A Quantitative Enzyme Study: CATALASE FlowChart Purpose: Measure the rate of enzyme activity in different conditions Procedure: A. Extraction of Catalase 1. Peel potato 2. Cut into cubes 3. Mass 50g 4. Measure out 50ml of cold distilled water in blender 5. Add crushed ice into blender (small amount) 6. Add the potato cubes into the blender 7. Turn on blender on high for 30 seconds 8. Prepare an ice bath - FROM THIS POINT ON PREPERATION MUST BE CARRIED OUT IN AN ICE BATH – 9
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reaction with respect to the Hydrogen Peroxide (H2O2)‚ Potassium Iodide (KI) and the Sulphuric Acid (H2SO4) by the use of an Iodine clock reaction. Calculate the rate constant‚ mechanism and equation Find the effects of temperature on the rate of reaction The effects of a catalyst on the rate of reaction Find the activation enthalpy (Ea) of the reaction‚ with and without a catalyst Background: Hydrogen Peroxide and Potassium Iodide equation: H2O2 + 2I - + 2H + I2 +
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1/3 dilution of H2O2‚ 10 mL of H2O2 and 20 mL of water. This beaker was left on the table in room temperature. The third beaker was labeled 37° and contained 1/3 dilution of H2O2‚ same as above. This beaker was placed in in a water bath set to 37° c to equilbriate. The fourth beaker was labeled 59° c and contained 1/3 dilution of H2O2. This beaker was placed in a water bath set to 59° c to equlibriate. The fifth beaker was labeled cold and contained a 1/3 dilution of H2O2. This beaker was
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Introduction to Biochemistry Biochemistry : a multidisciplinary science that explores the chemistry of living organisms and molecular basis for changes occurring in living cells. Principal areas of Biochemistry 1. Struture and Function of Biomolecules Based on structure depend the function (structure function relationship) Example: Hemoglobin S differs from regular adult hemoglobin (hemoglobin A) by just one single amino acid. A valine replaces a glutamine in the 6th position of the beta
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Bioelectrochemistry 74 (2008) 62–65 Contents lists available at ScienceDirect Bioelectrochemistry j o u r n a l h o m e p a g e : w w w. e l s ev i e r. c o m / l o c a t e / b i o e l e c h e m Short communication An electro-catalytic biosensor fabricated with Pt–Au nanoparticle-decorated titania nanotube array Qing Kang‚ Lixia Yang‚ Qingyun Cai ⁎ State Key Laboratory of Chemo/Biosensing and Chemometrics‚ Department of Chemistry‚ Hunan University‚ Changsha 410082‚ PR China A R
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Oxygen Requirements of Bacteria BACKGROUND The GasPak system is useful for culturing anaerobic bacteria on standard microbiological media because the GasPak generates carbon dioxide and hydrogen. The hydrogen will combine with oxygen present in an anaerobic jar to produce water. This system can reproducibly attain oxygen levels in the parts per million range if used correctly. This is the best method for determining the oxygen requirements of unknown organisms. A candle jar is useful
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lens solution‚ sinks and showers. It can cause a number of opportunistic infections including infections of the skin‚ external ear canal and of the eye. Nitrifying bacteria recycle organic nitrogenous materials from ammonium (the endpoint for the decomposition of proteins) to nitrates. Their presence
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graduated cylinder‚ pour twenty-five mLs of H2O2 into the 50 mL beaker 3.Gently place the beaker of H2O2 into the water bath; heat the H2O2 to thirty-seven degrees Centigrade‚ or ninety-eight point six degrees Fahrenheit 4.Once the solution is at the desired temperature‚ with the forceps‚ dip a filter-paper disk into the pureed liver and place on a paper towel to remove excess liquid 5.Using the forceps‚ place the filter-paper at the bottom of the H2O2 solution and release it. 6.Using the stopwatch
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flask. 5) Titrate the acidic iron (II) sample with KMnO4 (aq). 6) Repeat steps 4 and 5 until three consistent results are obtained within 0.1 mL. 7) Pipette 10.0 mL of H2O2 (aq) solution into a clean 250 mL Erlenmeyer flask. 8) Measure out 5.0 mL of H2SO4 (aq) solution and add it to the 10 mL H2O2 (aq) solution. 9) Titrate the acidic H2O2 (aq) solution with KMnO4 (aq). 10) Repeat steps 7-9 until three consistent results are obtained within 0.1 mL. 11) Dispose of solutions into waste buckets. Materials:
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To determine the effects of pH on catalase enzyme activity.Enzyme catalase Lab Statement of the problem : Catalase breaks down H2O2 (hydrogen peroxide) into water and oxygen. H2O2 is a toxic substance formed during metabolism. Hypothesis: To determine the effects of pH on catalase enzyme activity and as you increase the pH the more oxygen that will be produced. Materials: 1. 5 clean test tubes 2. HCI (acid) 3. Dilute HCI 4. Water 5. NaOH (base) Procedure: We start to fill
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