energy thru oxidation – glucose oxidized for energy Supply carbon
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AbstractWater is important substance for all living organisms. The physical and chemical properties of water play a central role in biological structure and function of the organism. The ionization ability of water to form H+ and OH- ions make it very unique. The hydrogen-ion concentration of biological system is usually preferred as the pH system‚ which determines the pH level of dilute aqueous solutions. In this laboratory‚ the data collected from the experiment will be used to graph the titration
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BASIC CONCEPTS IN BIOCHEMISTRY A STUDENT’S SURVIVAL GUIDE Second Edition HIRAM F. GILBERT‚ Ph.D. Professor of Biochemistry Baylor College of Medicine Houston‚ Texas McGraw-Hill Health Professions Division New York St. Louis San Francisco Auckland Bogotá Caracas Lisbon London Madrid Mexico City Milan Montreal New Delhi San Juan Singapore Sydney Tokyo Toronto • • • • • • • • • • • • BASIC CONCEPTS IN BIOCHEMISTRY‚ 2/E Copyright © 2000‚ 1992 by the McGraw-Hill Companies‚ Inc. All rights
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Introduction Methionine and cysteine are both sulphur containing amino acids. Most proteins will contain one‚ or both of them at some point in the polypeptide chain. As such‚ many amino acids contain sulphur in some form‚ which is required in small amounts in the mammalian diet. Methionine has a thioether side chain‚ and cysteine’s contains a thiol group. These side chains exist as free thiols inside the cell‚ and are oxidised causing them to pair up and form disulphide bonds in an extracellular
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