additional 15 minutes. The 65% cut was centrifuged for 15 minutes at 15‚000 xg. The supernatant was discarded. The pellet was resuspended in cold 4 mL of pH 7.5 phosphate buffer and transferred to a pre-chilled test tube for further purification. 200 uL was frozen down for later
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Cellular Biology Lab – Homework #3 Due the week of Nov. 4th You may use the lab manual‚ pre-lab lectures‚ and credible internet resources‚ however you may not use your cell bio lab classmates as a resource. You will most likely see this material again on the Final and I highly encourage you to work individually and seek help from myself or your TA. Plagiarism will result in an automatic zero. 1. In the cell bio lab‚ we use company manufactured gels‚ however you can make you own polyacrylamide
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The objective of the experiment was to differentiate mammalian C2C12 cells into muscle cells and analyze for presence of myosin light chain. Utilizing Western Blotting techniques and using specific antibodies helped to detect myosin light chain. The hypothesis was that proliferating myoblasts could be induced to initiate the differentiation process by depriving the cells of the necessary growth factors and that the differentiation is characterized by the expression of muscle-specific proteins. The
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of the most anticipating day in Islamic calendar is Eid-ul-Fitr and Eid-ul-Adha. While the former marks the end of fasting month of Ramadhan Al-Mubarak‚ Eid-ul-Adha marks the end of Hajj‚ the sacred pilgrimage to the holy city Mecca. It is compulsory for every able Muslim (as prescribed in the Five Pillars of Islam) to go on a Hajj at least once during his lifetime. Also popularly known as the Festival of Sacrifice‚ this Muslim holiday Eid-ul-Adha commemorates Prophet Abraham’s unselfish act of sacrificing
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About Eid Coming with the new moon‚ the festival marks the end of ’Ramadan’ - a month when Muslims fast throughout the day and eat only at night Prayers‚ feasts and family get- together are the major highlights of the eid ul fitr celebrations. It was during this month that the holy Koran was revealed. Eid means recurring happiness or festivity. Eid is celebrated in India with much enthusiasm and fervor and Muslims from all strata of life can be seen adorned in beautiful new clothes‚ visiting
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OUTLOOK The future plans of Dar-ul-Sukun reaches beyond the borders of religion‚ cast‚ color and creed. The had always perform to make its members self-sufficient‚ so that they can play a productive part in the society. The non-discriminatory attitude towards the society is a big example of promotion of humanity and brotherhood. They have always provided extension platform to build a community around shared values‚ interest and passion to bring change to the society. Dar-ul-Sukun has tirelessly strived
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Experiment 22 Laboratory to Biology III Diversity of Microorganisms / Wintersemester / page 1 Experiment 22 Isolation of plasmid-DNA from bacteria and PCR Advisor Konrad Egli: kegli@botinst.unizh.ch Reading Chapters in BBOM 10th: 10.8 BBOM : Madigan M.T.‚ J.M. Martinko and J. Parker: "Brock - Biology of Microorganisms"‚ 10th Edition (2003)‚ Prentice Hall. Objectives Background • Isolation of plasmid-DNA from different bacteria clones • Handling of bacteria clones
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Eid ul-Fitr means the celebration of the end of Ramadan. During Eid ul-Fitr we give donations to those in need in the community -- the donations are called Fitrana. The significance of this holiday is to celebrate the completion of Ramadan. Ramadan is the holy month of fasting‚ in which one eats before sun up and does not eat or drink again until sundown. This is one of the Five Pillars of Islam‚ which make up the foundation of Muslim life. The pillars consist of the oneness of God‚ five daily
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