Clays are one of the important natural materials‚ which have been examined for catalytic applications. Clays are characterized by a layered structure and hence they are slippery when wet. They are broadly classified as cationic or smectite type (having layer lattice structure in which two-dimensional oxyanions are separated by layers of hydrated cations) and anionic or brucite type (in which the charge on the layer and the gallery ion is reversed complimentary to smectite type). Anionic clays are
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CHEMISTRY 1184 Laboratory Manual for GENERAL CHEMISTRY I Jim Carroll Roger Hoburg Dana Richter-Egger August 2006 University of Nebraska at Omaha i Table of Contents Table of Contents ......................................................................................................................................... i Chem1184 SYLLABUS............................................................................................................................... ii Preliminary
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produce higher reactions. The last hypothesis is that enzyme reactions work best at a neutral pH. I observed and recorded the effect of these conditions on catalase. The enzyme catalase is important because it handles the decomposition of approximately half of generated H2O2 in living organisms [2]. It is important for biologists to understand catalase because all cells produce hydrogen peroxide‚ and its the job of catalase to break it down. This research will help increase the knowledge of enzyme
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Procedures for Part A: For Activity A‚ we first tested enzyme activity. First‚ we used an H2O2 syringe to transfer 10 mL of H2O2 into an unlabeled 60-mL cup. Then‚ we used a transfer pipet to add one mL of catalase solution into the unlabeled 60-mL cup that we put H2O2 in. After that‚ we observed the solution for one minute. Then we tested the effect of boiling on enzyme activity. First we used a transfer pipet to transfer 4 mL of catalase into a test tube. After that‚ we placed the test tube filled
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started to create bubbles‚ then it contained the catalase that could break down the hydrogen peroxide or H2O2. The reaction between liver and hydrogen peroxide or H2O2 was an exothermic reaction. After having reacted‚ the liver and hydrogen peroxide or H2O2 mixture caused the temperature to rise. When heat is being released from a reaction it is caused exothermic. In the body hydrogen peroxide or H2O2 is
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determined rate law is rate = K[I-].969 [H2O2].991 and the experimentally determined activation energy is 59.50 kJ/mole. Introduction: The rate of a reaction varies at different temperatures and reactant concentrations. In this experiment‚ the orders and dependence of the rate constant of the products used are determined by the following chemical reaction: 2I- + H2O2-1 + 2H+ -> I2 + 2H2O-2 The general rate law for this reaction is: Rate = k [I-]x [H2O2-]y where k is the rate constant‚ x and
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substrate concentration and inhibitors are other factors affecting enzyme functionality. The substrate in this lab is Hydrogen Peroxide (H2O2) and the enzyme is catalase in the liver‚ which locates in almost all living organisms and prevents the accumulation of toxic levels of hydrogen peroxide by decomposing hydrogen peroxide to water and oxygen. (2 H2O2 → 2 H2O + O2) Purpose To investigate and quantify an enzymatic reaction rate To observe how a number of variables affect the reaction rate
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would be the least amount of H2O2. The enzyme activity mirrored my predictions. Also‚ similar results occurred at the 50-second mark. Overall this experiment proved that substrate concentration decreased as time increased and eventually levels off when there is no more substrate to work on. Introduction: Catalase is
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Chemistry SCH3U Unit 1 Lesson 1 Key Questions 1. List three physical properties and one chemical property of a) an iron nail * conducts heat and electricity well * solid at room temperature * ductile * rusts in water (chemical) b) gasoline * liquid at 20deg C * boils between 20 and 200 degrees Celsius * not soluble in water * flammable in the presence of oxygen (chemical) 2. People in a workplace should have access to MSDS sheets so that * chemicals
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Enzyme Catalase Activity in Reaction with the Substrate Hydrogen Peroxide Abstract We performed these experiments to observe the effects of enzymes on the rate of reactions. We tested and compared the activity of the enzyme catalase on the substrate H2O2 in various states and percentages‚ and observed the absorption values of the enzyme-substrate relationship at different concentrations. Our results show that the more substrate available‚ the quicker the reaction will happen except in one test‚ which
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