LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
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PEES 4200W- Physiology of Exercise Lab #2: Metabolism and Energy Expenditure 1. The Respiratory exchange ratio is the ratio of the volume of carbon dioxide produced and oxygen consumed. The ratio indicates the energy that the subject is expending for indirect calorimetry‚ how efficient the subject ’s body is at utilizing the oxygen inhaled‚ as well as the main substrate being used for energy during varying intensities of exercise. During rest the volume of carbon dioxide was 0.73L/min‚ the volume
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Organic Chemistry II Lab 9 Fermentation of a Carbohydrate: Ethanol from Sucrose * Introduction Ethanol is one of the oldest alcohols and also the least toxic one. Industrially‚ ethanol is made most economically by hydration of ethylene. However‚ ethanol that is intended for human consumption must‚ by law‚ be prepared by fermentation. By either method‚ ethanol‚ of course‚ has the same formula‚ structure‚ and properties. The fermentation takes place with the assistance of enzymes from yeast
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enzyme concentration is decreased then the rate of reaction will decrease. Controlled: temperature Independent: % catalyze concentration ( Dependent: rate of reaction (mm/s) Materials: Mortar and pestle Graduated cylinder Distilled water Test tubes Chicken liver (or any other liver) Sterile transfer pipet (Dropper) Sterile weigh boat Hydrogen peroxide Razor blade Cutting board Electronic balance Timer Ruler Clean everything Calculate mass of chicken liver needed for 1ml‚ 5ml
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Lauren Sullivan Plants Imperfections Abstract The purpose of this experiment was to plant different crosses and observe the different phenotypic ratios the plants present. The procedure of this experiment was plant six different crosses and water them correctly so that we could observe the different phenotypes and compare them to Mendel’s proposed ratios. Mendel‚ who had studied peas‚ did a similar experiment and came up with specific ratios that a monohybrid and dihybrid cross should show. His
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Complexometric Determination of Water Hardness Kayla Partridge Britni Gonzales 09/15/2014 Abstract Determining the water hardness of unknown sample #55 was determined by complexometric titration. With the use of disodium salt EDTA as the solution to chelate the metal impurities and the Eriochrome Black T indicator as the solution used to help visualize when the impurities were completely chelated‚ along with a few other solutions to help the reaction. Unknown water sample #55 experimental
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1. OBJECTIVES (a) To determine the equivalent circuit parameters of a 3-phase squirrel-cage induction motor from laboratory test data. (b) To determine the performance characteristics of an induction motor under load conditions. (c) To control the no-load speed of an induction motor. 2. INTRODUCTION 2.1 Three-phase squirrel-cage induction motors An induction motor is a transformer with a rotating secondary winding. It has two essential components: an outer stationary stator and an
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EXPERIMENT NO. 1 INTRODUCTION TO LAB INSTRUMENTS. 1. BREADBOARD We should be familiar to the following things about a breadboard: * What is a breadboard and what is it used for? * How does it work? * Setting Up. * Limitations. What is a breadboard and what is it used for? A breadboard (or protoboard) is usually a construction base for prototyping of electronics. The term "breadboard" is commonly used to refer to a solderless breadboard (plugboard). It was designed by
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City University of Hong Kong Dept. of Physics & Materials Science AP2104 Mechanics of Solids Laboratory Manual Experiment 1 Pure Bending of a Beam Experiment 2 Torsional Deformations Experiment 3 Yield Criteria for Ductile Materials under Plane Stresses Experiment 1 Pure Bending of a Beam Objective 1. To examine the stresses at various positions of the beam under a constant load of pure bending. 2. To determine the curvature of deflection of the beam. Introduction
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substances precipitated through qualitative analysis. Solubility rules were also a major theme as solubility is important in determining whether a reaction will produce a precipitate. Starting out the experiment‚ HCl was added to the solution in the test-tube in order to form a reaction between the HCl‚ Ag‚ Pb‚ and Hg cations. The products of this addition of HCl were PbCl2‚ AgCl‚ and Hg2Cl2. After the HCl was added‚ the solution turned a milky white color. It was important to not add too much HCl
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