1) A peptide‚ KKDSRHSTTR is tightly bound to a negatively charged ion exchange column. The column is washed with a pH 7.2 HEPES buffer (50 mM) and the peptide does not elute. Suggest two ways that you can change the buffer that will make the peptide elute from the column. 2) What amino acid(s) is/are involved in crosslinking polypeptide chains. How can these crosslinks be cleaved and prevented from reforming? 3) A polypeptide is subjected to the following degradative techniques results
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RNA Analysis 1. The color result is light brown. 2. A positive Biuret test indicates the presence of proteins in the filtrate. Biuret is used to test for proteins. It is a light blue color and changes to purple in the presence of protein‚ a polymer made of amino acids. It will not change color when only amino acids are present. 3. What type of molecules present in the suspension is hydrolyzed by the mild acid treatment? 4. The products of hydrolysis are peptones‚ peptides‚ and amino acids
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I. Changes Leading to Exploration A. Expulsion of Moors in Spain and reunification of the country under Catholic rulers Isabella and Ferdinand caused an age of exploration and expansion. B. After German Monk Martin Luther posted his 95 theses against church abuses‚ there was a powerful movement away from the Catholic Church called the Protestant Reformation‚ causing the Roman Catholic countries to secure their power in the world by converting
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Oct 2012 LSM 1101 Biochemistry of Biomolecules Class 1a: Nucleic acids Our class on DNA is divided into 3 parts: (I) Genetics (II) DNA structure (III) Concepts and applications. I. Genetics: In the primordial period‚ simple molecules were formed from atoms and from these molecules‚ macromolecules were formed. These macromolecules formed life and all living organisms. The classical genetic and heredity observations in the 19th century started the search for the origin of life. The transforming
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DNA and RNA Replication Deborah J Brooks Biochemistry (GRT1) Task 1 Western Governors University Objectives DNA Replication at Biochemical Level Role of Ligase Role of mRNA Role of RNA Polymerase Inhibition related to the death cap mushroom Introduction Nucleic acids are required for the storage and expression of genetic information. There are two chemically distinct types of nucleic acids. DNA (deoxyribonucleic acid). The repository of genetic information. RNA (ribonucleic acid)
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1. Receptors for touch‚ heat‚ pressure‚ vision‚ and hearing are classified as ___ because they sense stimuli that arise external to the body. 2. Color vision receptors are known as ________ . 3. The posterior pituitary gland secretes __ _ and ___ _. 4. The adult pineal gland is a small‚ shriveled remnant of the child’s. True False 5. In the blood hydrophillic hormones are often bound to transport proteins. True False True False 6. People
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Biological chemistry 1B Experiment 1: Thin layer chromatography [TLC] Aim The aim of this experiment is to apply the technique of TLC to first separate out a mixture of coloured dyes and then to do the same for selected range of amino acids. Procedure 2 MMs of each different colour were collected into separate beakers. Approximately 5 drops of water is added to each of the beakers containing the MMs and the tablets were stirred until
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Restriction Endonuclease Digestion of DNA from E. coli cells and Analysis by Agarose Gel Electrophoresis Introduction The main goals of this experiment are testing an alternative procedure called “boiling lysis”‚ evaluating the quality of the purified plasmid for restriction digests‚ and identifying the mislabeled plasmid. The plasmid DNA from a carrier E. coli strain was purified by the boiling lysis. In the boiling lysis method‚ the bacterial cells were given momentary heat treatment
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what I accomplish in the campus reflects my personality. I decided to study biochemistry because I want to make a change in this world‚ and also because I want to serve the humanity through harnessing the fields of biology and chemistry to ameliorate people’s health and to develop new technologies that will improve industrial processes. Possessing millions of dollars will support my post studies in chemistry and biochemistry branches‚ which will enhance my level of knowledge in these fields‚ thus having
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Unit 2 Biosynthesis and Degradation of Nucleotides Module Description: This module is designed to learn the biosynthesis of nucleotides 1 Topics (Chapter 22.4/25.1: Lehninger Principles of Biochemistry) 1. De Novo Purine Nucleotide Synthesis Begins with PRPP 2. Purine Nucleotide Biosynthesis Is Regulated by Feedback Inhibition 3. Pyrimidine Nucleotides Are Made from Aspartate‚ PRPP‚ and Carbamoyl Phosphate 4. Pyrimidine Nucleotide Biosynthesis Is Regulated by Feedback Inhibition 5. Nucleoside
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