Práctica de La materia de Bioquímica I‚ tercer semestre de La Carrera de ing. Bioquímica‚ semestre 2009-2010. Practica 2. Enzimas: Catalasa y alfa-amilasa Dra. America Pech y Akè Enzimas: Catalasa y alfa-amilasa Objetivos General. Que el alumno conozca‚ comprenda y pueda observa y valorar la acción de las enzimas en los alimentos y pueda comprender cada uno de los conceptos relacionados con las enzimas como son la catalasa y alfa amilasa en las practicas a realizar en diferentes alimentos
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The purpose of this lab was to determine the effects of changes in variables such as temperature‚ pH and concentration on catalase enzyme activity. Proteins are organic compounds made up of amino acid subunits consisting of carboxyl and amino functional groups‚ linked together by peptide bonds through condensation reactions. They are an essential part of all living organisms. Enzymes are “biological catalyst used to speed up chemical reactions (Athanasopoulos‚ 2014). Normally‚ for chemical reactions;
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of the experiment‚ the effect of pH of the rate of peroxidase activity‚ and the effect of temperature on the rate of peroxidase activity. During the lab‚ the lab group tested 7 test tubes‚ including 1 blank‚ with different amounts of pH 5 buffer‚ H2O2‚ Peroxidase‚ and Guaiacol. After the certain amount of mL per substance was mixed‚ the absorbance readings for the effect of peroxidase concentration were taken from the spectrophotometer. The results for the effect
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Aims To investigate the effect of varying the concentration of H2O2 has on the reaction‚ and to determine the respective order of reaction. To investigate the effect of varying the concentration of KI has on the reaction‚ and to determine the respective order of reaction. To investigate the effect of varying the temperature has on the reaction‚ and to determine the respective order of reaction. To investigate the effect of introducing Ammonium Molybdate to the reaction Arrhenius stuff
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Independent | temperature | Celcius | 0‚ 21‚ 50‚ 80 | Dependant | Potato juice and MnO2 | Centimeters | 0~5 | Controlled variable | Units | Effects | Control By | Amount of H2O2 | mL | Amount of bubbles | Using syringe | Equipment Syringe Beaker Ice Hot Plate (Heating Pa) Test tubes Potato PH7 soap Thermometer H2O2 MnO2 (non-biological catalyst) Method 1. Prepare the potato juice by grinding or squeezing and pour it into a beaker. 2. Then pour a little of the soap in. This
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Kinetics Kinetics 6.1 Rates of reaction 6.2 Collision theory 6 16.1 Rate Expression (AHL) 16.2 Reaction mechanism (AHL) 16.3 Activation energy (AHL) 6.1 Rates of reaction 6.1.1 Define the term rate of reaction. 6.1.2 Describe suitable experimental procedures for measuring rates of reactions. 6.1.3 Analyse data from rate experiments. © IBO 2007 Figure 601 An explosion is a quick reaction D ifferent chemical reactions occur at different rates (i.e. speeds)
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Abstract: After reviewing the basics of enzymes and catalysis‚ we take a dive into the wonderful world of catalase. Beginning with establishing a base line of just how much hydrogen peroxide there is in 5.0mL of the reacted solution; to figuring out exactly how much actually reacted after 300 seconds of catalyzed reaction. Follow the experiment from the beginning steps right to the end as you see where the students went wrong‚ interpretation of the results‚ and great answers to work sheet
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AP Biology Sunday‚ October 24‚ 2010 Enzyme Lab Write-Up Purpose: To observe how an enzyme affect the speed of chemical reaction. To describe how the concentration of an enzyme affects its ability to work. Hypothesis: Depending on the concentration of the catalase which the disk is soaked in‚ it will have a direct correlation on the rate of hydrogen peroxide being broken down into oxygen gas. Prediction: Since the rate of reaction can be lowered by the addition of
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as qualitative tests for Product A (K2S2O8) in comparison with H2O2‚ confirm the presence of the peroxodisulfate anion and the identity of the individual yields. INTRODUCTION Electrolysis is a widely used technique in the large-scale preparation of several industrially important inorganic chemicals‚ one of which is potassium peroxodisufate. The S2O82- ion is one of the strongest known oxidizing agents‚ even stronger than H2O2‚ and will oxidize many elements to their highest oxidation states
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Y3O7-2 (basic solution) 7) Given the following unbalanced oxidation-reduction reaction that occurs in basic solution: S2O3-2(aq) + H2O2(aq) a) b) c) d) S3O6-2(aq) + SO4-2(aq) + H2O(l) Balance the reaction. Which substance is the oxidizing agent? What is the equivalent weight of H2O2 (Molar mass = 34.0 grams) in the above reaction? What is the normality of a 0.040 M H2O2 solution used in the above reaction? 8) Iodine reacts with thiosulphate ion (S2O3-2) in acidic solution to form iodide and tetrathionate
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