NA transformation of E. coli: The two plasmids were added to individual tubes containing E. coli and one with no plasmids. The three samples of E. coli were heated in a 42°C water bath for 90 seconds to heat shock the bacteria so that the plasmids would be taken up by the E. coli. These samples were then incubated at 30°C for half an hour and then plated on LB agar. Each tube was plated on an LB plate and a LB + ampicillin plate. Ampicillin is an antibiotic that is effective against E. coli‚ both
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Etymology The Black Nazarene derives its name from Jesus’ hailing from Nazareth in Galilee‚ and the image’s very dark complexion unusual among most Philippine religious images. Adorning the statue’s head are the traditional "Tres Potencias" ("three powers") halo‚ symbolizing the three powers of the Holy Trinity. The three rayos ("rays") are used to exclusively identify Christ in traditionalHispanic iconography‚ and are an angular evolution of the common cruciform halo. The importance of the holy relic lead
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Containerized 200 kW NAS Battery (to be released in 2015) 1. A “Plug & Play” 200 kW NAS Battery Unit (NBU) is under development at NGK. It will: ‐ Expedite shipping‚ installing and relocating multi‐MW NAS installations ‐ Accommodate a broad range of ambient site conditions: e.g. Operational temperature range is from ‐45 to 55C (special type) or –10 to 45C (standard type). Special type for salt‐air coastal environments is available. 2. A new 36 kW NAS module design is required for 200 kW NBUs
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Exploring the Personality Structure in the 11 Languages of South Africa Jan Alewyn Nel‚1 Velichko H. Valchev‚2 Sebastiaan Rothmann‚1 Fons J. R. van de Vijver‚1‚2 Deon Meiring‚3 and Gideon P. de Bruin4 1 2 North-West University‚ South Africa Tilburg University‚ The Netherlands 3 University of Pretoria‚ South Africa 4 University of Johannesburg‚ South Africa ABSTRACT The present study‚ part of the development of the South African Personality Inventory (SAPI)‚ explores the
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"Narcotics Anonymous is a nonprofit Fellowship or society of men and women for whom drugs had become a major problem. We are recovering addicts who meet regularly to help each other stay clean." After that‚ they directed the attention to a table with the NA book and other books and pamphlets which could be purchased or are for free. After the text was read‚ people celebrated special occasions. New members were welcomed greatly‚ as we had one at the time‚ and also a mother and son who came in later. All
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"MEMORIES" Nothing compares to having a family‚ a complete‚ happy family. I am proud to have had one. Unforgettable memories of a sweet childhood‚ growing up with pure love and affection‚ so many people wanted to have. I was nourished with hope‚ that someday I’ll have something more than I’ve expected. My parents gave me everything. They wanted me to have the best of endless possibilities. I owe my life to them. I am nothing without them. They are the reason why I have a fighting spirit‚ to finish
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Parent Involvement Parents play the most important role in teen pregnancy prevention. Healthy Futures for Texans (2010) states‚ “Teens continue to say that parents (46%) most influence their decisions about sex.” In most cases they are the teen’s first contact in learning what sex actually is. The fact of the matter is most parents aren’t educated well enough on the topic themselves and or are just afraid to speak well in depth on it. Based on a Planned Parenthood poll (2010)‚ “Ninety-three percent
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Jose Rizal (1861-1896) Rizal was born on June 19‚ 1861‚ in Calamba‚ Laguna‚ the seventh child and the second son of the 11 children of Francisco Mercado and Teodora Alonso. Rizal’s parents were not only well-to-do‚ but also well educated‚ a rarity among Filipino families then. His father‚ a sugar planter and landholder‚ attended a Latin school in his native Binan town‚ also in Laguna‚ and a college in Manila. His mother‚ who had a good business sense managed some small enterprise‚ also studies at
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SAAS MARKETING FOR CEOS HOW TO GO TO THE TOP OF THE MARKET and stay there TA BLE OF CO N T E N T S i 1. Introduction 5. The Story of Shopify 8. Going to Market 9. Know Your Customer 10. Create a Product Your Customers Will Love 11. Create a Company Your Employees Will Love 12. Support Your Customer at All Costs 13. Create a Great Customer Experience 13. Marketing Takeaways 14. Getting to the Top of the Market 16. Early Days - Growth Hacking 17. Inbound Marketing (Content‚ SEO and Social Media)
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The SAS Institute: The Best Company to Work For The SAS Institute is now known for being an ideal business with superb working conditions for employees and making a profit after its appearance and number one ranking on Forbe’s list of best companies to work for. The SAS Institute is the world’s largest privately held software company with the lowest employee turnover and countless benefits and privileges for working there. From free daycare to unlimited sick days‚ employees absolutely enjoy their
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