1. Describe the mechanism by which green fluorescent protein (GFP) is able to emit fluorescent light and why ultraviolet light is required to visualise it. (5 marks) When GFP is hit with UV light‚ the chromophore is hit by a photon. This changes the chromophore from ground state (A) to A*‚ which is a highly excitable state. Due to such a highly excitable state not being able to remain so for very long‚ the A* state chromophore emits a proton‚ lowering its state to I*‚ the energetic I. This I* state
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Hugh Kim Lab Report: Stoichiometry Lab 1. Prelab Part1. 1) Create no waste = The principle that encourages chemists to not create waste at the first place rather than cleaning it up afterwards effectively shifts the chemistry more environmentally conscious‚ as creating no waste would make the experiment efficient; the reactants will be reduced to only the essential ones and the product will be maximized‚ a change that would make the experiment economic. Also‚ if chemists aim to
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I. Introduction: The purpose of this lab report is to differentiate between of Newton’s Third Law and Newton’s Second Law. Newton’s Third Law states that all forces come in pairs and that the two forces in a pair act on different objects and are equal in strength and opposite in direction. Newton’s Second Law states that the acceleration of an object is proportional to the net force and inversely proportional to the mass of the object being accelerated. Using calculation equations for acceleration
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The Kinetics of α-Chymotrypsin Introduction Chymotrypsin is a protease which cleaves proteins by a hydrolysis reaction‚ it does this by adding a molecule of water to a peptide bond. Although the hydrolysis reaction is thermodynamically favoured in the absence of a catalyst the half-life for a typical hydrolysis reaction by a protease is between 10 and 100 years‚ needless to say it is extremely slow1. Though this is true peptide bonds are hydrolysed within milliseconds in the body in the presence
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Botany Lab ------------------------------------------------- Introduction This study observed the effects of different body fluids and solutions relative to breaking down bacteria‚ specifically in the human body. The enzymes we studied‚ lysozomes‚ help the body lyse‚ or break down bacteria by targeting peptidoglycan in bacterial walls. The solutions and fluids studied were saliva‚ mucus‚ tears‚ a stock solution of lysozomes‚ and distilled water. The solutions were placed in agar containing Micrococcus
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Lab Report Quiz!!! | | | LabRepQuiz010 Question MC #19: All are true of the Materials and Methods section except___.Answer | | | | | | | | | | * Question 2 1 out of 1 points | | | LabRepQuiz010 Question MC #7: Which of the following is the best written sentence that includes the information below taken from an article written by Dr. Costanza‚ but avoids plagiarism? -A decade of research from Sunshine Health Clinic has shown that 20 minutes of exercise a day can
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Laszlo Vass‚ Ed.D. Version 42-0027-00-01 Lab Report Assistant This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions‚ diagrams if needed‚ and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information in an editable
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D-82‚ Malviya Nagar‚ New Delhi-110 017 (Code No.1640) NIPSTEC COMPETENCY MAPPING IN HCL COMNET LTD (A Subsidiary of HCL Technologies) PROJECT By Project report submitted in partial fulfillment of the requirements for ‘Master of Business Administration’ Distance Education Wing‚ Syndicate House‚ Manipal-576 104 1 I hereby declare that the project report entitled: COMPETENCY MAPPING IN HCL COMNET LTD Submitted in partial fulfillment of the requirements for the degree of Master
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encoded by EZH2‚ the EZH2 gene encodes part of the Polycomb group which make protein complexes that help to maintain genes transcriptional repressive state over successive cell generations. http://www.ncbi.nlm.nih.gov/gene/2146 Throughout this lab report template DNA that contains the gene EZH2 was provided‚ this will be amplified by a PCR and cloned into a vector. This Polycomb group proteins help maintain the cell identity during progress through chromatin
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with a little ice-cold methanol‚ and suction dry‚ then air dry. Diaquobis(acetylacetonato)cobalt To a solution of 80 mg NaOH in 1 mL H2O‚ slowly add 200 mg (2 mmole) acetylacetone‚ with swirling. Any white solid formed at this point must be dissolved before continuing. This yellow solution is added dropwise‚ with stirring‚ to a solution of 238 mg (1 mmole) CoCl2.6H2O in 1.5 mL of water. Isolate the product by suction filtration and wash with water until the washings are nearly colorless
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