LSM1102 Lab Report Introduction Transformation is a process which involves plasmid DNA being bound to the cell surface and the subsequent uptake of DNA by the cell (Panja et al.‚ 2008). For artificial transformation of E. coli cells with plasmids‚ plasmid DNA has to be extracted from bacterial cells using the High-Speed Plasmid Mini Kit‚ which is then mixed with competent E. coli cells followed by heat shock and the streaking of transformed cells on two different types of agar plate (LB and LB+ampicillin)
Premium Plasmid Transformation Escherichia coli
The purpose of this lab is to determine the rate of reaction under different circumstances. Different variables will be manipulated to discover their effects on a particular reaction. Changes in temperature‚ pH‚ and enzyme concentration are examples of factors that have the potential to affect the initial rate of an enzyme catalyzed reaction in a controlled experiment‚ whether it be speeding the reaction up or slowing it down. Part I of the experiment establishes a baseline that can be used to compare
Premium Chemical reaction Chemistry Enzyme
Assuming Beer’s law holds A = ac (3.1) what are the values for %T (percent transmission) and a (absorption coefficient‚ in units of L/mol.cm) for the molecule at the above two wavelengths? Also do the following from Atkins. Exercise 9.16b Estimate the lifetime of a state that gives rise to a line of width a) 200. MHz‚ b) 2.45 cm-1. (Note that lifetime broadening is discussed in Section 9.6 of Atkins‚ pp 350-352. The key equation is E = /‚ where E is
Premium Electron Electron configuration Chemical bond
cheese. Lactic acid bacteria(LAB)‚ a bacteria that can be found in the production of cheese‚ its stress gene was investigated in the experiment by using various biochemical and genetic techniques to identify and extract. The characterisation of the strain illustrates how identification of strains differ using different methods‚ such as gram stain and 16s rRNA screening. After the characterisation‚ the stress gene isolation assist the further understanding of the gene on LAB be giving different stress
Premium Bacteria
EXPERIMENT NO. 1 INTRODUCTION TO LAB INSTRUMENTS. 1. BREADBOARD We should be familiar to the following things about a breadboard: * What is a breadboard and what is it used for? * How does it work? * Setting Up. * Limitations. What is a breadboard and what is it used for? A breadboard (or protoboard) is usually a construction base for prototyping of electronics. The term "breadboard" is commonly used to refer to a solderless breadboard (plugboard). It was designed by
Premium Voltage Direct current Alternating current
Lauren Sullivan Plants Imperfections Abstract The purpose of this experiment was to plant different crosses and observe the different phenotypic ratios the plants present. The procedure of this experiment was plant six different crosses and water them correctly so that we could observe the different phenotypes and compare them to Mendel’s proposed ratios. Mendel‚ who had studied peas‚ did a similar experiment and came up with specific ratios that a monohybrid and dihybrid cross should show. His
Premium Genetics Gregor Mendel Seed
Caffeine effect on Daphnia’s heart rate Prepared for: Androniki Kloni By: Maria Mitsi October 6‚ 2013 Abstract Caffeine is a bitter‚ white crystalline xanthine alkaloid and a stimulant drug. Caffeine is found in varying quantities in the seeds‚ leaves‚ and fruit of some plants‚ where it acts as a natural pesticide that paralyzes and kills certain insects feeding on the plants‚ as well as enhancing the reward memory of pollinators. An experiment was held‚ on an aquatic
Premium Caffeine Heart rate
music affect the heart rate and what different types of music have a greater affect on heart rate? Music most definitely has emotional value. Heart rate can be directly influenced by these emotional changes. Our group decided to test music tempo’s effect on heart rate. This experiment is relevant to one’s health because it is important to understand how to control one’s heart rate. Before we started conducting our experiment‚ we hypothesized that fast tempo music increases heart rates while slower
Premium Tempo Heart rate Hypothesis
whether alcohol and caffeine has an effect on heart rate‚ using the freshwater crustacean‚ Daphnia magna‚ as the experimental subject. For humans‚ alcohol works as a depressant‚ slowing down the heart rate of the consumer; caffeine works as a stimulant‚ increasing the heart rate of the consumer. The heart depends on an internal pacemaker system to keep it pumping consistently and at the right speed. Alcohol disturbs this pacemaker system and causes the heart to beat irregularly. Studies show that caffeine
Premium Blood Hypertension Heart
chemicals before use. Abide by specific warnings and directions. 3. Collect all materials needed for a procedure before proceeding. 4. Perform reactions under the hood when directed. Chemicals may be weighed and prepared at balance or lab tables‚ but tests should be carried out under the hood. 5.Acids and caustic chemicals are stored in the hood. Please do not take these chemicals from the hood. Procedure: PART 1: Metathetical reactions Precipitation reactions A1. Add a
Premium Chlorine Chemical reaction Sodium